Genetic variability of apple proliferation phytoplasmas as determined by PCR-RFLP and sequencing of a non-ribosomal fragment

Jarausch, W.; Saillard, Colette; Helliot, B; Garnier, M.; Dosba, F.

doi:10.1006/mcpr.1999.0279

Cited by 52 publications

(61 citation statements)

References 12 publications

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“…P. mali' over large parts of its distribution range and to reveal the pathological importance of distinct strains (cainelli et al 2004;casati et al 2010;Danet et al 2011;Jarausch et al 2000;Martini et al 2008;Schneider and Seemüller 2009;Seemüller and Schneider 2007;Seemüller et al 2010). in order to analyse the epidemiology and spread of aP in South tyrol, a method for simultaneous typing of 'Ca.…”

Section: Molecular Typing Of 'Ca P Mali'mentioning

confidence: 99%

“…P. mali' at two genetic loci on an automated sequencing system was established (Baric et al 2011a). the two loci comprised a putative rhodaneselike protein gene that displays two point mutations which allow differentiation of three different subtypes (Jarausch et al 2000) and the ribosomal protein l22 gene which carries a variable copy number of a 12-nucleotide repeat sequence, and based on the number of copies, four different subtypes could previously be distinguished (Martini et al 2008).…”

Section: Molecular Typing Of 'Ca P Mali'mentioning

confidence: 99%

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Molecular Tools Applied to the Advancement of Fruit Growing in South Tyrol: a Review

Baric

2012

Erwerbs-Obstbau

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Molecular biology techniques have become indispensable tools in various areas of research and routine applications. in South tyrol (northern italy), molecular methods have been applied to the field of fruit growing over the last decade, and the main applications have been related to plant pathology and identification of apple cultivars. this review article illustrates how the implementation of existing methods and the development of new assays have contributed to gain more insights about the spread and epidemiology of phytoplasma diseases affecting apple trees and grapevine in South tyrol, to identify and characterise the causal agents of newly emerging disorders, or to describe the local genetic diversity of the apple. Anwendung molekularbiologischer Methoden im Obstbau Südtirols - ein ÜberblickZusammenfassung Molekularbiologische Methoden haben sich zu einem unverzichtbaren Hilfsmittel in verschiedenen Bereichen der Forschung und in routineanwendungen entwickelt. in Südtirol (norditalien) werden molekularbiologische Methoden seit einem Jahrzehnt im Bereich des Obstbaus angewandt, mit Schwerpunkt auf Pflanzenkrankheiten und die identifizierung von apfelsorten. Dieser review zeigt den Beitrag der implementierung bestehender Verfahren und der entwicklung neuer Methoden zur gewinnung profunder erkenntnisse über die ausbreitung und epidemiologie von Phytoplasmen-Krankheiten des apfels und der rebe in Südtirol, zur identifizierung und charakterisierung neuer Schaderreger sowie zur Beschreibung der lokalen genetischen Vielfalt des apfels.Schlüsselwörter apfeltriebsucht · Schwarzholzkrankheit · Weißdornblattsauger · Sommerapfelblattsauger · 'Candidatus Phytoplasma mali' · 'Candidatus Phytoplasma prunorum' · Sortenbestimmung · Erwinia amylovora · europäische Steinobstvergilbung · Feuerbrand · goldgelbe Vergilbung · Vergilbungskrankheiten der rebe · Winden-glasflügelzikade · latente infektion · apfel · Mikrosatelliten Dna · Molekulare Diagnostik · Molekulare typisierung · erreger-Quantifizierung · real-time Pcr · Wurzelverwachsung · amerikanische rebzikade · Tilletiopsis spp. · Vektor · rebe · Weißer Hauch erwerbs-Obstbau (2012) 54:125-135

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Section: Molecular Typing Of 'Ca P Mali'mentioning

confidence: 99%

Section: Molecular Typing Of 'Ca P Mali'mentioning

confidence: 99%

Molecular Tools Applied to the Advancement of Fruit Growing in South Tyrol: a Review

Baric

2012

Erwerbs-Obstbau

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“…The disease commonly occurred in southern Poland in the 60' and 70' of the last century (Kamińska, Zawadzka 1970) and recently, apple proliferation was recorded in our country (Cieślińska, Kruczyńska 2011;Cieślińska et al 2012). Based on the results of the PCR-RFLP analyses the isolates of Candidatus Phytoplasma mali can be classified into three subtypes: AP, AP-1 and AT-2 (Jarausch et al 2000). Ca.…”

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confidence: 99%

Detection and molecular characterization of phytoplasmas infecting apple trees in Poland

Cieślińska

Kruczyńska

2014

Hort. Sci. (Prague)

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Cieślińska M., Kruczyńska D.E., 2014. Detection and molecular characterization of phytoplasmas infecting apple trees in Poland. Hort. Sci. (Prague), 41: 27-33.During 2010-2012, samples from 225 apple trees growing in six regions of Poland were tested for phytoplasmas. 16S rRNA gene and 16S-23S spacer region sequences were amplified from total DNAs prepared from phloem tissue of apple shoots. According to the results of PCR-RFLP and sequence analyses, apple trees were infected by Candidatus Phytoplasma mali and Ca. P. asteris. Fragments of 16S rDNA plus 16S-23S spacer region of the Ca. P. mali isolates digested with HpaII enzyme showed two restriction profiles: P-I and P-II. Multiple alignments of 16S rRNA gene fragments revealed that the isolates of Ca. P. mali shared 100% sequence identity among themselves as well as with reference strains AT and AP-15 of apple proliferation phytoplasma. The nucleotide sequence of the same region of Ca. P. asteris isolates confirmed the phylogenetic relationship with reference strains OAY (MIAY) and AY1 of aster yellows phytoplasma PCR-RFLP analysis of ribosomal protein (rpl22 and rpS3), secY, and tuf genes did not show the sequence diversity of the isolates of aster yellows phytoplasma.

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“…To elucidate the molecular diversity within this taxon, several other approaches have been employed. Typing based on restriction fragment length polymorphism analysis of ribosomal protein genes (Martini et al 2008), on sequences of nonribosomal loci including the imp, aceF, pnp, and secY genes (Danet et al 2007), and a putative nitroreductase gene (Jarausch et al 1994(Jarausch et al , 2000 revealed genetic differences in 'Ca. P. mali'.…”

mentioning

confidence: 99%

“…Two major subgroups of 'Ca. P. mali', designated AT (or AT-1) and AP, and additional subtypes have been delineated (Jarausch et al 1994(Jarausch et al , 2000Kison et al 1994;Martini et al 2008). However, the genotypes defined in these efforts were unrelated to phenotypical traits such as virulence and other pathological characteristics including symptomatology and host specificity.…”

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confidence: 99%

HflB Gene-Based Phytopathogenic Classification of ‘Candidatus Phytoplasma mali’ Strains and Evidence that Strain Composition Determines Virulence in Multiply Infected Apple Trees

Seemüller¹,

Kampmann²,

Kiss³

et al. 2011

MPMI

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Analysis of pathological and molecular data of 'Candidatus Phytoplasma mali' accessions from 27 apple trees differing considerably in symptomatology was used to molecularly characterize and classify strains of the infecting apple proliferation phytoplasma. Single-strand conformation polymorphism and sequence analysis of a variable fragment of ATP00464-type hflB gene revealed that these sources consisted of single-strain and multiple-strain accessions that occurred in similar numbers. The latter group was composed of two to five distinct strains. Analysis of cloned sequences of mild and severe single-strain accessions resulted in two groups of reads that clustered, according to their virulence, distantly in the phylogram. Based on this data, the clustering patterns of multiple-strain accession sequences indicated that nearly all of them were composed of mild and severe strains. The distinct clustering of sequences representing mild and severe strains was associated with a range of molecular markers at the nucleotide and amino acid level. Data indicate that the virulence of multiple-strain accessions is determined by the ratio of the occurring mild and severe strains in that mild accessions were characterized by the predominance of sequences representing mild strains and vice versa. There is evidence that shifts in the population and other events may occur that drastically alter virulence of multiple-strain accessions.

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Genetic variability of apple proliferation phytoplasmas as determined by PCR-RFLP and sequencing of a non-ribosomal fragment

Cited by 52 publications

References 12 publications

Molecular Tools Applied to the Advancement of Fruit Growing in South Tyrol: a Review

Molecular Tools Applied to the Advancement of Fruit Growing in South Tyrol: a Review

Detection and molecular characterization of phytoplasmas infecting apple trees in Poland

HflB Gene-Based Phytopathogenic Classification of ‘Candidatus Phytoplasma mali’ Strains and Evidence that Strain Composition Determines Virulence in Multiply Infected Apple Trees

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