Genetic variability of Aspergillus terreus from dried grapes

Abstract

RAPD was used to examine the genetic variability among five isolates of Aspergillus terreus spp. Two random primers were selected for the RAPD assay PG01-5' CAGGTGTTGC 3' and PG02-5' CTGGA-CAGAC 3' (Progen Technologies). The characterization of Aspergillus terreus species have been mostly applied on the basis of morphology, phenotype and physiology. DNA Polymorphisms are based on differences in DNA sequences and have advantages over protein polymorphisms. But morphological characterization besides molecular tools will remain a basic and powerful key in the identification of Aspergillus terreus species. The objective of the present study was to isolate the fungal contaminants from dried grapes and compare the genomic profile of the Aspergillus terreus speices isolated from the dried grapes, through RAPD analysis. In the present study with primer PG 01 four different discriminations was there among the A. terreus isolates. There was a homology of genotype between the isolates 1 & 3. And with primer PG 02 four different discriminations were there and there was a homology between 1 & 3. The predominant type was type I in primer I & II. The other isolates belonged to 2, 3 and 4. No similarity was detected for isolates 3, 4 and 5 indicating great genomic diversity of A. terreus.

Molecular ecological typing of wild type Aspergillus terreus from arid soils and screening of lovastatin production

“…Other isolates showed 0-80% similarity coefficient values ( Figure 5). These results agree with those of Lasker (2002) whose genotyped A. fumigatus isolates, and also the results of Raclasky et al (2006) and Nariasimhan and Asokan (2010).…”

Invasive <em>Aspergillus terreus</em> morphological transitions and immunoadaptations mediating antifungal resistance

“…Recently, it was shown that isolates of A. terreus specifically from dried grape showed significant genomic diversity based on random amplification of polymorphic DNA–PCR data, although morphological differences were not significant. 29 …”

Mycoflora and Biodiversity of Black Aspergilli in Vineyard Eco-Systems

“…showed that isolates of A. carbonarius and A. niger clustered into species groups, however, within species, strains displaying similar degrees of toxigenicity did not cluster together when characterized by RAPD techniques (Ilic et al, 2001(Ilic et al, , 2004. The profile of the Aspergillus terreus species isolated from dried grapes, analysed by RAPD, indicated great genomic diversity (Narasimhan & Asokan, 2010 carbonarius. In addition, no genotypical differences could be established between OTA producers and non-producers in all the species analysed.…”