Genome analysis provides insights into crude oil degradation and biosurfactant production by extremely halotolerant Halomonas desertis G11 isolated from Chott El-Djerid salt-lake in Tunisian desert

Neifar, Mohamed; Chouchane, Habib; Najjari, Afef; Hidri, Darine El; Mahjoubi, Mouna; Ghedira, Kaïs; Naili, Fatma; Soufi, Leila; Raddadi, Noura; Sghaier, Haïtham; Ouzari, Hadda Imen; Masmoudi, Ahmed Slaheddine; Chérif, Ameur

doi:10.1016/j.ygeno.2018.12.003

Cited by 43 publications

(18 citation statements)

References 79 publications

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“…SZN1 and Alcanivorax sp. SZN2 belong to genera already described as capable of degrading hydrocarbons [31,32], they do not cluster closely with species with hydrocarbon degrading activity. Similarly, Pseudoalteromonas sp.…”

Section: Heavy Metal Immobilizationmentioning

confidence: 87%

Degradation of Hydrocarbons and Heavy Metal Reduction by Marine Bacteria in Highly Contaminated Sediments

et al. 2020

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Investigations on the ability of bacteria to enhance removal of hydrocarbons and reduce heavy metal toxicity in sediments are necessary to design more effective bioremediation strategies. In this study, five bacterial strains, Halomonas sp. SZN1, Alcanivorax sp. SZN2, Pseudoalteromonas sp. SZN3, Epibacterium sp. SZN4, and Virgibacillus sp. SZN7, were isolated from polluted sediments from an abandoned industrial site in the Gulf of Naples, Mediterranean Sea, and tested for their bioremediation efficiency on sediment samples collected from the same site. These bacteria were added as consortia or as individual cultures into polluted sediments to assess biodegradation efficiency of polycyclic aromatic hydrocarbons and heavy metal immobilisation capacity. Our results indicate that these bacteria were able to remove polycyclic aromatic hydrocarbons, with a removal rate up to ca. 80% for dibenzo-anthracene. In addition, these bacteria reduced arsenic, lead, and cadmium mobility by promoting their partitioning into less mobile and bioavailable fractions. Microbial consortia generally showed higher performance toward pollutants as compared with pure isolates, suggesting potential synergistic interactions able to enhance bioremediation capacity. Overall, our findings suggest that highly polluted sediments select for bacteria efficient at reducing the toxicity of hazardous compounds, paving the way for scaled-up bioremediation trials.

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Section: Heavy Metal Immobilizationmentioning

confidence: 87%

Degradation of Hydrocarbons and Heavy Metal Reduction by Marine Bacteria in Highly Contaminated Sediments

et al. 2020

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“…Genomic DNA extraction of TL13 strain was performed by sodium dodecyl sulfate–proteinase K treatment (Neifar et al, 2019 ). Purified genomic DNA was sequenced on an Illumina MiSeq platform (MRDNA, USA) yielding 2 ×250 bp paired-end reads.…”

Section: Methodsmentioning

confidence: 99%

New Plant Growth-Promoting, Chromium-Detoxifying Microbacterium Species Isolated From a Tannery Wastewater: Performance and Genomic Insights

Ouertani

Ouertani²,

Mahjoubi³

et al. 2020

Front. Bioeng. Biotechnol.

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Hexavalent chromium [Cr(VI)], widely generated by tannery activities, is considered among the most toxic substances and causes a serious damage for the environment and for human health. Interestingly, some microorganisms have a potential of bioremediation of chromium-contaminated wastewaters and soils through the reduction of Cr(VI) (soluble and harmful form) into Cr(III) (stable and non-toxic form). Here, we present the full genome sequence of a novel heavy-metal-resistant, plant growth-promoting bacterium (PGPB), Microbacterium metallidurans TL13, which was isolated from a Tunisian leather industry. The strain TL13 was resistant to many heavy metals, such as chromium, copper, nickel, cobalt, and arsenic. The 50% TL13 growth inhibitory concentration (IC 50) values of HgCl

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“…The larger the magnitude of t-value and smaller the p-value indicate the high significance of the corresponding model coefficient. 21,28 It can be seen from Table 3 that the linear effect of the parameters S1, S2 and S3 and the bacterial strain interactions S1S2, S1S3 and S2S3 were statistically significant. Furthermore, the variables with the largest significant effect (p≤ 0.001) were the linear term of S1, S2 and S3, as well as the squared term of S1S2.…”

Section: Statistical Analysis Of the Modelsmentioning

confidence: 95%

“…These plant growth-promoting, bioremediating strains were previously isolated from extreme environmental sites, and taxonomically identified by 16S rRNA gene sequence analysis and stored in cryovials with 20% (v/v) glycerol solution at -80°C until use. 21,22 The strains were reactivated on Nutrient Agar plates at 30°C for 48 hr. An antagonism test was carrying out to prove the synergism between strains to facilitate the development of consortium.…”

Section: Dye Media and Chemicalsmentioning

confidence: 99%

Application of a mixture design to optimize textile azo-dye decolorization using a bacterial consortium

Sghaier¹,

Ouertani²,

Mahjoubi³

et al. 2019

BBIJ

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Textile wastewaters (TWWs) are characterized by high salinity and alkaline pH. Bioremediation using fungi were proved in many cases as inefficient tools to treat such effluent, giving the relay to haloalkaliphilic bacteria. Here, three extremophilic strains namely Halomonas desertis G11, Kocuria rosea BU22S and Microbaterium trichothecenolyticum TL13 have been selected to conduct textile dye decolorization experiments. The effect of different combinations of these strains was studied by a mixture design (MD) to assess Tubantin Brown GGL (TB GGL) color removal during species growth under optimzed conditions of dye concentration (100 mg/L), pH (9), salinity (5%), inoculum size (5%) and time (10 days). A remarkable decolorization was observed using mono and mixed cultures. Using the NemrodW software, the optimisation calculations were performed to find an optimum mixture proportions for maximum azo dye decolorization. High regression coefficients R2, between the variables and the response indicated excellent evaluation of experimental data by the polynomial regression model. The highest color removal (about 92%) was obtained with binary mixture composed by H. desertis G11and M. trichothecenolyticum TL13 and it was in close agreement with the estimated response value (93%). This finding shows a biotechnological potential of haloalkaliphilic bacteria in TWWs treatment.

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Genome analysis provides insights into crude oil degradation and biosurfactant production by extremely halotolerant Halomonas desertis G11 isolated from Chott El-Djerid salt-lake in Tunisian desert

Cited by 43 publications

References 79 publications

Degradation of Hydrocarbons and Heavy Metal Reduction by Marine Bacteria in Highly Contaminated Sediments

Degradation of Hydrocarbons and Heavy Metal Reduction by Marine Bacteria in Highly Contaminated Sediments

New Plant Growth-Promoting, Chromium-Detoxifying Microbacterium Species Isolated From a Tannery Wastewater: Performance and Genomic Insights

Application of a mixture design to optimize textile azo-dye decolorization using a bacterial consortium

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