Genistein in the control of breast cancer cell growth: insights into the mechanism of action in vitro

Fioravanti, L.; Cappelletti, Vera; Miodini, Patrizia; Ronchi, E.; Brivio, M.; Fronzo, G. Di

doi:10.1016/s0304-3835(98)00130-x

Cited by 101 publications

(73 citation statements)

References 22 publications

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“…It is also possible that the effects of phytoestrogens may be manifested at the level of protein kinase activity in the prostate. It is known that genistein decreases tyrosine phosphorylation in endocrine tissue (Fioravanti et al 1998). Finally, the decrease in testosterone levels in the Phyto-600 animals remains to be explained but represents a consistent observation (Sharma et al 1992, Landstrom et al 1998.…”

Section: Discussionmentioning

confidence: 76%

Dietary soy-phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5alpha-reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats

Weber

Setchell²,

Stocco³

et al. 2001

Journal of Endocrinology

191

135

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Nutritional factors, especially phytoestrogens, have been extensively studied for their potential beneficial effects against hormone-dependent and age-related diseases. The present study describes the short-term effects of dietary phytoestrogens on regulatory behaviors (food/water intake, locomotor activity and body weight), prostate weight, prostate 5 -reductase enzyme activity, reproductive hormone levels, and testicular steroidogenic acute regulatory peptide (StAR) levels in adult Sprague-Dawley rats.Animals were fed either a phytoestrogen-rich diet containing ]600 µg/g isoflavones (as determined by HPLC) or a phytoestrogen-free diet. After 5 weeks of consuming these diets, plasma phytoestrogen levels were 35 times higher in animals fed the phytoestrogen-rich vs phytoestrogen-free diets. Body and prostate weights were significantly decreased in animals fed the phytoestrogenrich diet vs the phytoestrogen-free fed animals; however, no significant change in prostate 5 -reductase enzyme activity was observed between the treatment groups. Locomotor activity levels were higher in the phytoestrogen-rich vs the phytoestrogen-free animals during the course of the treatment interval. Plasma testosterone and androstenedione levels were significantly lower in the animals fed the phytoestrogen-rich diet compared with animals fed the phytoestrogen-free diet. However, there were no significant differences in plasma LH or estradiol levels between the diet groups. Testicular StAR levels were not significantly different between the phytoestrogen-rich vs the phytoestrogen-free fed animals.These results indicated that consumption of dietary phytoestrogens resulting in very high plasma isoflavone levels over a relatively short period can significantly alter body and prostate weight and plasma androgen hormone levels without affecting gonadotropin or testicular StAR levels.The findings of this study identify the biological actions of phytoestrogens on male reproductive endocrinology and provide insights into the protective effects these estrogen mimics exert in male reproductive disorders such as benign prostatic hyperplasia and prostate cancer.

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Section: Discussionmentioning

confidence: 76%

Dietary soy-phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5alpha-reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats

Weber

Setchell²,

Stocco³

et al. 2001

Journal of Endocrinology

191

135

View full text Add to dashboard Cite

show abstract

“…This value corresponds with our data. Considerable attention has been focused on the estrogenic effects of genistein (14,16,33,34). However, much less is known about the effect of daidzein and glycitein, which are present in significant quantities in soy products (35,36).…”

Section: Discussionmentioning

confidence: 99%

Optimization of a yeast estrogen screen and its applicability to study the release of estrogenic isoflavones from a soygerm powder.

Boever

Demaré

Vanderperren

et al. 2001

Environ Health Perspect

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Here we describe a redesigned protocol of the yeast estrogen screen developed by Routledge and Sumpter. The redesigned test comprises two steps. First, a large amount of yeast with estrogenic compounds is incubated for 24 hr. Subsequently, a mixture of cycloheximide and the chromogenic substrate chlorophenol red-beta-d-galactopyranoside (CPRG) is added. The cycloheximide stops protein synthesis and allows for an end-point measurement of beta-galactosidase activity generated during the first 24 hr. CPRG is converted to chlorophenol red and reflects beta-galactosidase activity, which is indicative of the estrogenic activity. The modifications shorten the duration of the assay at least 1 day and avoid interference of the estrogenic CPRG or chlorophenol red. The redesigned and the original protocol were used to study the estrogenic activity of bisphenol A, methoxychlor, p,p'-DDT, and isoflavones (genistein, daidzein, and glycitein). Bisphenol A, methoxychlor, and genistein triggered higher levels of beta-galactosidase activity in the redesigned protocol. Estrogenic activity of p,p'-DDT could only be demonstrated with the redesigned protocol. Glycitein and daidzein failed to give a response with both protocols. We also studied deconjugation of beta-glycosidic isoflavones present in soygerm powder. Treatment of the soygerm powder with beta-glycosidase released isoflavones. The estrogenic response of the samples was confirmed with the redesigned protocol and correlated with the amount of genistein present. The release of isoflavones under conditions prevailing in the intestines was studied. Bacterial beta-glycosidase present in the large intestine released isoflavones, and moderate estrogenic activity could be demonstrated.

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“…In addition, vitamin D 3 inhibits prostate adenocarcinoma growth and metastasis in the Dunning rat prostate model system (10) and displays anti-neoplastic activity in a variety of human xenograft tumor model systems (11,12). Several vitamin D 3 analogues have also been developed and display similar activities in vitro (5,(13)(14)(15) and in vivo (16 -18). Furthermore, we have demonstrated that vitamin D 3 inhibits the proliferation of squamous cell carcinoma (SCC) cells in vitro as well as the growth of newly transplanted or established SCC tumors in vivo (19,20).…”

Section: 25-dihydroxycholecalciferol (Vitamin D 3 )mentioning

confidence: 99%

Vitamin D3-induced Apoptosis of Murine Squamous Cell Carcinoma Cells

McGuire¹,

Trump²,

Johnson³

2001

Journal of Biological Chemistry

125

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Genistein in the control of breast cancer cell growth: insights into the mechanism of action in vitro

Cited by 101 publications

References 22 publications

Dietary soy-phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5alpha-reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats

Dietary soy-phytoestrogens decrease testosterone levels and prostate weight without altering LH, prostate 5alpha-reductase or testicular steroidogenic acute regulatory peptide levels in adult male Sprague-Dawley rats

Optimization of a yeast estrogen screen and its applicability to study the release of estrogenic isoflavones from a soygerm powder.

Vitamin D3-induced Apoptosis of Murine Squamous Cell Carcinoma Cells

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