Genetics of Asthma

Walley, Andrew J.; Cookson, William

doi:10.1007/978-3-0348-8784-7_2

Cited by 2 publications

(1 citation statement)

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“…Genetic defects in cytokines, such as interleukin 4 (IL-4) cluster, IL-12 receptor, IL-13 promoter, IL-4 receptor (IL-4R), stem cell factor (SCF), and tumor necrosis factor (TNF)-α, have been linked to the development of eczema; with cytokines play a crucial role in immune responses. [6][7][8][9][10][11] The cause-and-effect relationship between a specific locus and the occurrence of the AD phenotype has not been confirmed despite reports describing an association between the genetic locus and an atopy symptom. [12] The expression of IL-24 is elevated in inflammatory skin diseases such as AD or psoriasis.…”

Section: Eczema (Atopic Dermatitis [Ad]mentioning

confidence: 99%

Serum level of interleukin-24 and its polymorphism in eczematic Iraqi patients

Mahmood,

Al-Bassam

2024

Medicine

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Eczema is a common skin disease associated with inflammation. Interleukin (IL)-24 is crucial in the pathogenesis of inflammatory diseases like eczema. The study objective was the assessment of IL-24 serum levels and its gene polymorphisms in eczematic Iraqi patients. This retrospective case-control study involved 145 participants, divided into 82 patients with eczema and 63 healthy controls. An enzyme-linked immunosorbent assay measured serum IL-24, while polymerase chain reaction and Sanger DNA sequencing were used for genotype analysis. Serum IL-24 level was significantly higher (P value < .001) in patients compared to controls (41.6 [interquartile range (IQR): 28.9–53.6] vs 9.8 [IQR: 0.8–19.6] pg/mL, respectively). DNA sequence illustrated 2 SNPs with polymorphic frequencies (rs1150256 G/A and rs3093425 del/ins). The first SNP (rs1150256 G/A) showed 3 genotypes (GG, AA, and G/A), while the second SNP (rs3093425) showed 3 genotypes (-/G del/Ins, G Ins/Ins, and - del/del). The subsequent investigation revealed the presence of the following findings within the DNA sequence of the PCR amplified region (329bp). In the control group, all participants had GG/G (wild type) genotype/allele for the rs1150256 SNP, while in eczematic patients, 24.4% GG, 50% GA, and 25.6% AA. For the second SNP genotype (rs3093425 del/ins), the genotype frequencies in patients vs control were (24.4% vs 84.1%, 50.0% vs 11.1%, and 25.6% vs 4.8; Del/Del, Del/Ins, and Ins/Ins, respectively). The presence of Ins compared to Del increased the risk of eczema by 8.91 (4.66–17.03); OR (95% CI). In conclusion, IL-24 is a good predictor of eczema and A-allele carrier for rs1150256 SNP, and insertion-allele carrier for rs3093425 SNP is associated with elevated serum IL-24 and higher risk of eczema.

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