Genetic variation of the VP1 gene of the virulent duck hepatitis A virus type 1 (DHAV-1) isolates in Shandong province of China

Gao, Jiming; Chen, Junhao; XingKui, Si; Xie, Zhixun; Zhu, Yanli; Zhang, Xingxiao; Wang, Shujing; Jiang, Shijin

doi:10.1007/s12250-012-3255-8

Cited by 35 publications

(36 citation statements)

References 20 publications

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“…However, in another publication, Li et al [22] suggested that DHAV-1 was prevalent in China. Although there was no consistency in the study of prevalence for DHAV genotypes DHAV-1 or DHAV-3 in China, and likewise in Vietnam, the co-existence of both genotypes has been clearly reported and confirmed [4,8,19,20,22].…”

Section: Discussionmentioning

confidence: 89%

“…However, little was known about its molecular characteristics. Recently, the complete genome sequences of many DHV strains were determined in China, Korea, and Taiwan [4][5][6][7][8][9], which enable us to better understand the genomic organization and precise taxonomic position of DHV, indicating that it is genetically and functionally distinct from enteroviruses. Accordingly, DHV is classified as a member of a novel genus, Avihepatovirus, within the family Picornaviridae, and was changed to duck hepatitis A virus (DHAV), classified into three genotypes: DHAV-1, 2, and 3 (see ICTV…”

Section: Discussionmentioning

confidence: 99%

“…There is no cross-neutralization between genotypes [5,6]. According to previous studies, DHAV-1 is widely distributed in China [7][8][9], DHAV-3 has been reported in Korea and China [5], whereas DHAV-2 has been detected only in Taiwan [6]. Limited data are available regarding DHAV viruses in other countries, including Vietnam, although severe outbreaks of DHAV have occurred in ducklings annually.…”

Section: Introductionmentioning

confidence: 99%

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Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam

Doan

Thi

et al. 2016

J Infect Dev Ctries

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Introduction: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during [2009][2010][2011][2012][2013]. Methodology: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified by polymerase chain reaction (PCR). Then, VP1 genes of eleven positive samples and two attenuated vaccine strains were sequenced and analyzed. Results: Genotypic and phylogenetic analyses indicated that the 13 Vietnamese isolates were classified into two genotypes, DHAV-1 and DHAV-3. The rate of identity and homology was 91%-100% between the 10 Vietnamese and 26 global strains of DHAV-3, and 92%-100% between 3 Vietnamese and 16 strains of DHAV-1. Between the DHAV-3 and DHAV-1 strains, the divergence reached 30%. At the C-terminal of VP1 for the different strains, a hypervariable region was observed, and notably, six of the Vietnamese DHAV-3 strains in this study showed four consistent differences (at positions T184M, Q200H, K207N, and K214R) within this group that were distinct from all other DHAV-3 strains. Conclusions: This is the first report of molecular characterization of DHAVs in Vietnam. At least two genotypes were identified, DHAV-1 and DHAV-3, with diversified clades within and between genotypes. DHAV-3 seemed to be dominant in Vietnam.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam

Doan

Thi

et al. 2016

J Infect Dev Ctries

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“…To date, several DHAV sequences from Korea, China, and Taiwan have been published [7,11,12,18,19] . In this study, we reported for the first time in an international journal the complete genome sequences and analysis of three DHAV-3 strains isolated from outbreaks in Vietnam, including DN2 (isolated in Dong Nai, 2009), NC (isolated in Ho Chi Minh DOAN, LE, DO NGUYEN, LE [17] with bootstrap values of 1000 replicas (shown at each branch).…”

Section: Discussionmentioning

confidence: 99%

“…DHAV has three genotypes, i.e., DHAV-1, DHAV-2, and DHAV-3 [6][7][8][9] , are of the most virulent variants [2,10] . Currently, DHAV-1 distributes globally, whereas DHAV-3 has been reported only in China and Korea [6,7,11,12] and DHAV-2 is limited to Taiwan [8] . Recently, the in vivo distribution of both DHAV-1 and DHAV-3 viruses in clinically infected ducklings was reported in Chinese flocks [13,14] .…”

Section: Introductionmentioning

confidence: 99%

Sekans ve Filogenetik Analiz Vietnamda Ördek Hepatitis A Virüs Genotip 3’ün Prevalansını Ortaya Koymaktadır

Doan¹,

Le²,

Thi³

et al. 2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleDoan HT, Le XK, Do R, Nguyen K, Le TH: Sequencing and phylogenetic analysis reveal the prevalence of duck hepatitis A virus genotype 3 in Vietnam. Kafkas Univ Vet Fak Derg, 23 (3): 369-376, 2017. DOI: 10.9775/kvfd.2016 Abstract Duck hepatitis A virus (DHAV) causes an acute and highly contagious disease in young ducklings worldwide. Despite the widespread use of the DHAV vaccine, many outbreaks still occur in Vietnam. In this study, we determined the full-length genome sequence of two DHAV isolates (NC and NT) obtained from infected ducks in 2009 and 2013, and compared them with the attenuated DHAV-1 vaccine strain (namely, VXXT) currently used in Vietnam. The NC and NT strains belong to the virulent DHAV-3 genotype, and their genomes consist of 7791 and 7790 nucleotides (nt), respectively. Both genomes contain one large open reading frame (ORF) of 6756 nt, encoding a polyprotein of 2251 amino acids and possess a typical picornavirus genome organization. The majority of predicted C-terminal cleavage sites in the polyprotein were Q/S or Q/G. Phylogenetic analysis of the nucleotide sequence of the full ORF revealed that all virulent Vietnamese strains are closely related to Chinese DHAV-3 strains. Noticeably, the virulent Vietnamese DHAV-3 strains had relatively low nucleotide identity with the DHAV-1 vaccine strain (73.5%). The study showed that an antigenicity-matched DHAV-3 vaccine is urgently required for use in Vietnam. Keywords: Duck hepatitis A virus, Genotype, Genome, Phylogenetic Sekans ve Filogenetik Analiz Vietnamda Ördek Hepatitis A Virüs Genotip-3'ün Prevalansını Ortaya Koymaktadır Özet

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Circulation and in vivo distribution of duck hepatitis A virus types 1 and 3 in infected ducklings

et al. 2015

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The circulation of duck hepatitis A virus types 1 (DHAV-1) and 3 (DHAV-3) in Southeast Asia has resulted in a continuously changing epidemiological scenario. In this study, a duplex real-time PCR assay for simultaneous quantitative detection of DHAV-1 and DHAV-3 was established, and 200 liver samples from dead ducklings collected from 31 different flocks in Shandong province, China, were tested. Fifty-eight (29.0 %) samples from 13 flocks were positive for DHAV-1 single infection, 113 (56.5 %) samples from 13 other flocks were positive for DHAV-3 single infection, and 24 samples (12.0 %) from four flocks were positive for both viruses. DHAV-1 and DHAV-3 were detected with high viral loads in all of the organs tested (liver, spleen, pancreas, kidney, heart, thymus, bursa of Fabricius and brain). No significant difference in DHAV-1 and DHAV-3 viral loads was found between singly infected and coinfected samples, and there was no correlation between the viral loads of the two viruses and the age of dead ducklings. To the best of our knowledge, this is the first report about the in vivo distribution of DHAV-1 and DHAV-3 in clinically infected ducklings.

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Genetic variation of the VP1 gene of the virulent duck hepatitis A virus type 1 (DHAV-1) isolates in Shandong province of China

Cited by 35 publications

References 20 publications

Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam

Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam

Sekans ve Filogenetik Analiz Vietnamda Ördek Hepatitis A Virüs Genotip 3’ün Prevalansını Ortaya Koymaktadır

Circulation and in vivo distribution of duck hepatitis A virus types 1 and 3 in infected ducklings

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