Genic male and female sterility in vegetable crops

Cheng, Zhihua; Song, Weiyuan; Zhang, Xiaolan

doi:10.1093/hr/uhac232

Cited by 14 publications

(7 citation statements)

References 158 publications

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“…Male sterility, which encompasses anther morphological defects, microsporogenesis issues, impaired pollen development, and disrupted pollen function, may also be a contributing factor [ 49 ]. Similarly, female sterility can arise from defects in the ovaries and ovules [ 50 , 51 ]. A previous study exploring the morphology of L. indica identified anther dehiscence and abnormal embryonic sac development as the primary causes of L. indica sterility.…”

Section: Discussionmentioning

confidence: 99%

MADS-Box Family Genes in Lagerstroemia indica and Their Involvement in Flower Development

Qiao,

Deng,

Zeng

et al. 2024

Plants

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MADS-box is a key transcription factor regulating the transition to flowering and flower development. Lagerstroemia indica ‘Xiang Yun’ is a new cultivar of crape myrtle characterized by its non-fruiting nature. To study the molecular mechanism underlying the non-fruiting characteristics of ‘Xiang Yun’, 82 MADS-box genes were identified from the genome of L. indica. The physicochemical properties of these genes were examined using bioinformatics methods, and their expression as well as endogenous hormone levels at various stages of flower development were analyzed. The results showed that LiMADS genes were primarily classified into two types: type I and type II, with the majority being type II that contained an abundance of cis-acting elements in their promoters. By screening nine core proteins by predicted protein interactions and performing qRT-PCR analysis as well as in combination with transcriptome data, we found that the expression levels of most MADS genes involved in flower development were significantly lower in ‘Xiang Yun’ than in the wild type ‘Hong Ye’. Hormonal analysis indicated that ‘Xiang Yun’ had higher levels of iP, IPR, TZR, and zeatin during its early stages of flower development than ‘Hong Ye’, whereas the MeJA content was substantially lower at the late stage of flower development of ‘Hong Ye’. Finally, correlation analysis showed that JA, IAA, SA, and TZR were positively correlated with the expression levels of most type II genes. Based on these analyses, a working model for the non-fruiting ‘Xiang Yun’ was proposed. During the course of flower development, plant hormone response pathways may affect the expression of MADS genes, resulting in their low expression in flower development, which led to the abnormal development of the stamen and embryo sac and ultimately affected the fruiting process of ‘Xiang Yun’.

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Section: Discussionmentioning

confidence: 99%

MADS-Box Family Genes in Lagerstroemia indica and Their Involvement in Flower Development

Qiao,

Deng,

Zeng

et al. 2024

Plants

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“…Cross-pollinating nature and huge inbreeding depression harden inbred lines development in onion, whereas, development of CMS lines through genome editing or RNAi mediated could be possible. Previous, research demonstrated that male sterility was triggered by downregulating some potential genes in various crops, including SAMDC (Cheng et al, 2022), CsSUT1I (Sun et al, 2019), MSH1 (Zhao et al, 2016, AcMSH1 , and CsHT1 (Cheng et al, 2015). In order to develop male sterile lines in Alliums, this gene would either be knocked down or eliminated in onions.…”

Section: Development Of Cms Linementioning

confidence: 99%

Onion: Breeding and Genomics

Mahajan,

Gupta

2023

isvs

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Onion is a long-cultivated vegetable crop that originated in Central Asia. Almost everywhere in the world, onions are cultivated because of their culinary, nutritional, medicinal and health benefits. Using traditional breeding methods, breeders and farmers have been working to enhance onion yield for years. While it is currently difficult to meet growing customer demand and deal with unfavorable climate conditions. Due to its biennial life cycle, cross-pollination and inbreeding depression, it has been challenging to characterize significant traits. Consequently, the recently available onion genome sequence and the use of contemporary molecular breeding techniques will help researchers to overcome those challenges and speed up the development of the onion crop.

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“…However, the traditional method of producing tomato hybrid seeds involves labor-intensive processes, such as manual emasculation and hand pollination, leading to increased costs and the risk of self-pollination impurities [ 3 , 4 ]. To address these challenges, researchers have explored the suitability of male sterile plants as female progenitors in the production of tomato hybrid seeds [ 4 , 5 ]. Different genes controlling male sterility have been examined, and their applications in plant breeding have been evaluated.…”

Section: Introductionmentioning

confidence: 99%

“…Since the first report of a male sterile mutant in tomatoes in 1915, over 55 male sterile alleles have been identified and categorized into three types: sporogenous, functional, and structural sterility [ 5 , 6 ]. Most tomato male sterile mutants are of the sporogenous type [ 7 ], with only a few having been cloned, such as SlMS10 and SlMS32 .Both of these encode a basic helix–loop–helix (bHLH) transcription factor [ 8 , 9 , 10 ] and are homologous to DYT1 and bHLH10/89/90 in Arabidopsis , respectively [ 8 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Candidate Gene Identification and Transcriptome Analysis of Tomato male sterile-30 and Functional Marker Development for ms-30 and Its Alleles, ms-33, 7B-1, and stamenless-2

Wei,

Li,

Cao

et al. 2024

IJMS

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Male sterility is a valuable trait for hybrid seed production in tomato (Solanum lycopersicum). The mutants male sterile-30 (ms-30) and ms-33 of tomato exhibit twisted stamens, exposed stigmas, and complete male sterility, thus holding potential for application in hybrid seed production. In this study, the ms-30 and ms-33 loci were fine-mapped to 53.3 kb and 111.2 kb intervals, respectively. Tomato PISTILLATA (TPI, syn. SlGLO2), a B-class MADS-box transcription factor gene, was identified as the most likely candidate gene for both loci. TPI is also the candidate gene of tomato male sterile mutant 7B-1 and sl-2. Allelism tests revealed that ms-30, ms-33, 7B-1, and sl-2 were allelic. Sequencing analysis showed sequence alterations in the TPI gene in all these mutants, with ms-30 exhibiting a transversion (G to T) that resulted in a missense mutation (S to I); ms-33 showing a transition (A to T) that led to alternative splicing, resulting in a loss of 46 amino acids in protein; and 7B-1 and sl-2 mutants showing the insertion of an approximately 4.8 kb retrotransposon. On the basis of these sequence alterations, a Kompetitive Allele Specific PCR marker, a sequencing marker, and an Insertion/Deletion marker were developed. Phenotypic analysis of the TPI gene-edited mutants and allelism tests indicated that the gene TPI is responsible for ms-30 and its alleles. Transcriptome analysis of ms-30 and quantitative RT-PCR revealed some differentially expressed genes associated with stamen and carpel development. These findings will aid in the marker-assisted selection for ms-30 and its alleles in tomato breeding and support the functional analysis of the TPI gene.

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Genic male and female sterility in vegetable crops

Cited by 14 publications

References 158 publications

MADS-Box Family Genes in Lagerstroemia indica and Their Involvement in Flower Development

MADS-Box Family Genes in Lagerstroemia indica and Their Involvement in Flower Development

Onion: Breeding and Genomics

Candidate Gene Identification and Transcriptome Analysis of Tomato male sterile-30 and Functional Marker Development for ms-30 and Its Alleles, ms-33, 7B-1, and stamenless-2

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