Genetic variations and sequences analysis of MTATP6 and MTATP8 genes among different Chinese pig breeds

Abstract

To investigate the genetic variations of mtATP6 and mtATP8 genes among different Chinese pig breeds, two fragments of 425 and 743 bp containing the whole coding region of mtATP8 and mtATP6 genes were amplified with 805 individuals from 23 Chinese local pig breeds, three types of Chinese wild boars and three European pig breeds. Sequence comparison identified a total number of 17 substitutions including six variable sites in mtATP8 and eleven substitutions in mtATP6 gene. The restriction enzyme Fok I revealed four polymorphic sites (nt8086, 8176, 8514 and 7784), and four RFLP haplotype patterns (A, B, C and D) were identified in mtATP6 and mtATP8 genes among all tested samples. Our data showed AC combined haplotype originated from Asia and BD was regarded as European origin. The average frequency of Asian mtDNA haplotypes was 38.3% across the investigated European breeds but varied within each breeds (13.3∼76.7%). Phylogenetic analyses were performed also considering some published sequences in the databases; the sequences were divided into three distinct groups, denoted A, E1, and E2. The Asian AC haplotype existed among the European domestic pigs was fully consistent with the results of previous molecular studies and well-documented history. This study will help us to better understand the genetic variations of mitochondrial genes among different Chinese pig breeds.

Mitochondrial DNA Sequence and Phylogenetic Evaluation of Geographically DisparateSus scrofaBreeds

“…Observations such as these suggest that it would not be necessary for polymorphisms in proteins of the electron transport chain to cause dramatic changes in protein function, as subtle differences in proton motive force could meaningfully impact feed efficiency. Despite low bootstrap values for the fine structure of the tree, our data illustrate a phylogenic relationship that is consistent with features of trees reported previously and show that Mangalica swine are most closely related to European breeds (22,23,26,27). The grouping of Turopolje and Mangalica breeds with European swine is unsurprising.…”

“…It was noted that haplotypes observed within a single herd indeed vary (32). Previously published swine phylogenies often support the relationship of Yorkshire breeds with other European breeds, and it may be important to consider nuclear loci in any phylogeny of swine in addition to mtDNA sequences in order to obtain an accurate evolutionary lineage (22)(23)(24)(25)(26)(27)(28). The observation that both Asian and European haplotypes still exist may suggest that none of the haplotypes studied were associated with more optimal production or metabolic characteristics.…”