Genetic variation of Croton stellatopilosus Ohba based on non-coding DNA sequences of ITS, trnK and trnL-F regions

Abstract

Croton stellatopilosus Ohba (Plau-noi), a well-known Thai medicinal plant, was investigated for its genetic variation by analyzing three DNA regions, one nuclear internal transcribed spacer (ITS) region and two chloroplast trnL-F intergenic spacer and trnK intron regions. The results of ITS sequencing from 30 leaf samples showed that there were two major genotypes of C. stellatopilosus which were designated as STEL Type A and B. In addition, various nucleotide additive sequences which had presumably arisen from these two groups were also found. These so-called "putative hybrids", interestingly, displayed trnK intron sequences identical to the STEL Type B but different from the Type A. For the trnL-F region, all the 30 samples showed identical sequences. Thus, it was suggested that in the hybridization of C. stellatopilosus, the Type A genotype acts as paternal parent whereas the Type B genotype acts as maternal parent. In addition, all C. stellatopilosus samples were analyzed for their plaunotol content using TLC densitometry. We found that the Type A genotype, hybrid group and Type B genotype had plaunotol content in the ranges 0.209-0.492, 0.319-0.896 and 0.442-1.000% (w/w) dry weight, respectively. The results indicated that there is a correlation between the plaunotol contents and non-coding DNA sequences of ITS, trnK and trnL-F regions of C. stellatopilosus.

Genetic diversity and population structure of Croton urucurana Baill. (Euphorbiaceae) in Central Brazil by ISSR markers

“…Arg. (Scaldaferri et al 2013(Scaldaferri et al , 2014Silva et al 2018), C. stellatopilosus Ohba (Rinthong et al 2011) andC. tetradenius Baill.…”

Refining DNA Barcoding Coupled High Resolution Melting for Discrimination of 12 Closely Related Croton Species

“…HRM can detect differences among samples of as little as one base pair, but in order to allow for intra-specific and geographical variations within species, an arbitrary confidence interval cut-off of 90% was chosen based on previous molecular studies. The sequence variation in ITS1 among thirty samples of Croton stellatopilosus collected from different parts of Thailand shows that these can be divided into three groups based on the occurrence of indels [ 52 ].…”

Transferability of microsatellite loci from Croton floribundus Spreng. to Croton urucurana Baill. (Euphorbiaceae)

“…Because of this great medicinal importance, genetic studies are essential to develop conservation strategies and also products (Alves et al, 2012). For Croton genus there are some research related to genetic diversity (Angelo et al, 2005;Silvestrini et al, 2015;Rinthong et al, 2011;Scaldaferri, 2013Scaldaferri, , 2014Oliveira et al, 2016;Almeida-Pereira et al, 2017;Silva et al, 2018), however, molecular information are still scarce; for species C. urucurana they are almost non-existent and it is necessary to advance in the genetic diversity characterization of this species.…”