2022
DOI: 10.1007/s13353-022-00731-x
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Genetic variation of Cerastium alpinum L. from Babia Góra, a critically endangered species in Poland

Abstract: Babia Góra massif is the only site of occurrence of the Cerastium alpinum L. in Poland, an arctic-alpine perennial plant with a wide distribution in North America, northwestern Asia, and Europe. To determine whether the isolated Polish populations are genetically distinct, we have performed an evaluation of C. alpinum from Babia Góra with the use of iPBS markers. A total number of 133 individuals of C. alpinum from seven populations representing four localizations of the species were analyzed, i.e., from Babia… Show more

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Cited by 3 publications
(2 citation statements)
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“…Based on the results of initial tests of 34 PBS primers (Kalendar et al 2010), a set of seven primers that gave polymorphic, clearly identifiable, and repeatable bands were selected for further analyses (Table 1). The initial tests and optimalisation of PCR conditions for studied Avena species were conducted according to the procedure described in Milarska et al (2022). The reproducibility of band profiles for each of the selected PBS primers was verified.…”
Section: Dna Extraction and Ipbs Genotypingmentioning
confidence: 99%
“…Based on the results of initial tests of 34 PBS primers (Kalendar et al 2010), a set of seven primers that gave polymorphic, clearly identifiable, and repeatable bands were selected for further analyses (Table 1). The initial tests and optimalisation of PCR conditions for studied Avena species were conducted according to the procedure described in Milarska et al (2022). The reproducibility of band profiles for each of the selected PBS primers was verified.…”
Section: Dna Extraction and Ipbs Genotypingmentioning
confidence: 99%
“…The current state of knowledge about the genetic diversity of the genus Cerastium is based on a rather limited number of studies. Genetic investigations employed analysis of isoenzymatic polymorphism 4 , 5 and different molecular markers like RAPD and SCAR 6 , AFLP 7 , 8 and iPBS 9 . Apart from traditional genetic diversity studies, there are also papers that focused on the role of hybridization and introgression events in the evolution of the genus Cerastium 10 – 12 .…”
Section: Introductionmentioning
confidence: 99%