Genetic transformation of Stella De Oro daylily by particle bombardment

Abstract

S. 2003. Genetic transformation of Stella De Oro daylily by particle bombardment. Can. J. Plant Sci. 83: 873-876. Daylily (Hemerocallis sp. 'Stella de Oro') callus cultures initiated from ovules were bombarded with gold particles coated with plasmid harboring Basta ® resistance gene. Resulting putative transgenic calli were selected after 3 wk on semi-solid Murashige and Skoog's (MS) basal medium supplemented with 10 mg L -1 1-naphthaleneacetic acid, 2 mg L -1 6-benzylaminopurine and 3 mg L -1 phosphinothricin (PPT). Surviving calli regenerated shoots after 2 mo on semi-solid MS medium supplemented with 2 mg L -1 thiadiazuron and 1 mg L -1 PPT. Polymerase chain reaction and Southern blotting were used to confirm independent transformation events.

A new day dawning: Hemerocallis (daylily) as a future model organism

“…It has even been possible to make liquid suspension cultures of undifferentiated Hemerocallis cells and subsequently regenerate new plants from such material (Krikorian and Kann 1981), with the formation of embryogenic callus (Smith and Krikorian 1991). As might be expected, the amenability to tissue culture has assisted the development of systems for generating protoplasts and subsequent plant regeneration (Fitter and Krikorian 1981) and, most critically, transformation by particle bombardment (Aziz et al 2003). Transformation is exceptionally important as it permits reverse genetics, for example using specific gene silencing ‘knockdown’ technology such as hairpin RNAi and artificial microRNA.…”

Daylily: Botany, Propagation, Breeding

“…In vitro micropropagation can accelerate vegetative propagation. Daylilies can be micropropagated from young inflorescences (Meyer 1976;Pounders and Garton 1996), flower petals (Heuser and Apps 1976), ovaries (Krikorian and Kann 1980, 2002, suspension culture cells (Krikorian et al 1981b;Smith and Krikorian 1991), isolated protoplasts (Fitter and Krikorian 1981;Ling and Sauve 1995;Aziz et al 2003), anther filaments and immature seed embryos (Gulia and Carter 2007). Recently a liquid hioreactor system was developed for very large-scale propagation (Adelberg et al.…”

“…Regeneration protocols and genetic transformation of daylily (Hemer ocallis spp. 'Stella de Oro') by particle bombardment have been achieved (Aziz et al 2003). Callus cultures initiated from ovules were bombarded with gold particles coated with plasmid-harboring Bastaj resistance gene.…”