Genetic Variability among Populations of the Florida Grasshopper Sparrow

Delany, Michael F.; Giesel, James T.; Brazeau, Daniel A.

doi:10.2307/3802733

Cited by 13 publications

(9 citation statements)

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“…1). There is substantial overlap between the Florida samples analysed here and those genotyped by Delany et al . (2000).…”

Section: Methodsmentioning

confidence: 84%

“…Sequences used to design primers for these three loci have been deposited in GenBank under Accession nos –. The six loci are different from those used by Delany et al . (2000).…”

Section: Methodsmentioning

confidence: 91%

“…As a result this subspecies received official US endangered status in 1986 (Federal Register 1986). Previous genetic work has examined levels of differentiation among subpopulations of floridanus (Delany et al. 2000), but to date no genetic analyses have been conducted which examine the genetic distinctiveness of floridanus relative to the other less‐threatened subspecies.…”

Section: Introductionmentioning

confidence: 99%

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Ancestral polymorphisms in genetic markers obscure detection of evolutionarily distinct populations in the endangered Florida grasshopper sparrow (Ammodramus savannarum floridanus)

et al. 2003

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Genetic analyses of bird subspecies designated as conservation units can address whether they represent units with independent evolutionary histories and provide insights into the evolutionary processes that determine the degree to which they are genetically distinct. Here we use mitochondrial DNA control region sequence and six microsatellite DNA loci to examine phylogeographical structure and genetic differentiation among five North American grasshopper sparrow (Ammodramus savannarum) populations representing three subspecies, including a population of the endangered Florida subspecies (A. s. floridanus). This federally listed taxon is of particular interest because it differs phenotypically from other subspecies in plumage and behaviour and has also undergone a drastic decline in population size over the past century. Despite this designation, we observed no phylogeographical structure among populations in either marker: mtDNA haplotypes and microsatellite genotypes from floridanus samples did not form clades that were phylogenetically distinct from variants found in other subspecies. However, there was low but significant differentiation between Florida and all other populations combined in both mtDNA (FST = 0.069) and in one measure of microsatellite differentiation (theta = 0.016), while the non-Florida populations were not different from each other. Based on analyses of mtDNA variation using a coalescent-based model, the effective sizes of these populations are large (approximately 80,000 females) and they have only recently diverged from each other (< 26,000 ybp). These populations are probably far from genetic equilibrium and therefore the lack of phylogenetic distinctiveness of the floridanus subspecies and minimal genetic differentiation is due most probably to retained ancestral polymorphism. Finally, levels of variation in Florida were similar to other populations supporting the idea that the drastic reduction in population size which has occurred within the last 100 years has not yet had an impact on levels of variation in floridanus. We argue that despite the lack of phylogenetic distinctiveness of floridanus genotypes the observed genetic differentiation and previously documented phenotypic differences justify continued designation of this subspecies as a protected population segment.

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“…1). There is substantial overlap between the Florida samples analysed here and those genotyped by Delany et al . (2000).…”

Section: Methodsmentioning

confidence: 84%

“…Sequences used to design primers for these three loci have been deposited in GenBank under Accession nos –. The six loci are different from those used by Delany et al . (2000).…”

Section: Methodsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Ancestral polymorphisms in genetic markers obscure detection of evolutionarily distinct populations in the endangered Florida grasshopper sparrow (Ammodramus savannarum floridanus)

et al. 2003

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“…The high allelic diversity found in the studied area is not in accordance with a small population size, and long distance dispersal of individuals between reproductive sites along feeding and transit areas may lead to the Wahlund effect. However, the use of small sample sizes when studying highly polymorphic loci may also prevent the detection of the true levels of heterozygosity (DELANY et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Microsatellite genotyping from faeces of Lontra longicaudis from southern Brazil

Weber¹,

Hildebrand

Ferreira

et al. 2009

Iheringia, Sér. Zool.

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ABSTRACT.A genetic study of the neotropical river otter Lontra longicaudis (Olfers, 1818), which has an unknown conservation status, was carried out at the Taim Ecological Station and the margins of the Vargas stream, Rio Grande do Sul, southern Brazil. Faecal samples were collected, and DNA was extracted using a silica-guanidine method. Five microsatellite loci were amplified using PCR with heterologous primers previously described for Lutra lutra (Linnaeus, 1758). Sixteen faecal samples out of 29 from Taim and 11 out of 14 from Vargas stream margins contained enough DNA for genetic analysis. A total of 49 different alleles were found at both localities, from which 18 were exclusively found in individuals from Taim and 17 were exclusives from Vargas individuals. The most common allele was the same at both locations for three loci (Lut715, Lut733, and Lut818). A high level of genetic diversity was found at both sites (Ne Taim =4.1, Ho Taim =0.299, He Taim =0.681; Ne Vargas =4.9, Ho Vargas =0.355, He Vargas =0.724), being higher at the Vargas stream site. A high and significant level of heterozygote deficiency was observed at most loci according to the χ 2 test. The homogeneity χ 2 test (P≤0.001) showed that there were significant differences in the allele frequencies between the two locations. Genotyping for more than one locus was possible in 81.5% of samples, from which only 37% were possible to genotype for more than three loci. A low degree of relatedness was found among individuals from Taim (R=0.055±0.310), but an even lower value of relatedness was found at the Vargas site (R= -0.285±0.440). The significant degree of differentiation (I=0.890; F ST =0.059) found between Taim and Vargas individuals suggests that there is more than one population of otters in the southern extreme of Brazil, which probably are associated with the water body systems found in this region, the Mirim and the Caiuvá/Flores/Mangueira Lagoons. The high genetic diversity and low relatedness found at the Vargas stream, lead us to believe that the Vargas stream may be acting as a corridor between these water bodies for otter dispersion. KEYWORDS.Neotropical river otter, genetic markers, Taim, non-invasive sampling, genetic diversity.RESUMO. Genotipagem através de microsatélites extraídos de amostras fecais em Lontra longicaudis do sul do Brasil. A lontra neotropical de rio Lontra longicaudis (Olfers, 1818), cujo estado de conservação é ainda desconhecido, foi estudada geneticamente na Estação Ecológica do Taim e nas margens do arroio Vargas, RS, sul do Brasil. Amostras de fezes foram coletadas e o DNA foi extraído por um método de sílica-guanidina. Cinco locos de microsatélites foram amplificados por PCR utilizando primers heterólogos previamente descritos para Lutra lutra (Linnaeus, 1758). Dezesseis amostras de fezes de um total de 29 coletadas no Taim e onze das 14 obtidas no arroio Vargas contiveram DNA suficiente para prosseguir com a analise genética. Um total de 49 alelos foram obtidos, dos quais 18 foram exclusivos de indivíduos...

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“…Over the last 20 years, numerous studies of FGSPs have been conducted. This includes studies of their distribution and abundance (Delany et al 1985, 2007b), demography (Delany et al 1993, Dean et al 1998, Pranty 2000, Perkins and Vickery 2001), population genetics (Delany et al 2000a, Bulgin et al 2003, Mylecraine et al 2008), territory size and movement patterns (Delany et al 1995), diet (Delany et al 2000b), nonbreeding season ecology (Dean 2001), habitat requirements (Delany et al 1985, Shriver 1996, Delany and Linda 1998, Tucker and Bowman 2006), and response to fire (Walsh et al 1995, Shriver et al 1996, 1999, Shriver and Vickery 2001, Delany et al 2002). In addition, management plans have been developed for each of the three public properties (hereafter referred to as management areas) where they occur (U.S.…”

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confidence: 99%

Metapopulation structure, population trends, and status of Florida Grasshopper Sparrows

Tucker

Schrott

Delany

et al. 2010

Journal of Field Ornithology

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P < 0.001). Estos patrones sugieren que existe una metapoblación de A. s. floridanus cual consiste de tres poblaciones distintas (APAFR, KPPSP, y TLWMA), y que las agregaciones espacialmente distintas en APAFR constituyen una población cual utiliza diferentes parches de hábitat. La población en APAFR esta bajo riesgo de extirpación, y se necesita de acción inmediata para recuperarla. Tomando en cuenta las dinámicas metapoblaciones mas amplias será util para guiar los esfuerzos de manejo enfocados en la conservación de A. s. floridanus en el paisaje del centro de la Florida.

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Genetic Variability among Populations of the Florida Grasshopper Sparrow

Cited by 13 publications

References 0 publications

Ancestral polymorphisms in genetic markers obscure detection of evolutionarily distinct populations in the endangered Florida grasshopper sparrow (Ammodramus savannarum floridanus)

Ancestral polymorphisms in genetic markers obscure detection of evolutionarily distinct populations in the endangered Florida grasshopper sparrow (Ammodramus savannarum floridanus)

Microsatellite genotyping from faeces of Lontra longicaudis from southern Brazil

Metapopulation structure, population trends, and status of Florida Grasshopper Sparrows

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