miR-222 is involved in the regulation of genistein on skeletal muscle fiber type

Gan, Mailin; Shen, Linyuan; Liu, Lin; Guo, Zhixian; Wang, Shujie; Chen, Lei; Zheng, Ting; Yuan, Fan; Tan, Yimin; Jiang, Dongmei; Li, Xuewei; Zhang, Shunhua; Zhu, Li

doi:10.1016/j.jnutbio.2019.108320

Cited by 12 publications

(6 citation statements)

References 37 publications

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“…GEN has been reported to affect skeletal muscle development by regulating protein degradation [ 30 ], immunity [ 31 ], oxidative stress [ 32 ], and myotube differentiation [ 22 ]. Notably, GEN has been reported to regulate the expression of miR-1260b [ 19 ], miR-223 [ 20 ], miR-155 [ 33 ], miR-20a [ 34 ], miR-451 [ 35 ], miR-221/222 [ 36 ], etc.…”

Section: Discussionmentioning

confidence: 99%

“…Notably, GEN has been reported to regulate the expression of miR-1260b [ 19 ], miR-223 [ 20 ], miR-155 [ 33 ], miR-20a [ 34 ], miR-451 [ 35 ], miR-221/222 [ 36 ], etc. Among them, miR-222 expression was inhibited by GEN in PC-3 cells [ 36 ], MCF-7 cells [ 37 ], and U87-MG cells [ 38 ], adipocytes [ 21 ], myoblasts [ 22 ]. Intriguingly, this regularity is not found in other miRNAs.…”

Section: Discussionmentioning

confidence: 99%

“…Genistein (GEN) is a phytoestrogen that exerts estrogenic or antiestrogenic effects in mammals [ 18 ]. In previous studies, it was reported that GEN could play a role in inhibiting cancer cells [ 19 , 20 ], improving obesity [ 21 ], and modulating skeletal muscle development [ 22 ] by regulating the expression of miRNAs. In this study, we report that GEN can act as a natural regulator of miR-221 and miR-222.…”

Section: Introductionmentioning

confidence: 99%

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Genistein Promotes Skeletal Muscle Regeneration by Regulating miR-221/222

Shen

Liao

Chen

et al. 2022

IJMS

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Genistein (GEN), a phytoestrogen, has been reported to regulate skeletal muscle endocrine factor expression and muscle fiber type switching, but its role in skeletal muscle regeneration is poorly understood. As a class of epigenetic regulators widely involved in skeletal muscle development, microRNAs (miRNAs) have the potential to treat skeletal muscle injury. In this study, we identified miR-221 and miR-222 and their target genes MyoG and Tnnc1 as key regulators during skeletal muscle regeneration, and both were regulated by GEN. C2C12 myoblasts and C2C12 myotubes were then used to simulate the proliferation and differentiation of muscle satellite cells during skeletal muscle regeneration. The results showed that GEN could inhibit the proliferation of satellite cells and promote the differentiation of satellite cells by inhibiting the expression of miR-221/222. Subsequent in vitro and in vivo experiments showed that GEN improved skeletal muscle regeneration mainly by promoting satellite cell differentiation in the middle and late stages, by regulating miR-221/222 expression. These results suggest that miR-221/222 and their natural regulator GEN have potential applications in skeletal muscle regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genistein Promotes Skeletal Muscle Regeneration by Regulating miR-221/222

Shen

Liao

Chen

et al. 2022

IJMS

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“…Skeletal muscle is well known as one of the fundamental tissues in regulating energy metabolism ( 29 , 30 ). Obesity-related high-fat condition consistently causes metabolic disturbances in the skeletal muscle, which, in turn, aggravates systemic metabolic syndrome, especially when aging ( 31 – 33 ).…”

Section: Discussionmentioning

confidence: 99%

Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism

Mei

Chen

Zhang

et al. 2022

Food & Nutrition Research

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Background: Globally, obesity is a significant public problem, especially when aging. Sesamol, a phenolic lignan present in sesame seeds, might have a positive effect on high-fat diet (HFD)-induced obesity associated with aging. Objective: The purpose of current research study was to explore salutary effects and mechanisms of sesamol in treating alimentary obesity and associated metabolic syndrome in middle-aged mice. Methods: C57BL/6J mice aged 4–6 weeks and 6–8 months were assigned to the young normal diet group, middle-aged normal diet group, middle-aged HFD group, and middle-aged HFD + sesamol group. At the end of experiment, glucose tolerance test and insulin tolerance test were performed; the levels of lipids and oxidative stress-related factors in the serum and skeletal muscle were detected using chemistry reagent kits; lipid accumulation in skeletal muscle was observed by oil red O staining; the expressions of muscular glucose and lipid metabolism associated proteins were measured by Western blotting. Results: Sesamol decreased the body weight and alleviated obesity-associated metabolism syndrome in middle-aged mice, such as glucose intolerance, insulin resistance, dyslipidemia, and oxidative stress. Moreover, muscular metabolic disorders were attenuated after treatment with sesamol. It increased the expression of glucose transporter type-4 and down-regulated the protein levels of pyruvate dehydrogenase kinase isozyme 4, implying the increase of glucose uptake and oxidation. Meanwhile, sesamol decreased the expression of sterol regulatory element binding protein 1c and up-regulated the phosphorylation of hormone-sensitive lipase and the level of carnitine palmityl transferase 1α, which led to the declined lipogenesis and the increased lipolysis and lipid oxidation. In addition, the SIRT1/AMPK signaling pathway was triggered by sesamol, from which it is understood how sesamol enhances glucose and lipid metabolism. Conclusions: Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism, which might be associated with the stimulation of the SIRT1/AMPK pathway.

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“…Skeletal muscle exhibits contractile and stretchable characteristics 1 . Muscle fiber can be divided into type Ι (slow‐twitch, slow oxidation), type IIa (fast‐twitch, fast oxidative), type IIb (fast‐twitch, fast glycolytic), and type IIx (fast‐twitch, intermediate), and type Ι and type II can transform into each other 2, 3 . Types Ι and IIa primarily show oxidation metabolism, whereas types IIb and IIx mainly exhibit glycolytic metabolism.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary ferulic acid supplementation on growth performance and skeletal muscle fiber type conversion in weaned piglets

et al. 2021

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BACKGROUND Ferulic acid (FA) is a common polyphenolic compound. The purpose of this study was to explore the effect of dietary FA supplementation on growth performance and muscle fiber type conversion in weaned piglets. In this study, eighteen 21‐day‐old DLY (Duroc × Landrace × Yorkshire) weaned piglets were randomly divided into control, 0.05% FA, and 0.45% FA groups. RESULTS Our study showed that dietary FA supplementation had no effect on growth performance, but it could upregulate the expression of slow myosin heavy chain (MyHC) protein, increase the activities of succinic dehydrogenase and malate dehydrogenase, and downregulate the expression of fast MyHC protein. Dietary FA supplementation also increased the expression levels of phosphorylated AMP‐activated protein kinase, sirtuin 1 (Sirt1), peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha (PGC‐1α), myocyte enhancer factor 2C, and troponin I‐SS, increased the proportion of slow‐twitch fiber, and decreased the proportion of fast‐twitch fiber. In addition, our results showed that dietary FA supplementation increased the messenger RNA abundance of mitochondrial nuclear transcription genes, including ATP synthase membrane subunit c locus 1, cytochrome oxidase subunit 1, nuclear respiratory factor 1, mitochondrial transcription factor A, mitochondrial transcription factor B1, and cytochrome c. CONCLUSION We provided the first evidence that FA could promote muscle fiber type conversion from fast‐twitch to slow‐twitch via the Sirt1/AMP‐activated protein kinase/PGC‐1α signaling pathway and could improve the mitochondrial function in weaned piglets. This means that FA can be used as a dietary supplement to improve the quality of pork. © 2021 Society of Chemical Industry

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miR-222 is involved in the regulation of genistein on skeletal muscle fiber type

Cited by 12 publications

References 37 publications

Genistein Promotes Skeletal Muscle Regeneration by Regulating miR-221/222

Genistein Promotes Skeletal Muscle Regeneration by Regulating miR-221/222

Sesamol counteracts on metabolic disorders of middle-aged alimentary obese mice through regulating skeletal muscle glucose and lipid metabolism

Effects of dietary ferulic acid supplementation on growth performance and skeletal muscle fiber type conversion in weaned piglets

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