1996
DOI: 10.1002/j.1460-2075.1996.tb00330.x
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Genetic variation of the poliovirus genome with two VPg coding units.

Abstract: Amongst the picornaviruses, poliovirus encodes a single copy of the genome‐linked protein, VPg wheras foot‐and‐mouth disease virus uniquely encodes three copies of VPg. We have previously shown that a genetically engineered poliovirus genome containing two tandemly arranged VPgs is quasi‐infectious (qi) that, upon genome replication, inadvertently deleted one complete VPg sequence. Using two genetically marked viral genomes with two VPg sequences, we now provide evidence that this deletion occurs via homologou… Show more

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Cited by 25 publications
(30 citation statements)
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“…This suggested the possibility that a free N terminus is required for VPg's function in uridylylation, and we designed an in vivo experiment to test this hypothesis. We have previously made the interesting observation that a genetically engineered PV containing two VPgs in tandem was quasi-infectious and, upon replication, it rapidly deleted the C-terminal VPg via homologous recombination (13). This mechanism was eliminated in a variant in which the nucleotide sequence of one of the VPgs was engineered such that it differed 36% from that of the wt ( Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…This suggested the possibility that a free N terminus is required for VPg's function in uridylylation, and we designed an in vivo experiment to test this hypothesis. We have previously made the interesting observation that a genetically engineered PV containing two VPgs in tandem was quasi-infectious and, upon replication, it rapidly deleted the C-terminal VPg via homologous recombination (13). This mechanism was eliminated in a variant in which the nucleotide sequence of one of the VPgs was engineered such that it differed 36% from that of the wt ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…(i) Construction of rV3-3B1(Y3F). We have previously constructed a PV cDNA clone that contained two 3B regions in tandem (13). Both 3Bs contained a K/R change at position 10 of the VPg sequence ( Fig.…”
Section: Methodsmentioning
confidence: 99%
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