Genetic Variation of Sago Palm (Metroxylonsagu Rottb.) Progenies with Natural Pollination by Using RAPD Markers

Abstract

Sago palm is flowering and fruiting just once in their life cycle. Sago palms that grow naturally and semi cultivated were generally occurred natural pollination to form fruits and seeds, if not cut down to take the starch contained in their trunk. Sago palm pollination may occur as self-pollinated and cross-pollinated. If cross-pollinated was occurred in the pollination process, it will be varied of their progenies. This study aims to reveal the genetic variation of sago palms progenies with naturally pollinated process. The research method is to collect seeds from one parent trees that have produced ripe fruit. Fruit seeds germinated to be made and tested genetic variation using RAPD markers. Isolation of DNA is done by using the fresh young leaves. DNA amplification is done by using RAPD primers. The results showed that the progenies derived from naturally pollinated of sago palms were genetically varied based on RAPD markers and also varied based on morphological phenotypic. Variations occurred in the progenies of sago palm indicated that the sago palms were estimated cross-pollinated naturally, as a result fruits and seeds with genetically differences.

Morphology, growth and genetic variations of sago palm (Metroxylon sagu) seedlings derived from seeds

“…This opinion was consistent with Lengkong and Runtunuwu (2005) who reported that the genetic diversity among West African Tall coconut seedlings was quite high, the diversity was estimated due to seeds derived from open pollination so that individual coconut has a different genotype from one to another. In the related study, it was reported that the seedlings of sago palm derived from seeds were differentiated (Abbas et al 2017). This phenomenon shows that generally, sago palm in the natural condition was the result of cross-pollination.…”

First high-quality genome assembly data of sago palm (Metroxylon sagu Rottboll)

“…In recent years, the sago palm has been placed in the limelight of agricultural molecular research subsequent to the whole chloroplast genome sequencing, orgnanellar genome copy number investigation, genome survey, genome size estimation, population genetics, transcriptomics as well as proteomics (Wee & Roslan, 2012;Abbas et al, 2017;Lim et al, 2020a;Lim et al, 2020b;Nisar & Hussain, 2020;Lim et al, 2021). The origin of life is often associated with the central dogma of molecular biology and genes are deemed the building blocks of life.…”

Characterization of sago tree parts from Sentani, Papua, Indonesia for biomass energy utilization

“…A single sago plant can yield approximately 200–250 kg of wet sago each year, which equates to 25 tons of dry starch per hectare. This starch contains 84.7 g of carbohydrates per 100 g [ 8 , 13 ]. Sago starch extraction employs various methods, ranging from traditional to semi-modern and modern techniques.…”