Genome based safety assessment for Bacillus coagulans strain LBSC (DSM 17654) for probiotic application

Saroj, Dina B; Gupta, Anil Kumar

doi:10.1016/j.ijfoodmicro.2020.108523

Cited by 52 publications

(23 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, we inferred that there was a low associated risk of resistance genes [tet (L) and cfr] transfer due to the absence of mobile elements within the gene vicinity. The WLYS23 strain is unlikely to mediate transferrable antibiotic resistance genes (Saroj and Gupta 2020). Therefore, genome-based safety assessment for the WLYS23 strain suggests that this strain is likely non-pathogenic to animals/fish.…”

Section: Discussionmentioning

confidence: 99%

Bacillus velezensis WLYS23 strain possesses antagonistic activity against hybrid snakehead bacterial pathogens

et al. 2021

View full text Add to dashboard Cite

Aim:The aims of this study were to screen an antagonistic probiotic for the prevention and control of bacterial diseases in snakehead fish and to evaluate the antimicrobial activities, biosafety and biocontrol effect of the antagonistic strain. Methods and Results: In this study, the WLYS23 strain exhibiting the most effective antagonistic properties against several fish pathogens was selected from the intestine of healthy snakehead fish. The strain was identified as Bacillus velezensis based on morphological, physiological and biochemical characteristics, and phylogenetic analysis. This strain showed amylase, cellulase, protease and lipase activities according to extracellular enzyme activity assays. Moreover, the cell-free extract of the WLYS23 strain exhibited strong antibacterial activity, with MICs of ≤64 lg ml À1 for most fish pathogens. Additionally, the cell-free extract was heat and pH stable, and resistant to protease, whereas their antimicrobial activities were significantly influenced by metal ions at high concentration. The WLYS23 strain and its cell-free extract were safe for snakehead and zebrafish. The disease resistance of snakehead fish was significantly increased following administration of the WLYS23 strain and its cell-free extract respectively. The complete genome of the WLYS23 strain was sequenced and found to harbour seven gene clusters encoding secondary metabolites with antimicrobial activity. Conclusion:The WLYS23 strain possesses great potential as a biocontrol agent, which can be commercially developed to improve disease control in freshwater aquaculture. Significance and Impact of the Study: Snakehead fish are important commercially farmed fish worldwide. However, the bacterial diseases caused by Aeromonas sp. and Nocardia seriolae in farmed snakehead fish lead to huge economic losses. Safe, economical and efficient probiotics are limited to prevent and control these diseases. Here, we provide a promising biocontrol agent with antagonistic activity against bacterial diseases of snakehead.

show abstract

Section: Discussionmentioning

confidence: 99%

Bacillus velezensis WLYS23 strain possesses antagonistic activity against hybrid snakehead bacterial pathogens

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Antibiotic resistance genes and virulence genes were identified by local BLASTP with reference to the Comprehensive Antibiotic Resistance Database (CARD, , Version 1.1.3, accessed on 19 April 2021) and the virulence factor database (VFDB, , Version 1.0, accessed on 19 April 2021), respectively. We only considered BLAST results exhibiting more than 30% identity and 70% coverage and used a cut-off e-value of less than 0.01 [ 24 , 25 ]. IslandViewer ( , Version 4.0, accessed on 19 April 2021) was used for the identification and visualization of possible genomic islands in the DSM 2950 genome to assess its potential pathogenicity [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

et al. 2021

View full text Add to dashboard Cite

In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.

show abstract

“…The susceptibility of the strains to 8 clinically important antibiotics [chloramphenicol, clindamycin, erythromycin, ampicillin, kanamycin, streptomycin, tetracycline and ciprofloxacin: selected based on the EFSA guidelines for testing the antimicrobial susceptibility of the Bacillus species (EFSA 2012)] (Dina et al 2020). The strains (Kanjan and Sakpetch 2020) were tested for hemolytic activity by streaking on blood agar (5% v/v sheep blood).…”

Section: Safety Assessment Of the Strainsmentioning

confidence: 99%

Novel starter cultures Virgibacillus spp. selected from grasshopper sub shrimp paste to inhibit biogenic amines accumulation

et al. 2021

View full text Add to dashboard Cite

Controlling the content of biogenic amines (BAs) is critical to guarantee the safety of fermented aquatic products. The degradation characteristics and application potential of amine-negative starter cultures (Virgibacillus halodenitrificans CGMCC 1.18601: G25, Virgibacillus pantothenticus CGMCC 1.18602: G38) screened from grasshopper sub shrimp paste (Gssp) were studied. The enzymes of the two strains G25 and G38 that degrade BAs were amine oxidases (AOs) located on their respective cell membranes. The conditions that promoted the AO activity of Virgibacillus spp. were NaCl concentrations 5–10%, temperature 37 °C, pH 7.0 and ethanol concentrations 0–2%. Safety assessments (antibiotic susceptibility, biofilm activity and hemolytic activity) indicated that Virgibacillus spp. do not present a risk to human health, and this isolate can be confidently recommended as safe starter cultures for the food industry. Then, the two strains were cultured separately as starters and applied to the Gssp to analyze their influence on the flavor and quality of the product. As far as the bad flavors in Gssp such as sulfur-organic and sulf-chlor were concerned, the response values in the starter groups by G25 and G38 were significantly reduced by 39% and 65%, respectively. For the ability of strains to degrade BAs in Gssp, G25 degraded 11.1% of histamine, 11.3% of tyramine, 15.5% of putrescine and 4.1% of cadaverine; G38 significantly degraded 10.1% of histamine, 21.8% of tyramine, 18.1% of putrescine and 5.0% of cadaverine. These results indicated that the selected species could be used as starter cultures for the control of BA accumulation and degradation in Gssp.

show abstract

Genome based safety assessment for Bacillus coagulans strain LBSC (DSM 17654) for probiotic application

Cited by 52 publications

References 39 publications

Bacillus velezensis WLYS23 strain possesses antagonistic activity against hybrid snakehead bacterial pathogens

Bacillus velezensis WLYS23 strain possesses antagonistic activity against hybrid snakehead bacterial pathogens

A Comprehensive Assessment of the Safety of Blautia producta DSM 2950

Novel starter cultures Virgibacillus spp. selected from grasshopper sub shrimp paste to inhibit biogenic amines accumulation

Contact Info

Product

Resources

About