Genetic Transformation of Roses, 2 Examples: One on Morphogenesis, the Other on Anthocyanin Biosynthetic Pathway

Somatic embryogenesis and transformation of the diploid Rosa chinensis cv Old Blush

“…Production of RcOBType1 embryogenic calli Most published protocols for rose transformation have used somatic embryos or somatic embryogenic callus as targets (Condliffe et al 2003;Derks et al 1995;Firoozabady et al 1994;Katsumoto et al 2007;Kim et al 2004;Li et al 2002Li et al , 2003Marchant et al 1998a, b;Souq et al 1996;Van der Salm et al 1997). Yet these protocols are genotypedependent and cannot be used readily with new varieties (Korban et al 2006).…”

“…Genetic transformation protocols for gene transfer and genetically modified rose production using reporter genes (such as b-glucuronidase, GUS) or genes of interest (disease resistance, color, etc.) have been reported (Condliffe et al 2003;Derks et al 1995;Dohm et al 2001aDohm et al , 2002Firoozabady et al 1994;Katsumoto et al 2007;Kim et al 2004;Li et al 2002Li et al , 2003Marchant et al 1998a, b;Souq et al 1996; Van der Salm et al 1997). The reports vary in the cultivars that were used, in the tissue source of the embryogenic callus, the reporter gene or the gene delivery system.…”

Transgenics in Ornamental Crops:Creating Novelties in Economically Important Cut Flowers

“…Chen et al 23 developed transgenic lines with enhanced freezing tolerance in China rose with the successful introduction of MtDREB1C gene, isolated form Medicago trancatula, without any abnormalities in existing plant characters. For alteration of plant morphology, studies 24,25 advocated the role of Rosa hybrida rolA, rolB and rolC genes, where insertion of rolC gene led to the production of dwarf plants in roses with small-sized, less fertile flowers of varying colours and numerous thorns, whereas insertion of rolA + B + C genes enhanced the rooting in cuttings with accompanying effects like reduced shoot length and apical dominance. Ito et al 26 demonstrated that Apple latent spherical virus (ALSV) vector infects roses without adversely affecting plant health and could be important for endogenous gene silencing in rose.…”

Genetic Transformation Protocol for Early Flowering Cryptochrome Gene in Taif Rose Plant

“…Regeneration from somatic embryos was first reported by (Souq et al, 1996) from calli of leaf, filaments and stamens. Subsequently, it was also reported from calli of leaf; filaments and immature seeds (Kunitake et al, 1993).…”

“…There are only a few published reports on Agrobacterium-mediated (Firoozabady et al, 1994;van der Salm et al, 1996) and bombardment mediated transformation in roses. Firooz abady et al, (1994) reported successful transformation of Rosa hybrida cv.…”