Genetic variability of human respiratory syncytial virus group B in Panama reveals a novel genotype BA14

Ábrego, Leyda; Delfraro, Adriana; Franco, Danilo; Castillo, Juan Antonio; Castillo, Marlene; Moreno, Brechla; López‐Vergès, Sandra; Pascale, Juan Miguel; Arbiza, Juan

doi:10.1002/jmv.24838

Cited by 25 publications

(24 citation statements)

References 49 publications

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“…The alignment of the predicted amino acid sequences of strains from BA9, NA1 and ON1 genotypes confirmed the relatively high genetic variability associated with the HVR2. Strains from both of subtypes showed common changes, conserved N-glycosilation sites and subtitutions leading to loss or gain of these sites that had been previously reported [11,40,41,45,46,49,50,[54][55][56][57][58][59]. Within RSV-A strains, the number of substitutions among ON1 strains was higher compared with its NA1 counterparts.…”

Section: Discussionsupporting

confidence: 58%

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Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Sáez-López

Cristóvão

Costa

et al. 2019

Journal of Clinical Virology

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A B S T R A C TIntroduction: Respiratory syncytial virus (RSV) is associated with substantial morbidity and mortality since it is a predominant viral agent causing respiratory tract infections in infants, young children and the elderly. Considering the availability of the RSV vaccines in the coming years, molecular understanding in RSV is necessary. Objective: The objective of the present study was to describe RSV epidemiology and genotype variability in Portugal during the 2014/15-2017/18 period. Material and methods: Epidemiological data and RSV-positive samples from patients with a respiratory infection were collected through the non-sentinel and sentinel influenza surveillance system (ISS). RSV detection, subtyping in A and B, and sequencing of the second hypervariable region (HVR2) of G gene were performed by molecular methods. Phylogenetic trees were generated using the Neighbor-Joining method and p-distance model on MEGA 7.0. Results: RSV prevalence varied between the sentinel (2.5%, 97/3891) and the non-sentinel ISS (20.7%, 3138/ 16779), being higher (P < 0.0001) among children aged < 5 years. Bronchiolitis (62.9%, 183/291) and influenza-like illness (24.6%, 14/57) were associated (P < 0.0001) with RSV laboratory confirmation among children aged < 6 months and adults ≥65 years, respectively. The HVR2 was sequenced for 562 samples. RSV-A (46.4%, 261/562) and RSV-B (53.6%, 301/562) strains clustered mainly to ON1 (89.2%, 233/261) and BA9

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Section: Discussionsupporting

confidence: 58%

“…The second hypervariable region (HVR2), which carries the C-terminus of the G attachment glycoprotein, has a high degree of divergence and, thereby, it has been used as the main indicator for studies on RSV evolution [3,4]. To date, based on this region, 15 RSV-A and 29 RSV-B genotypes have been described [3,[5][6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Sáez-López

Cristóvão

Costa

et al. 2019

Journal of Clinical Virology

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“…These BA variants subsequently spread rapidly throughout the world, becoming the predominant group B genotypes. BA variants evolved rapidly into at least 16 new genotypes (BA1–BA14, CCA and CCB) [17], replacing in many countries all previous circulating RSV-B strains [50–52]. In Europe, although the BA10 genotype is frequently detected, BA9 remains the predominant genotype.…”

Section: Discussionmentioning

confidence: 99%

“…Compared with the BA10 reference strain England 583, amino acid substitutions S269F and Y287H found in Tunisian strains have already been described respectively in Japan [59]and Panama [17].…”

Section: Discussionmentioning

confidence: 99%

“…The major antigenic differences between genotypes are due to variations in the second hyper-variable region (HVR2) located at the C-terminal end of GPG. Based on HVR2 of the G gene, at least 14 genotypes (GA1–GA7 [6], SAA1 [7], NA1–NA4 [8, 9], CB-A [10] and ON1 [11]) for RSV-A, and 25 genotypes (GB1–GB4 [12, 13], URU1–2 [14], SAB1–SAB3 [13], BA1–BA14 [15–17], CBB [18], CB1 [9] and BAC [9]) for RSV-B have been described.…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of respiratory syncytial virus circulating in Tunisia between 2015 and 2018

Jerbi

Fodha

Hamida-Rebaï

et al. 2020

Journal of Medical Microbiology

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Introduction. Respiratory syncytial virus (RSV) is the most frequently identified viral agent in children with lower respiratory tract infection (LRTI). No data are available to date regarding RSV genotypes circulating in Tunisia. Aim. The aim of the present study was to investigate the genetic variability of the glycoprotein G gene in Tunisian RSV strains. Methodology. Nasopharyngeal aspirates were collected from infants hospitalized for LRTI in five Tunisian hospitals. All specimens were screened for RSV by a direct immunofluorescence assay (DIFA). To molecularly characterize Tunisian RSV strains, a phylogenetic analysis was conducted. Randomly selected positive samples were subjected to reverse transcription PCR amplifying the second hyper-variable region (HVR2) of the G gene. Results. Among a total of 1417 samples collected between 2015 and 2018, 394 (27.8 %) were positive for RSV by DIFA. Analysis of 61 randomly selected RSV strains revealed that group A RSV (78.7 %) predominated during the period of study as compared to group B RSV (21.3 %). The phylogenetic analysis showed that two genotypes of RSV-A were co-circulating: the ON1 genotype with a 72-nt duplication in HVR2 of the G gene was predominant (98.0 % of RSV-A strains), while one RSV-A strain clustered with the NA1 genotype (2.0 %). Concerning Tunisian group B RSV strains, all sequences contained a 60-nt insertion in HVR2 and a clustered BA10 genotype. Conclusion. These data suggest that RSV-A genotype ON1 and RSV-B genotype BA10, both with duplications in the G gene, were widely circulating in the Central coastal region of Tunisia between 2015 and 2018.

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Predominance of ON1 and BA9 genotypes of respiratory syncytial virus (RSV) in Bulgaria, 2016‐2018

Korsun

Angelova

Trifonova

et al. 2020

Journal of Medical Virology

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The objectives of this study were to investigate the prevalence of respiratory syncytial virus (RSV) infections in Bulgaria, to characterize the genetic diversity of the RSV strains, and to perform amino acid sequence analysis of the RSV G protein. Clinical, epidemiological data and nasopharyngeal swabs were prospectively collected from children aged less than 5 years presenting with acute respiratory infections from October 2016 to September 2018. Real‐time polymerase chain reaction for 12 respiratory viruses, and sequencing, phylogenetic, and amino acid analyses of the RSV G gene/protein were performed. Of the 875 children examined, 645 (73.7%) were positive for at least one viral respiratory pathogen. RSV was the most commonly detected virus (26.2%), followed by rhinoviruses (15%), influenza A (H3N2) (9.7%), adenoviruses (9%), bocaviruses (7.2%), human metapneumovirus (6.1%), parainfluenza viruses 1/2/3 (5.8%), influenza type B (5.5%), and A(H1N1)pdm09 (3.4%). The detection rate for RSV varied across two winter seasons (36.7% vs 20.3%). RSV‐B cases outnumbered those of the RSV‐A throughout the study period. RSV was the most common virus detected in patients with bronchiolitis (45.1%) and pneumonia (24%). Phylogenetic analysis indicated that all the sequenced RSV‐A strains belonged to the ON1 genotype and the RSV‐B strains were classified as BA9 genotype. Amino acid substitutions at 15 and 22 positions of the HVR‐2 were identified compared with the ON1 and BA prototype strains, respectively. This study revealed the leading role of RSV as a causative agent of serious respiratory illnesses in early childhood, year‐on‐year fluctuations in RSV incidence, the dominance of RSV‐B, and relatively low genetic diversity in the circulating RSV strains.

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Genetic variability of human respiratory syncytial virus group B in Panama reveals a novel genotype BA14

Cited by 25 publications

References 49 publications

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Epidemiology and genetic variability of respiratory syncytial virus in Portugal, 2014–2018

Molecular characterization of respiratory syncytial virus circulating in Tunisia between 2015 and 2018

Predominance of ON1 and BA9 genotypes of respiratory syncytial virus (RSV) in Bulgaria, 2016‐2018

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