Genetic variation in the dietary sucrose modulation of enzyme activities in<i>Drosophila melanogaster</i>

Geer, Billy W.; Laurie-Ahlberg, C. C.

doi:10.1017/s0016672300026094

Cited by 31 publications

(17 citation statements)

References 20 publications

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“…Geer et a!. (1979a, b) and Geer and Laurie-Ahlberg (1984) have shown that adaptation to dietary sucrose in D. melanogaster increases activity in the enzyme G6PD. As shown in fig.…”

Section: Resultsmentioning

confidence: 99%

The role of glucose-6-phosphate dehydrogenase in the evolution of longevity in Drosophila melanogaster

et al. 1990

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Different polymorphic elements of the enzyme glucose-6-phosphate dehydrogenase (G6PD) are favoured under selection for long versus short life span. Replicate independently selected populations of short-lived individuals exhibit a more rapidly migrating and less actively staining allozyme, while long-lived populations have a slower migrating and more active allozyme. These correspond to the common ZwA and ZwB variants of the G6PD locus Zw. In vitro measurements show G6PD activity varies with allozymes and life span. Long-lived males have 64 per cent greater activity in G6PD, while females of long-lived strains are 108 per cent higher than those of short-lived strains. Previous studies of these strains have repeatedly demonstrated additivity of life span in F1 crosses. Activity of G6PD in reciprocal F1 populations is additive and intermediate between parents.

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“…Geer et a!. (1979a, b) and Geer and Laurie-Ahlberg (1984) have shown that adaptation to dietary sucrose in D. melanogaster increases activity in the enzyme G6PD. As shown in fig.…”

Section: Resultsmentioning

confidence: 99%

The role of glucose-6-phosphate dehydrogenase in the evolution of longevity in Drosophila melanogaster

et al. 1990

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“…This explanation is, however, likely an oversimplification. Apparently compensatory changes in enzyme activity have been documented between the oxidative pentose shunt enzymes and MEN; extreme reduction in MEN activity is associated with an increase in G6PD and 6PGD activity Geer and Laurie-Ahlberg 1984). However, no such compensation has been found between IDH and MEN; reduction in MEN is associated with reduction, not increase, in IDH activity Geer and Laurie-Ahlberg 1984).…”

mentioning

confidence: 99%

“…Apparently compensatory changes in enzyme activity have been documented between the oxidative pentose shunt enzymes and MEN; extreme reduction in MEN activity is associated with an increase in G6PD and 6PGD activity Geer and Laurie-Ahlberg 1984). However, no such compensation has been found between IDH and MEN; reduction in MEN is associated with reduction, not increase, in IDH activity Geer and Laurie-Ahlberg 1984). Similarly, an increase in dietary carbohydrate increases G6PD, 6PGD, and MEN activity, but decreases IDH activity (Geer et al 1978;Geer and Laurie-Ahlberg 1984).…”

mentioning

confidence: 99%

“…However, no such compensation has been found between IDH and MEN; reduction in MEN is associated with reduction, not increase, in IDH activity Geer and Laurie-Ahlberg 1984). Similarly, an increase in dietary carbohydrate increases G6PD, 6PGD, and MEN activity, but decreases IDH activity (Geer et al 1978;Geer and Laurie-Ahlberg 1984). Finally, reduction in IDH activity was associated with an increase in G6PD and 6PGD, but a decrease in MEN activity (Bentley et al 1983).…”

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confidence: 99%

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Natural and Synthetic Alleles Provide Complementary Insights Into the Nature of Selection Acting on theMenPolymorphism ofDrosophila melanogaster

Merritt

Duvernell²,

Eanes

2005

Genetics

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Two malic enzyme alleles, Men113A and Men 113G, occur at approximately equal frequency in North American populations of Drosophila melanogaster, while only Men 113A occurs in African populations. We investigated the population genetics, biochemical characteristics, and selective potential of these alleles. Comparable levels of nucleotide polymorphism in both alleles suggest that the Men 113G allele is not recently derived, but we find no evidence in the DNA sequence data for selection maintaining the polymorphism. Interestingly, the alleles differ in both V max and K m for the substrate malate. Triglyceride concentration and isocitrate dehydrogenase (IDH) and glucose-6-phosphate dehydrogenase (G6PD) activities are negatively correlated with the in vivo activities of the Men alleles. We examined the causality of the observed correlations using P-element excision-derived knockout alleles of the Men gene and found significant changes in the maximum activities of both IDH and G6PD, but not in triglyceride concentration, suggesting compensatory interactions between MEN, IDH, and G6PD. Additionally, we found significantly higher than expected levels of MEN activity in knockout heterozygotes, which we attribute to transvection effects. The distinct differences in biochemistry and physiology between the naturally occurring alleles and between the engineered alleles suggest the potential for selection on the Men locus. SIGNIFICANT progress has been made in characterizing population variability at the level of DNA sequences, but the central challenge of elucidating rules that connect genotype and phenotype remains. In many cases, genotype may give rise to phenotype through interactions among multiple genes and metabolite intermediates. Presumably, these interactions are at least partly the product of natural selection. The challenge, however, is not to simply infer the presence of selection, but to elucidate its action and distribution across such multiple gene networks. In this study we begin an analysis of the variation and functional interactions among three oxidative enzymes known to be the primary source of the reduced form of the metabolic cofactor NADP.A single amino acid polymorphism was identified in cytosolic malic enzyme (Men) as part of a study of geographic variation of single-nucleotide polymorphisms associated with the genes of central metabolic enzymes in Drosophila melanogaster (Sezgin et al. 2004). The polymorphism is an alanine-to-glycine substitution at amino acid 113 and the two alleles defined by the amino acid substitution occur in approximately equal frequency in all 10 North American populations examined. Allele frequency clines were detected at a number of other metabolic genes, but these Men alleles and associated haplotypes showed no clinal changes with latitude. While the presence of clinal change in allele frequency can be interpreted as evidence for selection on a locus, the lack of such a pattern does not indicate that a polymorphism is necessarily neutral. Here we examine other as...

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“…Hence the major antioxidant systems are dependent on the availability of NADPH that is principally produced by G 6 PD. Adaptation to dietary sucrose in D. melanogaster increases activity in the enzyme [54,55]. G 6 PD activity has studied in different genetic stocks of D. melanogaster to correlate lifespan [56].…”

Section: G 6 Pd Assaymentioning

confidence: 99%

Asparagus racemosus extract increases the life span in Drosophila melanogaster

Kumar¹,

Prasanna²,

Ashadevi³

2015

J App Biol Biotech

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Free radicals and oxidative stress are recognized as important factors in the biology of aging as well as in many age-related diseases. Dietary antioxidant plays a potential role in the reduction of age-related diseases. Many medicinal plants are rich source of antioxidants and promote longevity. Drosophila is a suitable animal model to study ageing and oxidative study. This study evaluated the antioxidant property of Asparagus racemosus on longevity and G6PD activity in Drosophila melanogaster. The analyses were carried out in stress and non stress conditions. Longevity increased almost by 29% at low concentration and 41% at high concentration in extract supplemented flies. G6PD level was significantly increased in extract supplemented larval stages than adults. The antioxidant property of Asparagus racemosus increases the larval G6PD activity thus; it extends the lifespan in adults.Abbreviation A. racemosus Asparagus racemosus, ACR Acrylamide, OS Oxidative stress, G6PD Glucose 6 Phosphate Dehydrogenase.

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Genetic variation in the dietary sucrose modulation of enzyme activities inDrosophila melanogaster

Cited by 31 publications

References 20 publications

The role of glucose-6-phosphate dehydrogenase in the evolution of longevity in Drosophila melanogaster

The role of glucose-6-phosphate dehydrogenase in the evolution of longevity in Drosophila melanogaster

Natural and Synthetic Alleles Provide Complementary Insights Into the Nature of Selection Acting on theMenPolymorphism ofDrosophila melanogaster

Asparagus racemosus extract increases the life span in Drosophila melanogaster

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