Genetics and Biochemistry of Biphenyl and PCB Biodegradation

Agulló, Loreine; Pieper, Dietmar H.; Seeger, Michael

doi:10.1007/978-3-319-50418-6_30

Cited by 10 publications

(4 citation statements)

References 133 publications

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“…Considering that PCBs pollution is typically caused by mixtures of congeners [39], we evaluated the [12,16,18,40,41] or strains isolated from PCBs-J o u r n a l P r e -p r o o f polluted soils [29,42]. Nevertheless, fungal PCBs removal is generally affected by initial PCBs concentration [42] and to our best knowledge only Ruiz Aguilar et al [41] assayed PCBs removal by white-rot fungi in liquid culture at higher concentrations.…”

Section: Discussionmentioning

confidence: 99%

Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

Sadañoski

Benítez

Fonseca

et al. 2019

Journal of Environmental Chemical Engineering

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Section: Discussionmentioning

confidence: 99%

Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

Sadañoski

Benítez

Fonseca

et al. 2019

Journal of Environmental Chemical Engineering

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“…Polychlorinated biphenyls (PCBs) are a group of chlorinated organic pollutants that have been used in a wide range of products in the 20th century (Agulló et al . 2019). Although their production and usage were banned in the late 1970s, increasing attention has been paid to the elimination of PCBs because of their high persistence, bioaccumulation and toxicity linked to diseases of the immune, reproductive, nervous and endocrine systems (Fan et al .…”

Section: Introductionmentioning

confidence: 99%

Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment

et al. 2021

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Aims To evaluate the mycoremediation of polychlorinated biphenyls (PCBs) by either single cultures or binary consortia of Pleurotus pulmonarius LBM 105 and Trametes sanguinea LBM 023. Methods and Results PCBs tolerance, removal capacity, toxicity reduction and ligninolytic enzyme expression were assessed when growing single culture and binary consortium of fungus in 217 mg l−1 of a technical mixture of Aroclor 1242, 1254 and 1260 in transformer oil. A decrease in tolerance and variation in ligninolytic enzyme secretion were observed in PCB‐amended solid media. Pleurotus pulmonarius LBM 105 mono‐culture was able to remove up to 95·4% of PCBs, whereas binary consortium and T. sanguinea LBM 023 could biodegrade about 55% after 24 days. Significant detoxification levels were detected in all treatments by biosorption mechanism. Conclusions Pleurotus pulmonarius LBM 105 in single culture had the best performance regarding PCBs biodegradation and toxicity reduction. Ligninolytic enzyme secretion changed in co‐culture. Significance and Impact of the Study The evaluation of PCBs bioremediation effectiveness of basidiomycetes consortium in terms of PCB removal, toxicity and ligninolytic enzyme production to unravel the differences between using individual cultures or consortium has not been reported. The results from this study enable the selection of P. pulmonarius LBM 105 mono‐culture to bioremediate PCBs as it showed higher efficiency compared to binary consortium with T. sanguinea LBM 023 for potential decontamination of PCB‐contaminated transformer oil.

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“…This presence of chlorobenzoates as dead‐end metabolites in PCB biodegradation presents a problem as they are toxic to the PCB degraders . Typically, PCBs consist of at least 60 different congeners, and microorganisms were shown to act on only a fraction of these . This prompted research into various ways to optimize the activities of related enzymes in PCB biodegradation .…”

mentioning

confidence: 99%

“…This prompted research into various ways to optimize the activities of related enzymes in PCB biodegradation . However, most of the research on enzyme optimization was mainly directed toward biphenyl‐1,2‐dioxygenase, as this is the key enzyme that specifies the types of congeners to be degraded by a PCB degrader . The presence of dead‐end metabolites presents another problem as they halt the biodegradation process, and hence the need for research on how to deal with them .…”

mentioning

confidence: 99%

Dechlorination of polychlorobiphenyl degradation metabolites by a recombinant glutathione S‐transferase from Acidovorax sp. KKS102

Shehu

Alias

2019

FEBS Open Bio

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A glutathione S ‐transferase ( GST ) with a potential dehalogenation function against various organochlorine substrates was identified from a polychlorobiphenyl ( PCB )‐degrading organism, Acidovorax sp. KKS 102. A homolog of the gene BphK (biphenyl upper pathway K), named BphK‐ KKS , was cloned, purified and biochemically characterized. Bioinformatic analysis indicated several conserved amino acids that participated in the catalytic activity of the enzyme, and site‐directed mutagenesis of these conserved amino acids revealed their importance in the enzyme's catalytic activity. The wild‐type and mutant (C10F, K107T and A180P) recombinant proteins displayed wider substrate specificity. The wild‐type recombinant GST reacted towards 1‐chloro‐2,4‐dinitrobenzene ( CDNB ), ethacrynic acid, hydrogen peroxide and cumene hydroperoxide. The mutated recombinant proteins, however, showed significant variation in specific activities towards the substrates. A combination of a molecular docking study and a chloride ion detection assay showed potential interaction with and a dechlorination function against 2‐, 3‐ and 4‐chlorobenzoates (metabolites generated during PCB biodegradation) in addition to some organochlorine pesticides (dichlorodiphenyltrichloroethane, endosulfan and permethrin). It was demonstrated that the behavior of the dechlorinating activities varied among the wild‐type and mutant recombinant proteins. Kinetic studies (using CDNB and glutathione) showed that the kinetic parameters K m , V max , K cat and K m / K cat were all affected by the mutations. While C10F and A180P mutants displayed an increase in GST activity and the dechlorination function of the enzyme, the K107T mutant displayed variable results, suggesting a functional role of Lys107 in determining substrate specificity of the enzyme. These results demonstrated that the enzyme should be valuable in the bioremediation of metabolites generated during PCB biodegradation.

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Genetics and Biochemistry of Biphenyl and PCB Biodegradation

Cited by 10 publications

References 133 publications

Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment

Dechlorination of polychlorobiphenyl degradation metabolites by a recombinant glutathione S‐transferase from Acidovorax sp. KKS102

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