Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway

Kim, Byung Chul; Kim, Hong Gyum; Lee, Sin Ae; Lim, Seunghwan; Park, Eun Hee; Kim, Seong‐Jin; Lim, Chang‐Jin

doi:10.1016/j.bcp.2005.07.025

Cited by 138 publications

(95 citation statements)

References 51 publications

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“…Stravopodis et al [32] reported the dose-dependent attenuation of p63 expression in RT4 cells at the mRNA level in doxorubicin-induced apoptosis. This result [13] . It has also been reported that JNK plays an important role in IQDMA-mediated G 2 /M arrest and apoptosis of K562 cancer cells [33] .…”

Section: Discussionmentioning

confidence: 64%

“…Genipin has been reported to induce apoptosis in FaO rat hepatoma cells, human hepatocarcinoma Hep3B cells and PC3 human prostate cancer cells [13,14] . However, the exact mechanism of genipin-induced apoptosis has not yet to be determined.…”

Section: Discussionmentioning

confidence: 99%

“…Although JNK and p38 MAPKs are collectively termed stress-activated protein kinases and are activated by a variety of stress-related stimuli and chemotherapy drugs [15,16] , there was no obvious change in p-p38 ( Figure 7C) in our studies. Kim et al [13] reported that the phosphorylation of JNK was clearly detectable in genipin-treated FaO cells, whereas MEK1/2 and p38 MAPKs were not activated after genipin treatment. Here, genipin dose-dependently upregulated the phosphorylation levels of JNK in K562 cells ( Figure 7A).…”

Section: Discussionmentioning

confidence: 99%

“…Notably, genipin has been shown to induce dosedependent apoptosis in FaO rat hepatoma cells, human hepatocarcinoma Hep3B cells [13] and PC3 human prostate cancer cells [14] . However, whether genipin shows a potential effect on human leukemia K562 cells and the cellular signaling pathway involved in this potential effect have not been well elucidated.…”

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confidence: 99%

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Apoptosis induced by genipin in human leukemia K562 cells: involvement of c-Jun N-terminal kinase in G2/M arrest

Qian

Cao

et al. 2011

Acta Pharmacol Sin

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Apoptosis induced by genipin in human leukemia K562 cells: involvement of c-Jun N-terminal kinase in G2/M arrest

Qian

Cao

et al. 2011

Acta Pharmacol Sin

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“…Genipin exerts anti-inflammatory, anti-angiogenic, antiproliferative and hepatoprotective activities in several cell lines including murine macrophage cells, human umbilical vein endothelial cells, human prostate cancer cells, and human hepatocarcinoma cells (2)(3)(4)(5). Genipin has been shown to inhibit growth of human leukemia and prostate cancer cells (5,6).…”

Section: Introductionmentioning

confidence: 99%

Genipin, a constituent of Gardenia jasminoides Ellis, induces apoptosis and inhibits invasion in MDA-MB-231 breast cancer cells

Kim

Jeong²,

Moon

2011

Oncol Rep

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Abstract. Genipin, a constituent of Gardenia jasminoides Ellis, is used in the treatment of hepatic disorders and inflammatory diseases in traditional medicine. Although mounting evidence suggests an anti-tumor activity of genipin in several cancer cell systems, the inhibitory effect of genipin on the growth of breast cancer cells has not been reported yet. The present study aimed to investigate the anti-proliferative activity of genipin in MDA-MB-231 human breast cancer cells. Herein, we showed that genipin efficiently induced apoptosis in MDA-MB-231 cells by the down-regulation of Bcl-2, up-regulation of Bax and proteolytic activation of caspase-3. Activation of JNK and p38 MAPK also increased by genipin. Importantly, genipin significantly inhibited invasive and migratory phenotypes of MDA-MB-231 cells. Taken together, this study demonstrates that genipin induces apoptosis and inhibits invasive/migratory abilities of highly invasive MDA-MB-231 human breast cancer cells, suggesting a potential application of genipin as a chemopreventive agent that may prevent or alleviate metastatic breast cancer.

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Simultaneous determination of geniposide and its metabolites genipin and genipinine in culture of Aspergillus niger by HPLC

Chen

Han

Zhang

et al. 2008

Biomedical Chromatography

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When cultivated with Aspergillus niger, geniposide, an important drug, is transformed into genipin and genipinine. A simple and rapid HPLC method for simultaneous determination of geniposide and its two metabolites in broth of A. niger is described. The chromatographic separation was achieved on a C18 ODS column (250 x 4.6 mm) by gradient elution with 0.1% formic acid in water and 0.1% formic acid in acetonitrile as the gradient mixtures. The flow rate was 1 mL/min, the detection wavelength was 238 nm and the column temperature was kept at 28 degrees C. The retention times of geniposide, genipin and genipinine were 10.9, 13.8 and 21.5 min, respectively. The mean absolute recoveries of three analysts were over 98%. Quantification limits were 0.01 microg/mL for geniposide and 0.02 microg/mL for the two metabolites. The method was applied for the quantification of geniposide, genipin and genipinine during fermentation and the evaluation of the bioavailabilities of these three compounds in Caco-2 monolayer.

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Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway

Cited by 138 publications

References 51 publications

Apoptosis induced by genipin in human leukemia K562 cells: involvement of c-Jun N-terminal kinase in G2/M arrest

Apoptosis induced by genipin in human leukemia K562 cells: involvement of c-Jun N-terminal kinase in G2/M arrest

Genipin, a constituent of Gardenia jasminoides Ellis, induces apoptosis and inhibits invasion in MDA-MB-231 breast cancer cells

Simultaneous determination of geniposide and its metabolites genipin and genipinine in culture of Aspergillus niger by HPLC

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