Genetic variation among Saudi tomato (Solanum lycopersicum L.) landraces studied using SDS-PAGE and SRAP markers

Shaye, Najla A. Al; Migdadi, Hussein M.; Charbaji, Asma; Alsayegh, Shatha; Daoud, Shaza; AL-Anazi, Wala; Alghamdi, Salem S.

doi:10.1016/j.sjbs.2018.04.014

Cited by 17 publications

(11 citation statements)

References 52 publications

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“…The two marker systems were successful in characterizing the four tomato varieties by unique positive and/or negative markers (Table 6). The present finding is consistent with the earlier report of Comlekcioglu et al (2010); Aguilera et al (2011); Mane et al (2013), Henareh et al (2016; Al Shaye et al (2018) and Kiani and Siahchehreh (2018).…”

Section: Disscussionsupporting

confidence: 94%

Agro - Morphological and Molecular Analysis of Somaclonal Variation Among Regenerated Plants from Tomato (Lycopersicon esculentum Mill.) Varieties

El-Fattah

Haridy

2019

Journal of Agricultural Chemistry and Biotechnology

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Genetic variations in tissue-culture tomato plants has been examined by agro-morphological evaluation and molecular markers analysis. Four tomato varieties were used as donor parents, two types of explants (Cotyledone and hypocotyle) and two combinations of auxin and cytokinin with different concentrations were used for the first time to study the effect of genotype, type of explants and growth regulators on callus induction and plant regeneration in tomato. Significant differences among tomato varieties were observed in all tissue culture studied traits (percentage of callus induction, regeneration rate, number of regenerated plants and number roots per explant). These differences were depending on genotype, explant type, type and concentration of growth regulators. The best regeneration medium from each growth regulator combination distinct used for establishment of regenerated plants. Regenerated plants from each combination of growth regulators which exhibited wide variations compared with its donor parent were selected and subjected to somaclonal variation analysis using molecular markers and agro-morphological traits. Two molecular marker systems, ISSR (inter simple sequence repeat) and SRAP (sequence related amplified polymorphism) were used to analysis of somaclonal variation. These markers revealed polymorphism showing distinct different banding patterns in all somaclones, which were prominent in their differences from the donor parents. To confirm stability of these variation, regenerated plants (R 0 ) from donor parents were transferred to the greenhouse and the first generation of these somaclones (R 1 ) as well as their donor parents were evaluated for plant height, number of branches per plant, No. of inflorescences per plant, No. of flowers per inflorescence, No. of flowers per plant, number of fruits per plant and yield per plant. Generally, the results revealed that some somaclones exceeded their donor parents in one or more traits.

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Section: Disscussionsupporting

confidence: 94%

Agro - Morphological and Molecular Analysis of Somaclonal Variation Among Regenerated Plants from Tomato (Lycopersicon esculentum Mill.) Varieties

El-Fattah

Haridy

2019

Journal of Agricultural Chemistry and Biotechnology

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“…Landraces represent an important alternative, as they constitute a reservoir of genetic diversity, with their important abiotic stress tolerance and high fruit quality. Tomato landraces contain valuable alleles uncommon in highlight germplasms [2]; therefore, these local populations represent a valuable resource of genetic traits that can be used in breeding programs for the improvement of the crop [3].…”

Section: Introductionmentioning

confidence: 99%

The Antioxidant Profile Evaluation of Some Tomato Landraces with Soil Salinity Tolerance Correlated with High Nutraceuticaland Functional Value

et al. 2020

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Romania has a wide variety of local landraces and heirloom genotypes. Our study aims to assess the performance of twenty halotolerant tomato landraces, collected from areas with medium and high levels of soil salinity, in terms ofthe accumulation of antioxidant compounds in fruits and to cluster them according to their nutraceutical components. The tomatoes used in the study were harvested once they had attained full ripeness and then analyzed for lycopene (Lyc), ascorbic acid content (AsA), total phenolic content (TPC), and total antioxidant capacity (TAC). The results revealed major differences between genotypes in terms of nutraceutical values. According to principal component analysis, the tomato landraces were grouped into five clusters, characterized by different proportions of compounds with antioxidant activity. The high/moderate nutritional values of Lyc, TAC, TPC, and AsA were obtained from varieties taken from local lands with high soil salinity, over 6.5 dS m−1. These findings support the idea that metabolites and secondary antioxidants are involved in the process of stress adaptation, thereby increasing salinity tolerance in tomatoes. Our results show that there are tomato landraces with a tolerance of adaptation to conditions of high soil salinity and provide information on their ability to synthesize molecules with antioxidant functions that protect plants against oxidative damage.

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“…Al Shaye et al [110] evaluated Saudi tomato landraces with SDS-PAGE and SRAP markers. It was shown that almost all of the landraces with the same origin were grouped in the same cluster emphasizing the usefulness of these markers in future breeding programs [110].…”

Section: The Evaluation Of the Tomatoes Landraces Genetic Diversity Bmentioning

confidence: 99%

Vegetable Landraces: The “Gene Banks” for Traditional Farmers and Future Breeding Programs

Sumalan¹,

Ciulca²,

Şumălan³

et al. 2021

Landraces - Traditional Variety and Natural Breed

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Crop diversity of vegetable species is threatened by the current homogenization of agricultural production systems due to specialization of plant breeders and increasing globalization in the seed sector. With the onset of modern agriculture, most traditional vegetable cultivars were replaced by highly productive and often genetically uniform commercial breeds and hybrids. This led to the loss of landraces, especially in countries with a super-intensive agriculture. The agricultural biodiversity erosion represents a huge risk for food safety and security. Vegetable landraces are associated with the cultural heritage of their place of origin being adapted to local agro-ecological areas and are more resilient to environmental stress than commercial cultivars. The chapter aim to highlight the importance of keeping and using vegetable landraces as valuable sources of genes for traditional farmers, but also for future breeding processes. We analyze the historical role of landraces, genetic diversity, high physiological adaptability to specific local conditions in association with traditional farming systems, as well as the breeding perspectives and evaluation of genetic diversity based on molecular markers.

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Genetic variation among Saudi tomato (Solanum lycopersicum L.) landraces studied using SDS-PAGE and SRAP markers

Cited by 17 publications

References 52 publications

Agro - Morphological and Molecular Analysis of Somaclonal Variation Among Regenerated Plants from Tomato (Lycopersicon esculentum Mill.) Varieties

Agro - Morphological and Molecular Analysis of Somaclonal Variation Among Regenerated Plants from Tomato (Lycopersicon esculentum Mill.) Varieties

The Antioxidant Profile Evaluation of Some Tomato Landraces with Soil Salinity Tolerance Correlated with High Nutraceuticaland Functional Value

Vegetable Landraces: The “Gene Banks” for Traditional Farmers and Future Breeding Programs

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