Genetic Transformation of Pea (Pisum sativum)

Malysheva, N. V.; Pavlova, Z. B.; Chernysh, Natalia S.; Кравченко, Л В; Kislin, Y. N.; Chmelev, V. M.; Лутова, Л. А.

doi:10.1007/978-3-642-56901-2_19

Cited by 3 publications

(5 citation statements)

References 47 publications

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“…Pea transformation has already been reviewed in a number of journal papers or book chapters (Davies et al 1993;de Kathen & Jacobsen 1993;Atkins & Smith 1997;Malysheva et al 2001;Morton et al 2002;Grant & Cooper 2003;Somers et al 2003;Dita et al 2006;Bhowmik & Basu 2008;Nadolska-Orczyk 2008;Atif et al 2013;Gatti et al 2016), nevertheless, this review tries to provide a thorough summary of this topic.…”

Section: Conventional Pea Breeding Methods and Their Limitsmentioning

confidence: 99%

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Pea transformation: History, current status and challenges

Ludvíková¹,

Griga²

2022

Czech J. Genet. Plant Breed.

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This review recapitulates the history, important milestones, the current status, and the perspectives of the pea (Pisum sativum L.) transformation as a tool for pea crop breeding. It summarises the developments of the pea transformation from the first methodological experiments to achieving the complete transformation and regeneration of genetically modified (GM) plants, transformation with the first genes of interest (GOI), to recent techniques of targeted genome editing. We show how recent biotechnological methods and genetic engineering may contribute to pea breeding in order to speed up the breeding process and for the creation of new pea cultivars. The focus is laid on genetic engineering which represents an excellent technology to enhance the pea gene pool with genes of interest which are not naturally present in the pea genome. Different methods of pea transformation are mentioned, as well as various GOI that have been used for pea transformation to date, all aimed at improving transgenic pea traits. Tolerance to herbicides or resistance to viruses, fungal pathogens, and insect pests belong, among others, to the pea traits that have already been modulated by methods of genetic engineering. The production of phytopharmaceuticals is also an important chapter in the use of genetically modified peas. We compare different methods of introducing transgenes to peas and also the usage of different selective and reporter genes. The transformation of other major legumes (soybeans, beans) is marginally mentioned. The effect of genetically modified (GM) peas on animal health (feeding tests, allergenicity) is summarised, the potential risks and benefits of pea modification are evaluated and also the prime expectations of GM peas and the real current state of this technology are compared. Unfortunately, this technology or, more precisely, the products created by this technology are under strict (albeit not scientifically-based) legislative control in the European Union.

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Section: Conventional Pea Breeding Methods and Their Limitsmentioning

confidence: 99%

“…The pea has also been traditionally used as a classical model plant in fundamental studies related to genetics and plant breeding, biochemistry and molecular biology (Fehr 1993;Gaikwad et al 1999;Wen et al 1999;Malysheva et al 2001). The founding father of genetics, J.G.…”

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confidence: 99%

Pea transformation: History, current status and challenges

Ludvíková¹,

Griga²

2022

Czech J. Genet. Plant Breed.

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“…S nárůstem populace se navíc stále více přesouvá pozornost od živočišných bílkovin právě k rostlinným proteinům (Kristjansson et al 2014). Významnou roli sehráli zástupci luskovin také při studiu genetiky, šlechtění, biochemie či molekulární biologie (Fehr 1993, Gaikwad et al 1999, Wen et al 1999, Malysheva et al 2001. Za zmínku zcela jistě stojí J. G. Mendel jakožto zakladatel genetiky, který si pro svůj slavný výzkum záměrně vybral rod Pisum, protože zjednodušeně splňoval jeho tři základní požadavky, a to stálost znaků v průběhu generací (šlechtění), ochranu před cizím pylem během kvetení a nenarušenou plodnost hybridů a potomstva (Schwarzbach et al 2014).…”

Section: Cíl Metodikyunclassified

Zvýšení výnosu a nutriční hodnoty vybraných genotypů hrachu hnojením a agronomickou biofortifikací

al.¹

2022

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“…Recently, this research resulted in successful proof of pea insect and virus resistance in the field conditions (Morton et al 2000, Timmerman-Vaughan et al 2001. During last decade, the problem of pea transformation was discussed in several review papers (De Kathen and Jacobsen 1993, Malysheva et al 2001, Morton et al 2002, Grant and Cooper 2003.…”

Section: Introductionmentioning

confidence: 99%

“…A specific approach is in vivo transformation (DNA microinjection into intact nodal meristems followed by electroporation) − a technique which does not need an in vitro culture (Chowrira et al 1995). In in vitro systems, the protocols are mostly based on organogenesis in callus or on stimulation of proliferation of organized meristematic tissue contained in transformed explants (stem nodal explants, cotyledonary nodes, immature embryo segments/slices, immature cotyledons -see review of Malysheva et al 2001), only exceptionally on regeneration via somatic embryogenesis (Nicolas et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Pisum sativum in vitro and in vivo

Švábová¹,

Smýkal²,

Griga³

et al. 2005

Biologia plant.

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Six pea (Pisum sativum L.) cultivars (Adept, Komet, Lantra, Olivín, Oskar, Tyrkys) were transformed via Agrobacterium tumefaciens strain EHA105 with pBIN19 plasmid carrying reporter uidA (β-glucuronidase, GUS, containing potato ST-LS1 intron) gene under the CaMV 35S promoter, and selectable marker gene nptII (neomycin phosphotransferase II) under the nos promoter. Two regeneration systems were used: continual shoot proliferation from axillary buds of cotyledonary node in vitro, and in vivo plant regeneration from imbibed germinating seed with removed testa and one cotyledon. The penetration of Agrobacterium into explants during co-cultivation was supported by sonication or vacuum infiltration treatment. The selection of putative transformants in both regeneration systems carried out on media with 100 mg dm -3 kanamycin. The presence of introduced genes was verified histochemically (GUS assay) and by means of PCR and Southern blot analysis in T 0 putative transformants and their seed progenies (T 1 to T 3 generations). Both methods, but largely in vivo approach showed to be genotype independent, resulting in efficient and reliable transformation system for pea. The in vivo approach has in addition also benefit of time and money saving, since transgenic plants are obtained in much shorter time. All tested T 0 -T 3 plants were morphologically normal and fertile.

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Genetic Transformation of Pea (Pisum sativum)

Cited by 3 publications

References 47 publications

Pea transformation: History, current status and challenges

Pea transformation: History, current status and challenges

Zvýšení výnosu a nutriční hodnoty vybraných genotypů hrachu hnojením a agronomickou biofortifikací

Agrobacterium-mediated transformation of Pisum sativum in vitro and in vivo

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