Genome analysis of a G9P[23] group A rotavirus isolated from a dog with diarrhea in China

Yan, Nan; Tang, Cheng; Kan, Ruici; Feng, Fan; Yue, Hua

doi:10.1016/j.meegid.2019.02.020

Cited by 10 publications

(10 citation statements)

References 28 publications

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“…The evolutionary analysis revealed that rotavirus G1 and G3 strains were dominant before 2017, while after 2018 there was a surge of human G9 strain. The G9 strain, meanwhile, has been present and dominant in swine and dogs [ 51 , 54 ]. Previous studies have revealed that the G3 and G9 strains of RAV cause diarrhea in human infants and young children, and the recombination of RVA in humans and animals causes the generation of new PoRVA genotypes [ 55 , 56 , 57 , 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Geng

et al. 2022

Viruses

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Swine enteric viruses are a major cause of piglet diarrhea, causing a devastating impact on the pork industry. To further understand the molecular epidemiology and evolutionary diversity of swine enteric viruses, we carried out a molecular epidemiological investigation of swine enteric viruses (PEDV, PDCoV, PoRVA, and TGEV) on 7107 samples collected from pig farms in south-central China. The results demonstrated that PEDV is the predominant pathogen causing piglet diarrhea, and its infection occurs mainly in relatively cold winter and spring in Hunan and Hubei provinces. The positive rate of PEDV showed an abnormal increase from 2020 to 2021, and that of PoRVA and PDCoV exhibited gradual increases from 2018 to 2021. PEDV-PoRVA and PEDV-PDCoV were the dominant co-infection modes. A genetic evolution analysis based on the PEDV S1 gene and ORF3 gene revealed that the PEDV GII-a is currently epidemic genotype, and the ORF3 gene of DY2020 belongs to a different clade relative to other GII-a strains isolated in this study. Overall, our results indicated that the variant PEDV GII-a is the main pathogen of piglet diarrhea with a trend of outbreak. G9 is the dominant PoRVA genotype and has the possibility of outbreak as well. It is therefore critical to strengthen the surveillance of PEDV and PoRVA, and to provide technical reserves for the prevention and control of piglet diarrhea.

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Section: Discussionmentioning

confidence: 99%

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Geng

et al. 2022

Viruses

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“…In contrast, sequence variation among strains between lineages was considerably higher, ranging from 5% to 14% (Phan et al, 2007). The number of a novel sub‐lineage of G9 lineage III has increased continuously in recent years, and has become phylogenetically more complex (Yan et al, 2019). In this study, the three G9 RVAs were assigned to different lineages, and belonged to lineages III and VI.…”

Section: Discussionmentioning

confidence: 99%

“…The cDNA was stored at −20°C. To determine the viral genome sequences in the RVA‐positive samples, the complete sequences of the three porcine RVAs were amplified with RT–PCR using 27 pairs of primers (Yan et al, 2019). The PCR products were purified, cloned into the pMD19‐T vector (TaKaRa) and transformed into Escherichia coli competent cells DH5α for Sanger sequencing.…”

Section: Methodsmentioning

confidence: 99%

Genetic and immunological characterization of G9 group A porcine rotaviruses in China

Liu

Wang

Kan

et al. 2022

Zoonoses and Public Health

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G9 group A rotaviruses (RVAs) are considered emerging pathogens in pigs and humans, and pigs are considered a potential host reservoir for human G9 RVAs. In this study, RVAs of two genotypes, G9P[23] and G9P[13], were successfully isolated and the genomic sequences were obtained, the genome constellation is G9‐P[23]‐I5‐R1‐C1‐M1‐A8‐N1‐T1‐E1‐H1 and G9‐P[13]‐I5‐R1‐C1‐M1‐A8‐N1‐T7‐E1‐H1 respectively. One strain which amplified from clinic faecal sample had an unique genome constellation G9‐P[23]‐I1‐R1‐C1‐M1‐A8‐N1‐T1‐E1‐H1. All the genomic segments of three porcine G9 RVAs were closely related to those of porcine and/or porcine‐like human RVAs, demonstrating that the three viruses were porcine–human reassortant strains. To study the immunogenicity of the porcine G9 RVAs, 6‐week‐old female BALB/c mice were immunized with inactivated vaccines derived from porcine RVAs and then mated. The highest titres of neutralizing antibodies against G9P[23] and G9P[13] porcine RVAs (1,291 ± 35.22 and 1:232 ± 39.28 respectively) were produced in mice 7 days after the second immunization. Suckling mice born to the vaccinated dams were protected by maternal antibodies against challenge with homologous strains. Overall, our data demonstrate the occurrence of porcine–human reassortants of G9 RVAs, and extend our understanding of the immunogenicity of porcine G9 rotaviruses. They also provide a basis for the development of a porcine G9 RVA vaccine.

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“…The second example is that through the phylogenetic tree analysis of the genotypes of HRV in Wuhan from 2000 to 2013 [48], it was found that the G3P [8] type HRV was a RV from other species. The third example is the first successful isolation of the G9P [23] CRV strain from dog faeces by Yan and colleagues; the G9 genotype is widely prevalent in humans and pigs [2].…”

Section: Discussionmentioning

confidence: 99%

“…Rotavirus is the genus of the family Reoviridae with viruses that are a major cause of gastroenteritis in infants and young animals worldwide [1][2][3]. Rotavirus causes diarrhoea in many animals [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Molecular evolution and genetic characteristics of G3P[3] group A canine rotavirus isolated in Wuhan, China

Chen

et al. 2022

Journal of General Virology

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Rotaviruses can infect multiple animal species and have the potential for cross-recombination based on the segmented genome characteristics. To study the intra-host recombination and zoonotic potential of group A canine rotavirus (CRV), 438 samples were collected from domestic dogs in six animal hospitals and from stray dogs from October 2019 to May 2021 in Wuhan, China. Seven of the samples were positive (7/438) for group A CRV from which a CRV strain was successfully isolated in MA-104 cells. The genotype of the isolated strain was characterized by whole-genome sequencing showing that the genotype was group A CRV G3P[3]. According to the Rotavirus Classification Working Group (RCWG), the genomic constellation of the isolated CRV was G3-P[3]-I3-R3-C3-M3-A9-N2-T3-E3-H6, which belongs to the AU-1-like group with gene segments of AU-1-like and Cat 97-like strains. Based on the phylogenetic analysis of the 11 gene segments, we found that the different segments of the isolated group A CRV were closely related to several reassortment rotaviruses from different animal sources and bat strains. Based on the analysis of the molecular evolution and genetic characteristics, we concluded that the isolated strain might be a reassortment strain. These data further enrich our understanding of rotavirus molecular evolution and genetic characteristics in China.

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Genome analysis of a G9P[23] group A rotavirus isolated from a dog with diarrhea in China

Cited by 10 publications

References 28 publications

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Epidemic and Evolutionary Characteristics of Swine Enteric Viruses in South-Central China from 2018 to 2021

Genetic and immunological characterization of G9 group A porcine rotaviruses in China

Molecular evolution and genetic characteristics of G3P[3] group A canine rotavirus isolated in Wuhan, China

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