Genetic Variant of SARS-CoV-2 Isolates in Indonesia: Spike Glycoprotein Gene

Ansori, Arif Nur Muhammad; Kharisma, Viol Dhea; Muttaqin, Sahal Sabilil; Parikesit, Arli Aditya

doi:10.22207/jpam.14.spl1.35

Cited by 35 publications

(20 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly nucleotide substitutions among SARS-CoV-2 genomes tended to switch between bat RaTG13 coronavirus sequence and Wuhan-Hu-1 genome, indicating the higher genetic instability or tolerance of mutations on those sites or suggesting that major viral strains might exist between Wuhan-Hu-1 and RaTG13 coronavirus 31 Lokman [ 47 ] Research article Bangladesh COVID-19 patients N-terminal domain (NTD) and receptor-binding domain (RBD) and angiotensin-converting enzyme 2 (ACE2) Spike glycoprotein is one of the major targets to be explored because of its role during coronaviruses' entry into host cells. Variations located at the N-terminal domain (NTD) and the receptor-binding domain (RBD) might alter the interaction of S protein with the host receptor angiotensin-converting enzyme 2 (ACE2) 32 Muhammad Ansari [ 48 ] Research article Indonesia COVID-19 patients SARS-CoV-2 spike glycoprotein gene sequences Therefore, the coronavirus spike glycoprotein mediates membrane fusion and viral entry into host cells and is the primary target for many neutralizing antibodies. The spike glycoprotein has two domains, S1 and S2, where S1 is responsible for binding the virion to ACE2 on the host cell membrane 21.…”

Section: Resultsmentioning

confidence: 99%

Characterization of SARS-CoV-2 different variants and related morbidity and mortality: a systematic review

et al. 2021

View full text Add to dashboard Cite

Introduction Coronavirus Disease-2019 (SARS-CoV-2) started its devastating trajectory into a global pandemic in Wuhan, China, in December 2019. Ever since, several variants of SARS-CoV-2 have been identified. In the present review, we aimed to characterize the different variants of SARS-CoV-2 and explore the related morbidity and mortality. Methods A systematic review including the current evidence related to different variants of SARS-CoV-2 and the related morbidity and mortality was conducted through a systematic search utilizing the keywords in the online databases including Scopus, PubMed, Web of Science, and Science Direct; we retrieved all related papers and reports published in English from December 2019 to September 2020. Results A review of identified articles has shown three main genomic variants, including type A, type B, and type C. we also identified three clades including S, V, and G. Studies have demonstrated that the C14408T and A23403G alterations in the Nsp12 and S proteins are the most prominent alterations in the world, leading to life-threatening mutations.The spike D614G amino acid change has become the most common variant since December 2019. From missense mutations found from Gujarat SARS-CoV-2 genomes, C28854T, deleterious mutation in the nucleocapsid (N) gene was significantly associated with patients' mortality. The other significant deleterious variant (G25563T) is found in patients located in Orf3a and has a potential role in viral pathogenesis. Conclusion Overall, researchers identified several SARS-CoV-2 variants changing clinical manifestations and increasing the transmissibility, morbidity, and mortality of COVID-19. This should be considered in current practice and interventions to combat the pandemic and prevent related morbidity and mortality.

show abstract

Section: Resultsmentioning

confidence: 99%

Characterization of SARS-CoV-2 different variants and related morbidity and mortality: a systematic review

et al. 2021

View full text Add to dashboard Cite

show abstract

“…17 Articles were searched for keywords COVID-19 and SARS-CoV-2. 19.940 articles were obtained (849 from Elsevier, 9,547 from PMC, and 830 from Springer). The inclusion criteria we used were articles that discussed human immune responses to SARS-CoV-2 infection (based on previous SARS-CoV infections), which were fully accessible.…”

Section: Review Methodsmentioning

confidence: 99%

“…7 There are no significant differences in the SARS-CoV-2 glycoprotein spike gene sequence found in Indonesia and the Wuhan-Hu-1 isolate from China. 19 SARS-CoV-2 has 96.2% genetic similarity with bat Coronavirus, 1 and have a 91% genome similarity to the anteater Coronavirus. 15 The structure of SASR-CoV-2 also has similarities with other SARS viruses.…”

Section: How To Sars-cov-2 Infect a Human (Based On Sars-cov)mentioning

confidence: 99%

See 1 more Smart Citation

Human immune response to SARS-CoV-2 infection

2020

View full text Add to dashboard Cite

COVID-19 is an acute respiratory infection caused by a new type of Coronavirus, SARS-CoV-2, which first appeared in Wuhan, China in December 2019. COVID-19 then became a pandemic in various countries in early 2020. In this article it contains review that discusses the immune response in humans due to SARS-CoV-2 infection, using the narrative literature review method, a total of 36 articles (6 from Elsevier, 24 from PMC, and 6 from Springer). It is known that the pathogenesis of COVID-19 and the manufacture of drugs and vaccines are still under investigation, but in infected patients, innate immune responses in the form of alveolar macrophages, dendritic cells, airway epithelial cells, congenital lymphocytes, and neutrophils work together in the fight against infection. Next comes the adaptive immune response in the form of antibodies (immunoglobulins) which help in fighting infections due to SARS-CoV-2. These immune responses include increasing levels of cytokines, coagulation parameters, C-reactive protein, neutrophils, and decreasing total lymphocytes. It is also known that COVID-19 patients with severe disease often experience higher total antibody, IgM responses, and IgG responses than COVID-19 patients without congenital disease. IgG antibodies are present in the serum, so the serum in COVID-19 patients who have recovered can be used for therapy in COVID-19 patients who have not healed, as long as the drug and vaccine are under investigation.

show abstract

“…[2,3]. The Coronavirus family has been known to mutate in several locations worldwide, including Indonesia [4]. According to a recent study, there are four distinct kinds of COVID-19: Alpha (B.1.17), Beta (B.1.351), Gamma (P.1/B.1.352), and Delta Coronavirus (B.1.617.2) [5,6].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Analysis of the Antioxidant Compounds of Baper Tea and Its Potential as an Immunomodulatory agent and Candidate for Standardized Herbal Medicine

Adnyana

Sudaryati²

2022

Trends Sci

View full text Add to dashboard Cite

The antioxidant content of Baper tea was determined using phytochemical testing, and the potential of these contents as immunomodulatory agents and candidates for standardized herbal medications was investigated. A randomized design was used in this experimental study. Phytochemical assays are used to determine the antioxidant content of Baper tea, whereas organoleptic studies are performed using hedonic tests. A literature review was used to assess the content's potential as an immunomodulatory agent and candidate for standardized herbal medicine. The study lasted four months at the Biology and National Food and Drug Agency Laboratory in Bali Province. Data analysis is based on descriptive methods. Baper tea is prepared in a 2.1.1 ratio. According to the hedonic test results, 41 panelists (87.23 %) prefer Baper tea products based on their color assessment, flavor, aroma, community reception, and acceptability. The matrix and security test revealed a water content of 4.43 %, an IC50 of 24.27 ppm, α-glucosidase inhibitors of 12.18 ppm, a total phenolic content of 28.00 %, and a total flavonoid content of 15.57 %. Baper tea raw materials contain components that have the potential to act as immunomodulatory agents and candidates for standardized herbal medications based on local wisdom. HIGHLIGHTS There are very few standardized herbal medicinal products that are efficacious as immunomodulators in the market Shallot skin (Allium Cepa), morel berry leaves (Physalis angulata), and small gooseberry leaves (Phyllanthus urinaria) packaged in Baper Tea preparations contain bioactive compounds that have the potential to be used as immunomodulatory agents and candidate for standardized herbal medicine Baper tea contains very active antioxidant compounds at a formulation ratio of 2:1:1. With panelists' acceptance of Baper Tea reaching 87.23 % GRAPHICAL ABSTRACT

show abstract

Genetic Variant of SARS-CoV-2 Isolates in Indonesia: Spike Glycoprotein Gene

Cited by 35 publications

References 28 publications

Characterization of SARS-CoV-2 different variants and related morbidity and mortality: a systematic review

Characterization of SARS-CoV-2 different variants and related morbidity and mortality: a systematic review

Human immune response to SARS-CoV-2 infection

Phytochemical Analysis of the Antioxidant Compounds of Baper Tea and Its Potential as an Immunomodulatory agent and Candidate for Standardized Herbal Medicine

Contact Info

Product

Resources

About