Genetic variations of hepatitis B virus and serum aflatoxin-lysine adduct on high risk of hepatocellular carcinoma in Southern Guangxi, China

Abstract

Background & Aims Southern Guangxi area is one of the endemic areas for hepatocellular carcinoma (HCC) in China. This study evaluates the roles of genetic variations of hepatitis B virus (HBV) and aflatoxin B1 (AFB1) exposure in the formation of HCC in this high-risk area. Methods The study recruited 60 HCC patients and 120 age-, gender-, residency-matched controls. HBV genotype and basic core promoter (BCP) mutations were determined by nested-PCR/direct sequencing. Serum AFB1-lysine adduct was measured by high performance liquid chromatography-fluorescence detection. Results HBV Genotype C was predominant in 75.0% of cases and 84.2% of controls. The 1762T/1764A double mutations, 1753V mutations, and 1752V mutations were associated with HCC risk evidenced by the adjusted odds ratio (OR) [95% confidence interval (95% CI)] of 3.89 (1.40–10.77), 2.87 (1.49–5.49), and 5.96 (1.75–20.25), respectively. The adjusted OR (95% CI) was 6.94 (1.68–27.78) for subjects with 1762T/1764A double mutations and high AFB1-lysine adduct level; 2.01 (0.24–14.29), for those with only 1762T/1764A double mutations; and 4.26 (1.16–15.38) for those with only high AFB1-lysine adduct level, respectively. The adjusted OR was 5.13 (1.79–14.71) for subjects with 1753V mutations and high AFB1-lysine adduct level; 1.20 (0.47–3.08) for those with only 1753V mutations, and 2.28 (1.01–5.31) for those with high AFB1-lysine adduct level, respectively. Conclusions These data confirmed the association of BCP mutations with HCC risk and the additive effects of 1762T/1764A double mutations and 1753V mutations with dietary AFB1 exposure in this high-risk area for HCC.

Hepatitis B Virus Gene Mutations and Hepatocarcinogenesis

“…were also associated with the progression of liver disease, such as HCC (Tanaka et al, 2006;Malik et al, 2012). Additionally, Li et al (Xu et al, 2010). evaluated the roles of genetic variations of HBV in the development of HCC supported the hypothesis that both the A1762T/G1764A double mutations and the 1753V/1752V mutations were associated with increased risk for HCC.…”

Evaluation of KIF23 variant 1 expression and relevance as a novel prognostic factor in patients with hepatocellular carcinoma

“…Hepatocellular carcinoma (HCC), the major type of primary liver cancer, is one of the most prevalent cancers in the world [ 1 ]. China is one of the high-risk areas for HCC due to the high prevalence of chronic hepatitis B virus infection [ 2 , 3 ], representing more than half of the cases in the entire world [ 4 ]. Despite the remarkable advances in diagnostic and therapeutic techniques, prognosis of HCC still remains extremely poor, ranking as the third leading cause of cancer-related death worldwide [ 1 ].…”

Protein Phosphatase Magnesium-Dependent 1δ (PPM1D) mRNA Expression Is a Prognosis Marker for Hepatocellular Carcinoma

“…Hepatocellular carcinoma (HCC) is the fifth most common cancer , and the third leading cause of cancer-related death worldwide[1]. Surgical resection of the tumor offers a chance for cure, but does not achieve optimal long-term survival, mainly due to recurrence and metastasis[2].…”