Genital human papillomavirus infection in female university students as determined by a PCR‐based method

Bauer, HM; Ting, Yi Tian; Greer, CE; Chambers, J C; Tashiro, CJ; Chimera, J A; Reingold, Arthur; Manos, MM

doi:10.1016/0020-7292(91)90514-6

Cited by 171 publications

(203 citation statements)

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“…We have used PCR technology since this is the most sensitive method of detecting HPV in cervical smears (Schiffman et al, 1991). By this method, complete congruence with the most prominent classical risk factors for cervical cancer, such as sexarche and promiscuity, has been shown (Bauer et al, 1991). Age dependence (Melkert et al, 1993) and a strong association with increased HPV prevalence in relation to CIN grade in cross-sectional studies have been observed (Wrincz et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

The presence of persistent high‐risk hpv genotypes in dysplastic cervical lesions is associated with progressive disease: Natural history up to 36 months

Remmink

Walboomers

Helmerhorst

et al. 1995

Intl Journal of Cancer

333

211

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To evaluate the clinical significance of HPV genotyping for the prediction of progressive cervical intraepithelial neoplasia (CIN) in women with cytomorphologically abnormal smears, a prospective, blind, non-intervention study was performed. A total of 342 patients screened with cytomorphologically abnormal cervical smears were monitored every 3-4 months by cervical cytology, colposcopy and HPV testing using PCR. Women with progressive CIN disease were defined as patients developing lesions with a colposcopic impression of CIN III over more than 2 quadrants or resulting in a cytological smear equivalent to Pap 5. These patients were subsequently treated according to standard procedures. If any doubt arose about the true status of the patients (n = 75) these patients were censored and biopsied. The mean follow-up time was 16.5 months (range 3-36 months). Nineteen women showed progressive CIN disease and all appeared to be continuously HPV-positive from the start of the study. At biopsy, all these patients were histologically classified as CIN III. Seventeen of these women were positive for high-risk HPV types. Two cases were classified as still unidentified HPV. No progression was seen in the absence of HPV DNA or in the presence of low-risk HPV types. In life-table analysis the cumulative rate of progressive, histologically verified CIN disease was 17% after 36 months. Further analyses showed that other risk factors such as age, sexarche, number of sexual partners or smoking hardly influenced the effect of HPV on progression. The results show that the continuous presence of high-risk HPV types in women with cytomorphologically abnormal smears is a strong marker for progressive CIN disease.

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Section: Discussionmentioning

confidence: 99%

The presence of persistent high‐risk hpv genotypes in dysplastic cervical lesions is associated with progressive disease: Natural history up to 36 months

Remmink

Walboomers

Helmerhorst

et al. 1995

Intl Journal of Cancer

333

211

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“…18 Briefly, presence of HPV-DNA was assessed using MY09/MY11/ HMB01-PCR system with Gold AmpliTaq that amplifies a conserved 450 base-pair segment in the L1-sequence of HPV. [19][20][21] To assess for HPV16 mRNA expression, total RNA was extracted from the same or matched frozen tumor samples and tested for HPV16 transcripts using HPV specific oligonucleotide primers that span the 204-525 base-pair regions of the E6 and E7 oncogenes. 22,23 HPV16 RNApositive tumors were defined as those that expressed either E6 or E7 transcripts.…”

Section: Hpv Dna and Hpv16 E6/e7 Mrna Detectionmentioning

confidence: 99%

Combined P16 and human papillomavirus testing predicts head and neck cancer survival

Salazar

Anayannis

Smith

et al. 2014

Intl Journal of Cancer

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While its prognostic significance remains unclear, p16INK4a protein expression is increasingly being used as a surrogate marker for oncogenic human papillomavirus (HPV) infection in head and neck squamous cell carcinomas (HNSCC). To evaluate the prognostic utility of p16 expression in HNSCC, we prospectively collected 163 primary tumor specimens from histologically confirmed HNSCC patients who were followed for up to 9.4 years. Formalin fixed tumor specimens were tested for p16 protein expression by immunohistochemistry. HPV type-16 DNA and RNA was detected by MY09/11-PCR and E6/E7 RT-PCR on matched frozen tissue, respectively. P16 protein expression was detected more often in oropharyngeal tumors (53%) as compared with laryngeal (24%), hypopharyngeal (8%), or oral cavity tumors (4%; P<0.0001). With respect to prognosis, p16-positive oropharyngeal tumors exhibited significantly better overall survival than p16-negative tumors (log-rank test p=0.04), whereas no survival benefit was observed for non-oropharyngeal tumors. However, when both p16 and HPV DNA test results were considered, concordantly positive non-oropharyngeal tumors had significantly better disease-specific survival than concordantly negative non-oropharyngeal tumors after controlling for sex, nodal stage, tumor size, tumor subsite, primary tumor site number, smoking, and drinking (adjusted hazard ratio [HR]=0.04, 0.01–0.54). Compared with concordantly negative non-oropharyngeal HNSCC, p16(+)/HPV16(-) non-oropharyngeal HNSCC (n=13, 7%) demonstrated no significant improvement in disease-specific survival when HPV16 was detected by RNA (adjusted HR=0.83, 0.22–3.17). Our findings show that p16 immunohistochemistry alone has potential as a prognostic test for oropharyngeal cancer survival, but combined p16/HPV testing is necessary to identify HPV-associated non-oropharyngeal HNSCC with better prognosis.

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“…5 In women younger than 25 years of age, HPV infection rates have been reported to range from 28% to 46%. 6,7 In the vast majority of cases, HPV infections are usually transient and do not necessarily lead to clinically significant lesions of the cervical mucosa. 8 Given the high incidence of HPV infection compared with the low prevalence of cervical cancer, other factors must be involved in the malignant transformation of the cervical mucosa.…”

mentioning

confidence: 99%

Human papillomavirus testing and molecular markers of cervical dysplasia and carcinoma

Dehn¹,

Torkko²,

Shroyer

2007

Cancer

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Genital human papillomavirus infection in female university students as determined by a PCR‐based method

Cited by 171 publications

References 0 publications

The presence of persistent high‐risk hpv genotypes in dysplastic cervical lesions is associated with progressive disease: Natural history up to 36 months

The presence of persistent high‐risk hpv genotypes in dysplastic cervical lesions is associated with progressive disease: Natural history up to 36 months

Combined P16 and human papillomavirus testing predicts head and neck cancer survival

Human papillomavirus testing and molecular markers of cervical dysplasia and carcinoma

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