Grasslands are often grazed by cattle and many grassland birds nest on the ground, potentially exposing nests to trampling. We tested for trampling risk introduced by cattle to nests of endangered Florida Grasshopper Sparrows (Ammodramus savannarum floridanus) using experimentally paired grids of artificial nests (i.e., clay targets) similar in size to nests of Florida Grasshopper Sparrows and counted the number of clay targets that were broken in paired grazed and ungrazed enclosures. Clay targets in grazed grids were trampled 3.9% more often than their respective ungrazed grids, and measurements of cattle presence or density were correlated with the number of broken clay targets, suggesting that excluding cattle during breeding is an important management recommendation for the Florida Grasshopper Sparrow. Trampling rates within grazed enclosures were spatially homogeneous with respect to cattle infrastructure such as supplemental feeding troughs and fences, and forests and stocking density were poor predictors of trampling rates when excluding ungrazed grids. We used population viability analysis to compare quasi-extinction rates, intrinsic growth rates, and median abundance in grazed and ungrazed Florida Grasshopper Sparrow aggregations to further understand the biological significance of management aimed at reducing trampling rates during the breeding season. Simulations indicated that trampling from grazing increased quasi-extinction rates by 41% while reducing intrinsic growth rates by 0.048, and reducing median abundance by an average of 214 singing males after 50 years. Management should avoid grazing enclosures occupied by Florida Grasshopper Sparrows during the nesting season to minimize trampling rates. Our methods that combine trampling experiments with population viability analysis provide a framework for testing effects from trampling on other grassland ground-nesting birds, and can directly inform conservation and management of the Florida Grasshopper Sparrow. Évaluation du risque de piétinement par le bétail de nids de passereaux menacés à partir d'expériences avec des nids artificiels et de simulations RÉSUMÉ. Les prairies sont souvent broutées par le bétail et de nombreux oiseaux de prairie nichent au sol, exposant ainsi potentiellement leur nid au piétinement. Nous avons testé le risque de piétinement causé par le bétail des nids du Bruant sauterelle de Floride (Ammodramus savannarum floridanus), une espèce menacée, au moyen de grilles expérimentales appariées de nids artificiels (c.à-d. disques d'argile), semblables en taille aux nids des Bruants sauterelles de Floride. Nous avons compté le nombre de disques d'argile qui ont été détruits dans les enclos appariés broutés ou non broutés. Les disques d'argile situés dans les grilles broutées ont été piétinés 3,9 % plus souvent que ceux qui sont sis dans les grilles non broutées appariées, et les mesures de la présence du bétail ou de la densité étaient corrélées avec le nombre de disques détruits; ces résultats nous mènent à recommander fortement...