Genetically variable triplet repeats in a RING-finger ORF of Helicoverpa species baculoviruses

A Chrysodeixis chalcites Single-Nucleocapsid Nucleopolyhedrovirus Population from the Canary Islands Is Genotypically Structured To Maximize Survival

“…To design these primers, five genomic regions of ChchTF1 were sequenced. These regions were highly variable, according to the ChchSNPV-NL sequence information (GenBank accession number AY864330; 29) and other reports (37,38). These regions were outside the complete coding regions of hoar (chch4; nucleotides [nt] 2,965 to 6,504 in the ChchSNPV-NL genome), bro-a (chch55; nt 48,823 to 50,376), bro-b (chc69; nt 68,861 to 66954), bro-c (chch70; nt 70,060 to 69,254), and bro-d (chc114; nt 114,806 to 113,517) ( Table 1).…”

“…Phenotypic differences are derived from changes at the genome level (40,41) and are important for virus adaptation and survival (8,42). The major source of intraspecific variability among genotypes can be found in homologous-repeat regions (hrs) (5), the bro gene family (5,11,37), and the hoar gene (38). For ChchTF1 genotypes, the greatest variability was observed within the hoar and bro-d genes.…”

Identification of Spodoptera exigua nucleopolyhedrovirus genes involved in pathogenicity and virulence

“…* Figures in bold indicate significance, that is, a relative potency significantly lower or higher than that of SeBacAL1. 1999), has an unusual codon bias and displays genetic variability suggesting frequent mutations in this locus (Le et al, 1997). It has been suggested that hoar may interfere with host defenses and the high mutation rate could be an adaptation that favors infection across different host species (Le et al, 1997).…”

“…1999), has an unusual codon bias and displays genetic variability suggesting frequent mutations in this locus (Le et al, 1997). It has been suggested that hoar may interfere with host defenses and the high mutation rate could be an adaptation that favors infection across different host species (Le et al, 1997). Additionally HOAR has been found in the ODV membrane in Helicoverpa armigera NPV (Hou et al, 2013), and in midgut cells of Mamestra configurata larvae during infection by M. configurata NPV-A (Donly et al, 2014).…”

Complete Genome Sequences of Five Chrysodeixis chalcites Nucleopolyhedrovirus Genotypes from a Canary Islands Isolate

“…A whole-genome sequence alignment between ChchTF1-A, ChchTF1-B, ChchTF1-C, ChchTF1-G, and ChchTF1-H and ChchSNPV-NL shows 98 to 99% homology at the nucleotide level. This analysis also demonstrated that variable genomic regions are located principally in the hoar and bro-d genes, which represent a major source of intraspecific variability among genotypes in many baculoviruses (7–9). Finally, phylogenetic analysis grouped the five Spanish genotypes and the Dutch genotype in three pairs of clusters: ChchSNPV-NL with ChchTF1-G, ChchTF1-A with ChchTF1-B, and ChchTF1-C with ChchTF1-H.…”