Genetic variability for total phenolics, flavonoids and antioxidant activity of testaless seeds of a peanut recombinant inbred line population and identification of their controlling QTLs

Abstract

Peanut, an important oil producing crop contains bioactive compounds like phenolics and flavonoids which determine its antioxidative activity. We report here the genetic variability for total phenolics, flavonoids and DPPH radical scavenging activity of testaless seeds of a peanut recombinant inbred line population. On an average, the population contained 1.65 mg GAE/g and 240 lg CE/g of phenolics and flavonoids, respectively in its seed. The DPPH radical scavenging activity of this population ranged from 1.27 to 4.40 mM of TEAC/g of seed with an average of 2.87 mM of TEAC/g and had significant positive correlation with both phenolics and flavonoids content. Genetic mapping and QTL analysis revealed five QTLs for total flavonoid content, four QTLs for DPPH radical scavenging activity and a single QTL for total phenolic content in six linkage groups. Of these 10 QTLs, six were positioned in linkage group A02 and A03 wherein rust and late leaf spot resistance genes were located.

Evaluation of total flavonoid content and analysis of related EST-SSR in Chinese peanut germplasm

“…Hassan et al (2016) transformated chitinase gene into peanut to enhance resistance to leaf spot. A RIL population was also used for the initial location of QTLs for peanut TFC by Mondal et al (2015), but further research on this topic is required.…”

“…On the basis of 50 extant QTLs for isoflavone in soybean, Wang et al (2015) located 21 new QTLs using a RIL population, including many sites related to the environment. In peanut, Mondal et al (2015) located five QTLs for flavonoid content using a RIL population, including pPGPseq_12A07-UBC 841, GM 633-Ah242, RM14E11-TE 389, ARS 796-SSR_HO115593, and S89-TC11D09, with a minimum distance between markers of 21.0 cM. In this study, 215 pairs of EST-SSR markers were used to determine genetic differences in the 57 accessions.…”

Co-localization of major quantitative trait loci for pod size and weight to a 3.7 cM interval on chromosome A05 in cultivated peanut (Arachis hypogaea L.)

“…In recent years, QTLs associated with economically important traits such as disease resistance [8, 9], drought tolerance [10, 11], seed and oil quality [12, 13], agronomic and yield traits [14, 15] were identified in peanut crop. Molecular markers tightly linked to QTLs after validation can be further deployed in GAB [5, 16].…”

Classical and Molecular Approaches for Mapping of Genes and Quantitative Trait Loci in Peanut

“…Another study reported 30 major QTLs for saturated fatty acids (Wang et al 2015). In addition to this, ten major QTLs were reported with the PVE% range 12.5, 25-67 and 11.5-33 for the total phenolics, total flavonoids and DPPH radical scavenging, respectively by the Mondal et al (2015). The linked markers are available for use in breeding to improve the fatty acid profiles in peanut.…”

“…In peanut, ICRISAT with the research partners has done pioneer work to identify linked markers for drought tolerance related traits. In this context, major QTLs were identified successfully for transpiration (5 QTLs), transpiration efficiency (4 QTLs), carbon discrimination ratio (1 QTL), specific leaf area (6 QTLs), leaf area (1 QTL), SPAD chlorophyll Mondal et al (2015) metre reading (SCMR) (12 QTLs), biomass (3 QTLs), shoot dry weight (2 QTLs), haulm weight (1 QTL), harvest index (1 QTL), canopy conductance (3 QTLs) and total dry matter (1 QTL) (Varshney et al 2009b;Ravi et al 2011;Gautami et al 2012a). The another study while dissecting drought tolerance traits Fonceka et al (2012) identified two major QTLs each for 100 pod weight, 100 seed weight, haulm weight, pod number, total biomass, STI-100 pod weight, STI-100 seed weight, STI-haulm weight, STI-pod number, STI-total biomass, one major QTL each for STI-pod weight, STI-seed number and STI-seed weight.…”