Genetic variability of brazilian Alternaria spp. isolates as revealed by RAPD analysis

Tigano, Myrian S.; Al-Janabi, Salah M.; Mello, S. C. M.

doi:10.1590/s1517-83822003000200005

Cited by 8 publications

(5 citation statements)

References 6 publications

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“…Kumar et al (2008) also found no effect of origin of isolates, rather two isolates of A. solani from two different locations were closer to each other. The RAPD data presented here and other molecular studies (Roberts et al, 2000;Bock et al, 2002;Tigano et al, 2003) confirm high level of variation among isolates of a single species of Alternaria. A possible explanation for the high level of genetic diversity found among isolates of A. mali could be natural chance mutations, combined with the fact that the fungus can produce abundant numbers of spores in a relatively short period of time as reported in A. alternata (Leung et al, 1993).…”

Section: Molecular Variabilitysupporting

confidence: 79%

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

T¹,

Muzafer²,

Gh³

et al. 2013

Afr. J. Biotechnol.

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Section: Molecular Variabilitysupporting

confidence: 79%

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

T¹,

Muzafer²,

Gh³

et al. 2013

Afr. J. Biotechnol.

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“…Para análise, foram consideradas apenas as bandas fortes. Os dados foram analisados no programa NTSYS-PC 2.1, o qual construiu uma matriz de similaridade, utilizando o coeficiente de Dice (ROHLF, 1992 comerciais (CHIU et al, 1996;COLAUTO et al, 2002;KHUSH et al, 1992;RAMIREZ et al, 2001;ZHANG & MOLINA, 1995 Os isolados CS7 (Porto Alegre, RS), CS5 (Araçatuba, SP) e CS2 (Belo Horizonte, MG) formaram grupos distintos, apresentando níveis de similaridade genética abaixo de 96%, com médias de 91,3%, 88,7% e 60,6%, respectivamente. De todos eles, o isolado CS2 foi o que apresentou a maior divergência genética, em relação aos demais, apresentando similaridade variando de 59% (CS1 e CS3) a 65% (CS5) (Tabela 4).…”

Section: Foram Utilizados Nove Isolados De Agaricus Blazeiunclassified

“…Portanto, não se observou uma correlação entre similaridade genética e origem dos isolados, uma vez que, a maior distância genética (ou menor similaridade genética) TABELA 3 Matriz de Similaridade Genética (%) entre os isolados de A. bisporus e A. blazei, analisados dois a dois, de acordo com os padrões de RAPD e baseada no coeficiente de Dice. (FRAZZON et al, 2002;TEIXEIRA et al, 2004;TIGANO et al, 2003). Colauto et al (2002), caracterizando 5 isolados do cogumelo A. blazei também por análise de RAPD, verificaram que 3 deles (ABL 97/11, ABL 99/25, ABL 99/29) não apresentaram qualquer divergência, sendo caracterizados como isolados de uma mesma origem, apesar de terem sido obtidos de locais diferentes do País.…”

Section: Foram Utilizados Nove Isolados De Agaricus Blazeiunclassified

Variabilidade genética de isolados do cogumelo Agaricus blazei por meio de marcadores RAPD

et al. 2007

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RESUMOAgaricus blazei (Murrill) ss. Heinem. é um cogumelo nativo do Brasil que vem despertando a atenção de vários pesquisadores em todo o mundo, devido às suas propriedades farmacológicas e nutricionais. Este trabalho teve por objetivo, analisar a variabilidade genética de alguns isolados utilizados comercialmente, por meio de marcadores RAPD. Foram analisados nove isolados de A. blazei, provenientes de diferentes regiões do Brasil e, como controle, dois isolados de A. bisporus. Todos eles fazem parte da coleção de fungos do Laboratório de Cogumelos Comestíveis e Medicinais do DBI/UFLA. Diferentes primers aleatórios foram utilizados, gerando um total de 139 bandas polimórficas. Procedeu-se a avaliação de similaridade genética entre os isolados pelo coeficiente de Dice e análise de agrupamento pelo método UPGMA. Os resultados revelaram que dos 9 isolados de A. blazei, 6 (CS1, CS3, CS4, CS6, CS8 e CS9) apresentaram uma alta similaridade genética, sendo considerados isolados de uma mesma origem ou clones. O isolado CS2 foi o que apresentou a maior divergência genética em relação aos demais, seguido dos isolados CS5 e CS7, os quais formaram cada um, um grupo a parte, com médias de 60,6%, 88,7% e 91,3% de similaridade genética, respectivamente. Termos para indexação:Variabilidade, RAPD, Cogumelo, Agaricus blazei. ABSTRACTAgaricus blazei (Murrill) ss. Heinem. is a Brazilian native mushroom which has called the attention of several researchers all over the world due to its nutritional and pharmacological properties. The objective of this study was to evaluate the genetic variability of some isolates commercially used, using RAPD markers. Nine isolates of A. blazei were analyzed, from different regions in the country, and two isolates of the A. bisporus, which served as control group. All of them are part of the collection of mushroom of the Edible and Medicinal Mushroom Laboratory of DBI/UFLA. For RAPD analysis, different random primers were used, generating 139 polymorphic bands. The genetic similarity evaluation was proceeded between the isolates by means of Dice coefficient and grouping analysis through the UPGMA method. The results showed that from the 9 isolates of A. blazei, 6 (CS1, CS3, CS4, CS6, CS8 e CS9) have shown a high genetic similarity and these were considered isolates of the same origin or clones. The CS2 was the isolate which showed the higher genetic divergence in relation to the others, followed by the CS5 and CS7, with averages of 60.6%, 88.7% and 91.3% genetic similarity, respectively. Agaricus blazei (Murrill) ss. Heinemann é um cogumelo nativo do Brasil que se tornou mundialmente conhecido devido às suas propriedades farmacológicas e nutricionais. No Brasil, a espécie ficou conhecida popularmente como cogumelo do sol , pelo fato de crescer em campo aberto, sendo conhecido também como cogumelo piedade, cogumelo medicinal, cogumelo princesa, cogumelo-de-Deus, cogumelo da vida, cogumelo dos deuses (MIZUNO, 1995) e, mais recentemente, Champignon do Brasil (AMAZONAS, 2004). No Japão, ele é conhecido...

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“…The random amplified polymorphic DNA (RAPD) analysis allows quick assessment of genetic variability in various taxa and has been used to study inter and intraspecific variability among fungal isolates. This analysis proved to be an efficient method for detecting genetic variability of A. cassiae and A. alternata isolates occurring in Senna obtusifolia and also for distinguishing Alternaria species (Tigano et al., 2003). No information is available regarding patterns of genetic variation in A. helianthi .…”

Section: Introductionmentioning

confidence: 99%

Analysis of Cultural and Genetic Diversity in Alternaria helianthi and Determination of Pathogenic Variability Using Wild Helianthus Species

Prasad¹,

Sujatha²,

Rao³

2009

Journal of Phytopathology

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The study aims at assessment of morphological, molecular and pathogenic variability of Alternaria helianthi, incitant of leaf blight of sunflower. Morphological characteristics determined for 26 isolates of A. helianthi from India revealed variations in shape of culture, pigmentation, conidial measurements, number of septa and colony growth. The conidia of isolate Ah-7 were long, while conidia of isolate Ah-15 were short. Based on cultural characters, isolates were classified into four groups. Genetic variability of the isolates was assessed by random amplified polymorphic DNA analysis. Good polymorphism was observed and cluster analysis indicated presence of six genetically distinct groups among the isolates. The isolates Ah-1, Ah-7 and Ah-14 were genetically distinct. Resistant sources are not available in cultivated sunflower, while wild Helianthus species possess resistance to multiple stresses. We evaluated reaction of wild Helianthus species to isolates of A. helianthi. Among wild Helianthus species, H. tuberosus followed by H. occidentalis showed moderately to highly resistant reaction to all the isolates and recorded less disease incidence. The species H. argophyllus followed by H. laevigatus showed more disease incidence. The cultivated sunflower recorded susceptible reaction to most of the isolates and recorded high disease incidence. The isolates differed significantly for pathogenic reaction and were grouped into three pathogenicity groups; low, medium and high. Six isolates induced <20% disease incidence and were included in the low pathogenicity group. Majority of isolates were in the medium pathogenicity group. Six isolates i.e. Ah-9, Ah-10, Ah-18, Ah-20, Ah-24 and Ah-26 induced more than 50% disease incidence and were considered high pathogenicity group. Our results demonstrate the existence of considerable variation in resistance of Helianthus species to A. helianthi and also in morphological and genetic characters of A. helianthi isolates prevalent in India.

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Genetic variability of brazilian Alternaria spp. isolates as revealed by RAPD analysis

Cited by 8 publications

References 6 publications

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

Variabilidade genética de isolados do cogumelo Agaricus blazei por meio de marcadores RAPD

Analysis of Cultural and Genetic Diversity in Alternaria helianthi and Determination of Pathogenic Variability Using Wild Helianthus Species

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