Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni

Vidigal, Teofânia H. D. A.; Neto, Elias David; Spatz, Linus; Nunes, Diana Noronha; Pires, Edina R.; Simpson, Andrew J.G.; Carvalho, Omar S.

doi:10.1590/s0074-02761998000700014

Cited by 11 publications

(7 citation statements)

References 43 publications

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“…Previously, we have explored other molecular methods to obtain species-specific markers. Initially we observed that the arbitrarily primed-PCR (AP-PCR) mirrored the high genetic variability in B. glabrata species (Vidigal et al 1994(Vidigal et al , 1998b. These results demonstrated that this methodology does not provide good markers for molecular identification of Biomphalaria species.…”

Section: Discussionmentioning

confidence: 99%

Further studies on the molecular systematics of Biomphalaria snails from Brazil

Vidigal

Caldeira

Simpson³

et al. 2000

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 99%

Further studies on the molecular systematics of Biomphalaria snails from Brazil

Vidigal

Caldeira

Simpson³

et al. 2000

Mem. Inst. Oswaldo Cruz

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“…Phylogenetic reconstruction of the Biomphalaria species, based on homologous sequence data, is well supported by (Mello 1972), substantiating the contention that African species are younger than their Neotropic congeners. Similarly, the remarkably high levels of intraspeci¢c genetic variability of South American Biomphalaria species, particularly B. glabrata (Vidigal et al 1994(Vidigal et al , 1998, has led to di¤culties in generating molecular markers for species identi¢cation. Woodru¡ & Mulvey (1997) concluded from allozyme data that B. glabrata is either the most genetically variable mollusc species yet described or a syngameon of more than three semispecies.…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence supports an African affinity of the Neotropical freshwater gastropod,Biomphalaria glabrata, Say 1818, an intermediate host forSchistosoma mansoni

Campbell

Jones

Lockyer

et al. 2000

Proc. R. Soc. Lond. B

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Freshwater snails of the genus Biomphalaria, Preston 1910, are the most important and widely distributed intermediate hosts of Schistosoma mansoni, the blood fluke responsible for human intestinal schistosomiasis, in Africa and the Neotropics. S. mansoni is thought to have been imported repeatedly into the Americas during the last 500 years with the African slave trade. Surprisingly considering that the New and Old World separated 95-106 million years (Myr) ago, the disease rapidly became established due to the presence of endemic susceptible hosts. Reconstructing the phylogenetic relationships within Biomphalaria may provide insights into the successful intercontinental spread of S. mansoni. Parsimony and distance analyses of mitochondrial and nuclear sequences show African taxa to be monophyletic and Neotropical species paraphyletic, with Biomphalaria glabrata forming a separate clade from other Neotropical Biomphalaria, and ancestral to the African taxa. A west to east trans-Atlantic dispersal of a B. glabrata-like taxon, possibly as recently as the Plio-Pleistocene (1.8-3.6 Myr ago) according to a general mitochondrial clock, would fit these observations. Vicariance or an African origin for B. glabrata followed by multiple introductions to South America over the past 500 years with the African slave trade seem unlikely explanations. Knowledge of the phylogenetic relationships among important intermediate host species may prove useful in furthering control measures which exploit genetic differences in susceptibility to parasites, and in elucidating the evolution of schistosome resistance.

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“… PCR - RFLP analysis of mitochondrial and nuclear genes ( Spatz et al 1999 ; Vidigal et al 1998 , 2000 ; Caldeira et al 2000 , 2009 ), fingerprinting techniques using nonspecific primers ( Abdel-Hamid et al 1999 ; Al-Quraishy et al 2014 ) and sequence analysis of COI and r16RNA genes and ITS-1 and ITS-2 sequences ( Woolhouse and Chandiwana 1989 ; Langand et al 1998 ; Vidigal et al 2000 ; Campbell et al 2000 ; DeJong et al 2003 ; Wethington et al 2007 ; Tuan and Santos 2007 ; Tuan et al 2012 ) have all produced results that allowed significant genetic differences in species and populations to be identified.…”

Section: Introductionmentioning

confidence: 99%

Molecular and morphological identification of Biomphalaria species from the state of São Paulo, Brazil

Palasio¹,

Guimarães²,

Ohlweiler³

et al. 2017

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DNA barcoding and morphological characters were used to identify adult snails belonging to the genus Biomphalaria from 17 municipalities in the state of São Paulo, Brazil. The DNA barcode analysis also included twenty-nine sequences retrieved from GenBank. The final data set of 104 sequences of the mitochondrial cytochrome oxidase I (COI) gene was analyzed for K2P intraspecific and interspecific divergences, through tree-reconstruction methods (Neighbor-Joining, Maximum Likelihood and Bayesian inference), and by applying different models (ABGD, bPTP, GMYC) to partition the sequences according to the pattern of genetic variation. Twenty-seven morphological parameters of internal organs were used to identify specimens. The molecular taxonomy of Biomphalaria agreed with the morphological identification of specimens from the same collection locality. DNA barcoding may therefore be a useful supporting tool for identifying Biomphalaria snails in areas at risk for schistosomiasis.

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Genetic variability and identification of the intermediate snail hosts of Schistosoma mansoni

Abstract: Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in

Cited by 11 publications

References 43 publications

Further studies on the molecular systematics of Biomphalaria snails from Brazil

Further studies on the molecular systematics of Biomphalaria snails from Brazil

Molecular evidence supports an African affinity of the Neotropical freshwater gastropod,Biomphalaria glabrata, Say 1818, an intermediate host forSchistosoma mansoni

Molecular and morphological identification of Biomphalaria species from the state of São Paulo, Brazil

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