Genistein induces apoptosis and topoisomerase II-mediated DNA breakage in colon cancer cells

Salti, George I.; Grewal, Seema; Mehta, Rajendra; Gupta, Tapas K. Das; Boddie, Arthur W.; Constantinou, Andreas I.

doi:10.1016/s0959-8049(00)00017-4

Cited by 116 publications

(60 citation statements)

References 14 publications

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“…In HT-29 cells, a 4-d exposure to 60 and 150 M of genistein resulted in 54 and 94% apoptotic cells, respectively (47). We assessed apoptosis by detecting active caspase-3, a key protease in the execution of apoptosis and an early marker of apoptosis (32), through immunohistochemistry and found active caspase-3 localized to enterocytes mainly at the tips of the villi, where it has also been reported by other laboratories (48,49).…”

supporting

confidence: 54%

Genistein Inhibits Intestinal Cell Proliferation in Piglets

et al. 2005

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supporting

confidence: 54%

Genistein Inhibits Intestinal Cell Proliferation in Piglets

et al. 2005

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“…In cell populations, the green cells (excluding propidium iodide) are viable with diffused chromatin, and those with condensed chromatin are apoptotic. The red cells (including propidium iodide) with noncondensed chromatin are necrotic ( Figure 1A) (Salti et al, 2000). A significant reduction in apoptosis was observed in neutrophils cocultured with supernatants of MSCs treated with 100 and 200 nM of calcitriol for 48 h and/or 72 h compared with supernatants of the control group (supernatants of MSCs that were not pulsed with calcitriol) ( Figure 1B).…”

Section: Resultsmentioning

confidence: 97%

Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions

Galeh¹,

Delirezh²,

Froushani³

et al. 2014

Turk J Biol

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IntroductionComplicated crosstalk between environmental factors and multiple genes determines which individuals will develop any given immune-mediated disease (Cantorna, 2010). Calcitriol [1α-25(OH)-vitamin D3] is one of the steroid hormone families and, similar to other members of these families, participates in the regulation of gene expression (Cantorna, 2010;Smyk et al., 2013). On the other hand, calcitriol may be an environmental factor that contributes to immune-mediated disease development. Environmental sources of calcitriol include diet and production in the skin following UV exposure to precursor 7-dehydrocholesterol (Namgung et al., 1994).Bone-marrow-derived mesenchymal stem cells (MSCs) are multipotent and can give rise to mesenchymal tissues like bone, cartilage, and fat (Uccelli et al., 2008). They also have potent immunomodulatory properties and may be valuable tools for cell-based immunotherapy (Meirelles Lda et al., 2009;Ghannam et al., 2010;Zhang et al., 2013). MSCs in bone marrow and tissue form a niche that has inevitable interactions with hematopoietic cells including neutrophils (Raffaghello et al., 2008;Maqbool et al., 2011). Neutrophils are one of the major cell types that constitute innate immunity. They predominate in host tissues during acute inflammatory processes (Greenberg and Grinstein, 2002).Recent documents have shown that calcitriol has an important role in regulating the growth of MSCs (Artaza et al., 2010;Klotz et al., 2012). The present study was carried out to investigate the effects of calcitriol on the interaction between bone-marrow-derived MSCs and neutrophils in rats. Materials and methods MaterialsPropidium iodide, acridine orange, and phosphatebuffered saline (PBS) were procured from Sigma-Aldrich (St Louis, MO, USA). May-Grünwald-Giemsa stain was purchased from Merck (Darmstadt, Germany) and dextran was obtained from Fresenius Kabi (Verona, Italy). Fetal calf serum, Dulbecco's Modified Eagle Medium (DEMEM), and RPMI 1640 were purchased from GIBCO/Life Technologies Inc. (Gaithersburg, MD, USA). The enzymelinked immunosorbent assay (ELISA) kit for interleukin (IL)-6 was purchased from Bender MedSystems

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“…Concerning cell cycle control, genistein has been demonstrated not only to inhibit topoisomerase II activity (11,12) but also receptor tyrosine kinases such as epidermal growth factor-receptor (EGFR) (6,31) or insulin-like growth factorreceptor type 1 (32). Bai et al (33) showed that genistein at a concentration of 10 M down-regulated gene expression of the EGFR signaling pathway in Panc 1 cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…Third, genistein has been shown to influence cell cycle progression in cultures of various growing cancer cell lines by blocking the cells during G 2 /M phase (7)(8)(9) and is therefore a significant inhibitor of in vitro cell growth (8,10). At high concentrations, genistein may also induce apoptosis (11) and cause DNA damage by directly influencing topoisomerase II activity (12).…”

mentioning

confidence: 99%

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

et al. 2008

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Soy-based formulas are consumed by growing numbers of infants and used as regular food supplements in livestock production. Moreover, constituent dietary phytoestrogens may compete with endogenous estrogens and affect individual growth. This study aimed to investigate the in vitro effects of isoflavones in comparison with estrogens on the proliferation of porcine satellite cells derived from neonatal muscle. After 7 h of exposure in serumfree medium, 17␤-estradiol (1 nM, 1 M), estrone (1 M), and daidzein (1, 100 M) slightly decreased whereas 100 M genistein substantially lowered DNA synthesis. Declines in DNA amount were observed with genistein (1, 100 M) and daidzein (100 M). After 26 h of exposure, 100 M genistein reduced DNA synthesis, whereas it was increased by 10 M genistein and 10 and 100 M daidzein. In the case of 10 M genistein and 100 M daidzein, these increases apparently resulted from the repair of damaged DNA. Genistein and daidzein (100 M) reduced protein synthesis, caused a G 2 /M phase block, and decreased DNA amount in association with higher rates of cell death partially resulting from apoptosis. Conclusively, isoflavones at concentrations of greater than 1 M act as inhibitors of porcine skeletal muscle cell proliferation.

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Genistein induces apoptosis and topoisomerase II-mediated DNA breakage in colon cancer cells

Cited by 116 publications

References 14 publications

Genistein Inhibits Intestinal Cell Proliferation in Piglets

Genistein Inhibits Intestinal Cell Proliferation in Piglets

Calcitriol modulates the effects of the supernatants of bone-marrow–derived mesenchymal stem cells on neutrophil functions

Effects of Dietary Isoflavones on Proliferation and DNA Integrity of Myoblasts Derived from Newborn Piglets

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