Genetic variation of Leptospira isolated from rats catched in Yogyakarta Indonesia

Sumanta, Hadi; Wibawa, Tri; Hadisusanto, Suwarno; Nuryati, Anik; Kusnanto, Hari

doi:10.1016/j.apjtm.2015.07.029

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…Study by Sumanta (2015), mentioned of 99 rat kidneys examined for Leptospira DNA, 25 came out positive using qPCR and 6 was positively confirmed for pathogenic Leptospira using standard PCR. Pathogenic Leptospira examination using LipL 32 gene target were already performed in water sample from nine villages in Demak district and resulted 46.7% positively containing pathogenic Leptospira DNA (Widiastuti, 2015).…”

Section: Resultsmentioning

confidence: 96%

Leptospirosis: New Emerging Disease in Sukoharjo District

Marbawati¹,

Pramestuti²

2017

Kemas

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Leptospirosis in Sukoharjo was discovered in 2014. Examination with RDT (Rapid Diagnostic Test) leptotek supported by clinical symptoms showed 6 positive cases of Leptospira, and until March 2015 one person was found to be Leptospira positive. The aim of this study was to identify rats as the main reservoir of leptospirosis, calculate the catching rate of rats and to detect the presence of pathogenic Leptospira in rats. This study was a cross-sectional survey, and was conducted in Pabelan village Kartasura Sukoharjo on May 2015. Polymerase Chain Reaction (PCR) assay was conducted in Bacteriology Laboratory Balai Litbang P2B2 Banjarnegara to detect leptospira in the kidney of the rats. Data were analyzed descriptively. Results of rats and shrew catching obtained Rattus tanezumi, Rattus norvegicus and Suncus murinus. The species most commonly found was balanced between R. tanezumi and S.murinus (46%). The trap success rate inside and outside the house are 1.50% and 5%, respectively. Result of laboratory test showed from 13 rats kidneys, two kidneys were found to be Leptospira positive and was from R.tanezumi and R.norvegicus.

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Section: Resultsmentioning

confidence: 96%

Leptospirosis: New Emerging Disease in Sukoharjo District

Marbawati¹,

Pramestuti²

2017

Kemas

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“…Rodents were first discovered as reservoirs and natural hosts capable of sustaining the presence of Leptospira without exhibiting symptoms. Several research findings revealed that several types of rodents tested positive for Leptospira bacteria in various areas [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Infestation of Leptospira in Rodents at the Perimeter Area in “Pelabuhan Ratu” Port, Sukabumi District

Santi¹,

Astuti²,

Yuliasih³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Leptospirosis is a neglected zoonotic disease with global health implications. A significant impact of Leptospirosis happens in tropical regions, including Indonesia. Rodents play an essential role in the spread of Leptospirosis due to their rapid population growth and proximity to humans. This study aims to determine the rodent population density and the presence of leptospira bacterial infestations in the proximity of the "Pelabuhan Ratu" Port, Sukabumi district. This study's survey was conducted monthly for six months (January, February, March, May, June, and August) in 2021 and for three months (January, March, and April) in 2022. The study's survey was held for 15-16 h; a single trap was used to catch rodents. One hundred traps are placed on the port's perimeter inside and outside the building. Leptospira bacteria were detected using PCR in rodents captured between January and March 2022. According to the survey results, 120 rodents were captured, and the sex proportions of rodents collected in the survey were nearly equal. Rattus norvegicus, Rattus tanezumi, and Bandicota indica were all captured. The average population of dominant rodents in the 2021 survey was R. tanezumi (4.3), while R. norvegicus (17.3) was mainly found in the 2022 survey. The average success rate of traps in 2021-2022 was 7.83 and 24.3, respectively. Rodents were found to have Leptospira bacteria (29.09%). The density of rodents in the proximity of the "Pelabuhan Ratu" port is considered high (> 1). Leptospira is only found in R. norvegicus caught at the fish market in this study site. This information is critical for early detection in the effort to raise public awareness of the occurrence of Leptospirosis transmission. It is necessary to improve communication, information, and education to traders and visitors in maintaining personal and environmental hygiene and cross-sectoral collaboration to improve environmental sanitation, particularly in public spaces.

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“…Rodents have been reported as the primary reservoirs of pathogenic leptospires, reason why there are numerous investigations worldwide with variable infection rates (Agudelo-Flórez et al 2009, Sumanta et al 2015. This characteristic is due to the ability of Leptospira spp.…”

Section: Inroductionmentioning

confidence: 99%

Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México

Torres–Castro¹,

Cruz-Camargo²,

Medina-Pinto³

et al. 2017

Austral j. vet. sci.

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ABSTRACT. Leptospira spp. is a spirochete bacteria, causal agent of leptospirosis, zoonotic disease endemic in México that represents a serious public health and veterinary problem. Rodents are recognised as the most important reservoirs of this bacteria, which is transmitted mainly through direct or indirect contact with the Leptospira spp. excreted in the urine of infected individuals. The aim of this study was to evaluate the circulation of Leptospira spp. in urine samples of wild and synanthropic rodents from Yucatán, México. Eighty-four rodents were captured in the community of Cenotillo, Yucatán. Twenty-six urine samples were collected from the bladder and were used in the total DNA extraction. The identification of Leptospira spp. was intended through the polymerase chain reaction test in its endpoint variant. No evidence of Leptospira spp. was found in the urine samples. It is necessary to use other tissues for the identification of Leptospira spp., before concluding that the rodents used in the present study are not reservoirs of this bacteria.

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Genetic variation of Leptospira isolated from rats catched in Yogyakarta Indonesia

Abstract: It can be considered that rats are the most important vector and reservoir of Leptospira.

Cited by 7 publications

References 25 publications

Leptospirosis: New Emerging Disease in Sukoharjo District

Leptospirosis: New Emerging Disease in Sukoharjo District

Infestation of Leptospira in Rodents at the Perimeter Area in “Pelabuhan Ratu” Port, Sukabumi District

Absence of molecular evidence of Leptospira spp. in urine samples collected from rodents captured in Yucatán, México

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