Genetic uniformity of international isolates of<i>Leifsonia xyli</i>subsp.<i>xyli</i>, causal agent of ratoon stunting disease of sugarcane

Young, Anthony; Petrasovits, L. A.; Croft, B. J.; Gillings, Michael R.; Brumbley, Stevens M.

doi:10.1071/ap06055

Cited by 28 publications

(24 citation statements)

References 33 publications

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“…3), but recently was reviewed and placed in the genus Leifsonia (Evtushenko et al 2000) based on rRNA gene analysis. This classification was recently confirmed by Young et al (2006).…”

Section: Introductionsupporting

confidence: 58%

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Recent advances in the molecular biology ofLeifsonia xylisubsp.xyli, causal organism of ratoon stunting disease

Brumbley

Petrasovits

Hermann

et al. 2006

Austral. Plant Pathol.

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Abstract. Twelve years ago our understanding of ratoon stunting disease (RSD) was confined almost exclusively to diagnosis of the disease and control via farm hygiene, with little understanding of the biology of the interaction between the causal agent (Leifsonia xyli subsp. xyli) and the host plant sugarcane (Saccharum spp. hybrids). Since then, research has focused on developing the molecular tools to dissect L. xyli subsp. xyli, so that better control strategies can be developed to prevent losses from RSD. Within this review, we give a brief overview of the progression in research on L. xyli subsp. xyli and highlight future challenges. After a brief historical background on RSD, we discuss the development of molecular tools such as transformation and transposon mutagenesis and discuss the apparent lack of genetic diversity within the L. xyli subsp. xyli world population. We go on to discuss the sequencing of the genome of L. xyli subsp. xyli, describe the key findings and suggest some future research based on known deficiencies that will capitalise on this tremendous knowledge base to which we now have access.

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“…3), but recently was reviewed and placed in the genus Leifsonia (Evtushenko et al 2000) based on rRNA gene analysis. This classification was recently confirmed by Young et al (2006).…”

Section: Introductionsupporting

confidence: 58%

“…xyli. Support for this hypothesis has been provided by the genetic diversity studies of Young et al (2006).…”

Section: Introductionmentioning

confidence: 70%

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Recent advances in the molecular biology ofLeifsonia xylisubsp.xyli, causal organism of ratoon stunting disease

Brumbley

Petrasovits

Hermann

et al. 2006

Austral. Plant Pathol.

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“…Bechet, 1995), 37% (Young et al, 2006) and 26% (Gagliardi and Camargo, 2009), respectively, 39 have been reported. The most evident symptom of RSD, as the name implies, is the impaired 40 development of the ratoon or stubble plants due to the reduction in the diameter of the stalks and 41…”

Section: 41% (Bailey and 38mentioning

confidence: 99%

Development of a qPCR for Leifsonia xyli subsp. xyli and quantification of the effects of heat treatment of sugarcane cuttings on Lxx

et al. 2016

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The main control practice of Leifsonia xyli subsp. xyli (Lxx) in sugarcane is to heat-treat cane 15 cuttings used as planting material in an attempt to eradicate the bacterium. A real time 16 quantitative PCR (qPCR) protocol specific for Lxx was developed to assess the effectiveness of 17 this practice. Primers were designed from the sequence of an Lxx-specific gene and detected as 18 were collected from plant canes with low bacterial titers whereas in the second they were 22 collected from first-ratoon canes with higher titers. Lxx was quantified in leaves by qPCR 90 23 *Manuscript Click here to view linked References 2 days after planting. Lxx was detected at variable titers in 50 to 90% of the plants, indicating that 24 the heat treatment was not effective in eradicating Lxx from all plants. However, in the second 25 experiment the bacterial population was reduced, as the median number of Lxx cells was lower 26 compared to the non-treated control. In the case of SP70-3370, the treatment also reduced the 27 number of Lxx-infected plants considering the pooled data of the two experiments. The results 28 indicated that although the heat treatment did not completely eliminate the bacterium, it can be 29 used to reduce the pathogen population in plants propagated from canes with high bacterial titers. 30

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“…While Actinobacteria are well represented by pure cultures, there is a great diversity of undescribed species within this phylum in Australian arid soils, warranting further investigation (18). Of particular interest was Leifsonia xyli (mCFU65), a slow-growing, small coryneform and gram-positive phytopathogen thought to be widespread in the environment but which had, until these experiments, never been directly isolated outside the host cell (6,7,31). The remaining isolates were most similar to bacteria within the Proteobacteria, including mCFU17, a putative novel species most similar to Novosphingobium subterraneum (Table 1).…”

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confidence: 99%

Cultivation of Fastidious Bacteria by Viability Staining and Micromanipulation in a Soil Substrate Membrane System

Ferrari

Gillings

2009

Appl Environ Microbiol

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Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.

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Genetic uniformity of international isolates ofLeifsonia xylisubsp.xyli, causal agent of ratoon stunting disease of sugarcane

Cited by 28 publications

References 33 publications

Recent advances in the molecular biology ofLeifsonia xylisubsp.xyli, causal organism of ratoon stunting disease

Recent advances in the molecular biology ofLeifsonia xylisubsp.xyli, causal organism of ratoon stunting disease

Development of a qPCR for Leifsonia xyli subsp. xyli and quantification of the effects of heat treatment of sugarcane cuttings on Lxx

Cultivation of Fastidious Bacteria by Viability Staining and Micromanipulation in a Soil Substrate Membrane System

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