Genetic variability and diversity of the main resources of lily assessed via phenotypic characters, pollen morphology, and ISSR markers

Wang, J.M.; Ma, S.L.Y.; Li, W.Q.; Wang, Q.; He, Chun; Gu, Jianxun; Lu, Yingzhi

doi:10.4238/gmr.15027638

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…Lily belongs to the genus Lilium of the family Liliaceae. It is a summer‐blooming perennial bulbous plant with various food, aesthetic, medicinal and economic values (Wang et al , ). The fleshy bulb scales are rich in nutrients and antioxidants (Jin et al , ).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling uncovers a Lilium regale NAC transcription factor, LrNAC35, contributing to defence response against cucumber mosaic virus and tobacco mosaic virus

Sun

Zhang

et al. 2019

Molecular Plant Pathology

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Summary Cucumber mosaic virus (CMV) is a highly prevalent viral pathogen causing substantial damage to the bulb and cut‐flower production of Lilium spp. Here, we performed an Illumina RNA sequencing (RNA‐Seq) study on the leaf tissues of a virus‐resistant species Lilium regale inoculated with mock control and CMV. A total of 1346 differentially expressed genes (DEGs) were identified in the leaves of L. regale upon CMV inoculation, which contained 34 up‐regulated and 40 down‐regulated DEGs that encode putative transcription factors (TFs). One up‐regulated TF, LrNAC35, belonging to the NAM/ATAF/CUC (NAC) superfamily, was selected for further functional characterization. Aside from CMV, lily mottle virus and lily symptomless virus infections provoked a striking increase in LrNAC35 transcripts in both resistant and susceptible Lilium species. The treatments with low temperature and several stress‐related hormones activated LrNAC35 expression, contrary to its reduced expression under salt stress. Ectopic overexpression of LrNAC35 in petunia (Petunia hybrida) resulted in reduced susceptibility to CMV and Tobacco mosaic virus infections, and enhanced accumulation of lignin in the cell walls. Four lignin biosynthetic genes, including PhC4H, Ph4CL, PhHCT and PhCCR, were found to be up‐regulated in CMV‐infected petunia lines overexpressing LrNAC35. In vivo promoter‐binding tests showed that LrNAC35 specifically regulated the expression of Ph4CL. Taken together, our results suggest a positive role of transcriptome‐derived LrNAC35 in transcriptional modulation of host defence against viral attack.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling uncovers a Lilium regale NAC transcription factor, LrNAC35, contributing to defence response against cucumber mosaic virus and tobacco mosaic virus

Sun

Zhang

et al. 2019

Molecular Plant Pathology

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“…China is considered as a center of origin for Lilium , yet a small number of studies have been carried out on the researches of wild Lilium ’ evaluation and utilization, especially abiotic tolerances. Previous studies about Chinese Lilium have focused on morphological characterization ( Niu et al., 2010 ; Xiang et al., 2005 ), taxonomic ( Liu et al., 2017 ), phylogenetic ( Kim et al., 2019 ; Zhou et al., 2020 ), genetic variability, and diversity ( Wang et al., 2016 ). Thus, we want to develop a comprehensive understanding of the realized niche of Lilium in this study, which can provide a new insight into the tolerance evaluation of Lilium .…”

Section: Discussionmentioning

confidence: 99%

Prediction of temperature tolerance in Lilium based on distribution and climate data

Chai

Zhang

et al. 2021

iScience

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There are plenty publications providing guidance for resistant taxa selection by experimental researches while the number of experimental taxa is often restricted. In this study, we presented a concise method to predict the temperature tolerance of wild Lilium in China based on open access botanical and associated environmental datasets. We divided all taxa into five groups to present an overview of Lilium's adaptability to temperature stress. Furthermore, according to the environmental conditions, the prediction of heat and cold tolerance in Lilium was made based on the combined multi-sources data at taxon level. Thirteen taxa with potential temperature tolerance were predicted of 42 taxa. The results showed that not only is tolerance prediction created by large-scale data analysis possible, but that it may supplement traditional laboratory researches with a comprehensive list of taxa.

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“…Normal pollen development is essential for ensuring the high yield and quality of crops and fruits, but deleterious effects are often inevitable. For example, many ornamental plants (e.g., Populus, lily, and chrysanthemum) contain high concentrations of pollen, which results in pollen contamination and anaphylactic reactions in susceptible populations and reduces the commercial value of these ornamental plants (Tong et al, 2013;Sui et al, 2015;Wang et al, 2016;Kim et al, 2018;Feng et al, 2019;Li et al, 2019;Teng et al, 2021). Thus, male-sterile or pollen-free ornamental plants are often highly useful.…”

Section: Introductionmentioning

confidence: 99%

A Novel R2R3-MYB Gene LoMYB33 From Lily Is Specifically Expressed in Anthers and Plays a Role in Pollen Development

Feng

Yuan

et al. 2021

Front. Plant Sci.

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Lily (Lilium spp.) is an important commercial flower crop, but its market popularity and applications are adversely affected by severe pollen pollution. Many studies have examined pollen development in model plants, but few studies have been conducted on flower crops such as lily. GAMYBs are a class of R2R3-MYB transcription factors and play important roles in plant development and biotic resistance; their functions vary in different pathways, and many of them are involved in anther development. However, their function and regulatory role in lily remain unclear. Here, the GAMYB homolog LoMYB33 was isolated and identified from lily. The open reading frame of LoMYB33 was 1620 bp and encoded a protein with 539 amino acids localized in the nucleus and cytoplasm. Protein sequence alignment showed that LoMYB33 contained a conserved R2R3 domain and three BOX motifs (BOX1, BOX2, and BOX3), which were unique to the GAMYB family. LoMYB33 had transcriptional activation activity, and its transactivation domain was located within 90 amino acids of the C-terminal. LoMYB33 was highly expressed during the late stages of anther development, especially in pollen. Analysis of the promoter activity of LoMYB33 in transgenic Arabidopsis revealed that the LoMYB33 promoter was highly activated in the pollen of stage 12 to 13 flowers. Overexpression of LoMYB33 in Arabidopsis significantly retarded growth; the excess accumulation of LoMYB33 also negatively affected normal anther development, which generated fewer pollen grains and resulted in partial male sterility in transgenic plants. Silencing of LoMYB33 in lily also greatly decreased the amount of pollen. Overall, our results suggested that LoMYB33 might play an important role in the anther development and pollen formation of lily.

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Genetic variability and diversity of the main resources of lily assessed via phenotypic characters, pollen morphology, and ISSR markers

Cited by 11 publications

References 20 publications

Comparative transcriptome profiling uncovers a Lilium regale NAC transcription factor, LrNAC35, contributing to defence response against cucumber mosaic virus and tobacco mosaic virus

Comparative transcriptome profiling uncovers a Lilium regale NAC transcription factor, LrNAC35, contributing to defence response against cucumber mosaic virus and tobacco mosaic virus

Prediction of temperature tolerance in Lilium based on distribution and climate data

A Novel R2R3-MYB Gene LoMYB33 From Lily Is Specifically Expressed in Anthers and Plays a Role in Pollen Development

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