GeneXpert detection of mycobacterium tuberculosis from sputum collected and transported in a molecular transport medium

Abstract

2 weeks. At the initiation of therapy in each experiment a group of mice (early controls) were euthanized by CO 2 inhalation and their lungs were collected. At the completion of therapy an untreated group of mice, late controls (LC), and treated mice were euthanized. Mycobacterial loads in right lungs were measured by serial dilution and plating on Middlebrook 7H10 agar plates.Results: The mycobacterial loads (log CFU) for the EC, LC, INH oral and INH IN were 3.34, 4.49, 2.94, and 2.82 respectively (exp. 1). The mycobacterial loads for the EC, LC, RZL oral, and RZL IN were 6.26, 8.93, 4.30, and 4.58 respectively (exp. 2). The LC group was euthanized 3 days early due to their advanced illness. IN delivery of INH and RIF was significantly better than the untreated late controls. Conclusion: The activities of INH and RZL by IN delivery in these experiments suggest that other agents that cannot be given orally could be evaluated for their potential therapeutic activities by IN administration.

PrimeStore MTM and OMNIgene Sputum for the Preservation of Sputum for Xpert MTB/RIF Testing in Nigeria

“…Primestore was primarily designed to preserve nucleic acids for molecular testing, rather than preserving cells for culture, and contains reagents to lyse mycobacteria, protecting nucleic acids from nuclease-mediated degradation [7]. Samples preserved in Primestore have been utilised for next generation sequencing [15] to identify drug-resistant mutations.…”

“…One of these reagents, OMNIGene-sputum (Omnigene, DNA Genotek Inc., Canada), is allegedly able to decontaminate and liquefy sputum, whilst preventing sample degradation for up to eight days at ambient temperature [6]. A second reagent, PrimeStore Molecular Transport Medium (Primestore, Longhorn Vaccines & Diagnostics, US) has been reported to inactivate Mycobacterium tuberculosis (MTB) and preserve nucleic acids at ambient temperature, for downstream testing [7]. The inactivation of MTB would improve the biosafety of the laboratory and the transport and handling of samples by staff members.…”