Genetics of the DST-mediated mRNA decay pathway using a transgene-based selection

Lidder, Preetmoninder; Johnson, Mark A.; Sullivan, Michael L.; Thompson, Debrah M.; Pérez‐Amador, Miguel A.; Howard, Chris; Green, P.J.

doi:10.1042/bst0320575

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…AtFER1 mRNA stability was also studied in two trans ‐acting mutants, named dst1 and dst2 , previously identified in a genetic screen aimed at isolating factors involved in the DST‐dependent mRNA degradation pathway (Johnson et al , 2000; Pérez‐Amador et al , 2001; Lidder et al , 2004). AtFER1 mRNA accumulation in response to Fe was measured in Col, dst1 and dst2 grown in liquid medium (Figure 5A).…”

Section: Resultsmentioning

confidence: 99%

“…A genome‐wide study in Arabidopsis showed that the DST sequence was enriched in unstable transcripts (Narsai et al , 2007). Three trans ‐acting components of the DST pathway, named DST1–3, were isolated by a forward genetic approach (Johnson et al , 2000; Pérez‐Amador et al , 2001; Lidder et al , 2004). Molecular and phenotypic analysis of the dst mutants showed deregulation of functional subsets of genes including some circadian clock‐related genes (Pérez‐Amador et al , 2001).…”

Section: Introductionmentioning

confidence: 99%

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Iron and ROS control of the DownSTream mRNA decay pathway is essential for plant fitness

et al. 2011

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A new regulatory pathway involved in plant response to oxidative stress was revealed using the iron-induced Arabidopsis ferritin AtFER1 as a model. Using pharmacological and genetic approaches, the DownSTream (DST) cis-acting element in the 3'-untranslated region of the AtFER1 mRNA was shown to be involved in the degradation of this transcript, and oxidative stress triggers this destabilization. In the two previously identified trans-acting mutants (dst1 and dst2), AtFER1 mRNA stability is indeed impaired. Other iron-regulated genes containing putative DST sequences also displayed altered expression. Further physiological characterization identified this oxidative stress-induced DST-dependent degradation pathway as an essential regulatory mechanism to modulate mRNA accumulation patterns. Alteration of this control dramatically impacts plant oxidative physiology and growth. In conclusion, the DST-dependent mRNA stability control appears to be an essential mechanism that allows plants to cope with adverse environmental conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Iron and ROS control of the DownSTream mRNA decay pathway is essential for plant fitness

et al. 2011

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“…For several circadian genes, a specific post-transcriptional mRNA decay mechanism has been postulated [34]. The circadian control of messenger stability was found to be associated to a sequence specific decay pathway [34,35]. Instability determinants of clock controlled mRNAs might be located within untranslated regions, introns or within the coding region as shown for mRNA of CIR-CADIAN CLOCK ASSOCIATED 1 (CCA1) [36].…”

Section: Transcriptional Control Of Sut1 Post-transcriptional Contromentioning

confidence: 99%

Phloem Mobility and Stability of Sucrose Transporter Transcripts

He¹,

Chincinska²,

Hackel³

et al. 2008

TOPSJ

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Sucrose transporters are essential membrane proteins for the distribution of photoassimilates in higher plants. In Solanaceous species the proteins of all known sucrose transporters are co-localized in enucleate sieve elements and undergo permanent turnover. The mRNA of the sucrose transporter StSUT1 is localized in both, sieve elements and companion cells. Sucrose transporter mRNAs have been detected in the phloem sap of several species. Here, we analyzed the mobility of sucrose transporter transcripts in grafted plants and between host and parasitic plants. Phloem-mobility was found when a c-myc tagged SUT1-fusion construct without untranslated regions (UTRs) was expressed under the CaMV 35S promoter. We conclude that neither 3'-nor 5'-UTRs are essential for mRNA transport through plasmodesmata. Cycloheximide, which inhibits translation, has also effects on SUT transcript stability. Whereas SUT1 transcripts are destabilized when translation is inhibited, SUT2 and SUT4 transcripts accumulate up to 4fold under these conditions. Inhibitor studies revealed post-transcriptional regulation of SUT2 and SUT4 transcript accumulation. A model is proposed explaining the coordination of SUT expression in Solanaceae.

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Specific cleavage of ribosomal RNA and mRNA during victorin‐induced apoptotic cell death in oat

Hoat¹,

Nakayashiki²,

Tosa³

et al. 2006

The Plant Journal

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SummaryHere we report that rRNA and mRNA are specifically degraded in oat (Avena sativa L.) cells during apoptotic cell death induced by victorin, a host-selective toxin produced by Cochliobolus victoriae. Northern analysis indicated that rRNA species from the cytosol, mitochondria and chloroplasts were all degraded via specific degradation intermediates during victorin-induced apoptotic cell death but, in contrast, they were randomly digested in necrotic cell death induced by 30 mM CuSO 4 and heat shock. This indicates that specific rRNA cleavage could be controlled by an intrinsic program. We also observed specific cleavage of mRNA of housekeeping genes such as actin and ubiquitin during victorin-induced cell death. Interestingly, no victorininduced mRNA degradation was detected with stress-responding genes such as PR-1, PR-10 and GPx throughout the experimental period. The RNA degradation mostly, but not always, occurred in parallel with DNA laddering, but pharmacological studies indicated that these processes are regulated by different signaling pathways with some overlapping upstream signals.

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Genetics of the DST-mediated mRNA decay pathway using a transgene-based selection

Cited by 4 publications

References 12 publications

Iron and ROS control of the DownSTream mRNA decay pathway is essential for plant fitness

Iron and ROS control of the DownSTream mRNA decay pathway is essential for plant fitness

Phloem Mobility and Stability of Sucrose Transporter Transcripts

Specific cleavage of ribosomal RNA and mRNA during victorin‐induced apoptotic cell death in oat

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