Genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus Botrytis cinerea

Wu, Mingde; Zhang, Lei; Li, Guoqing; Jiāng, Dàohóng; Ghabrial, Said A.

doi:10.1016/j.virol.2010.07.010

Cited by 142 publications

(134 citation statements)

References 48 publications

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“…Nevertheless, the differences in growth among isolates may be also having been due to a genetic influence (Zharare et al, 2010) a possibility not tested here due to not working with genetically similar strains. Previous studies have shown that viruses found in many fungi, e.g., Cryphonectria parasitica, Fusarium graminearum or Botrytis cinerea, produce several phenotypic changes such as reduction in growth and sporulation of the fungal strains they infect (Chu et al, 2002;Boland, 2004;Van Diepeningen et al, 2006;Robin et al, 2010;Wu et al, 2010;Zhang et al, 2010). In our study, the isolates growing at osmotic potential medium of -0.6 MPa and -1.8 MPa also showed a reduction of the mycelial growth.…”

Section: Discussionsupporting

confidence: 62%

“…Mitoviruses have been recorded in several phytopathogenic fungi such as Cryphonectria parasitica (Polashock and Hillman, 1994;Polashock et al, 1997), Ophiostoma novo-ulmi (Brasier, 1983;Rogers et al, 1987), Sclerotina homoeocarpa (Deng et al, 2003;Deng and Boland, 2004), Helicobasidium mompa (Osaki et al, 2005), Chalara elegans (Park et al, 2006) and Botrytis cinerea (Castro et al, 2003;Wu et al, 2007). In most cases, the presence of mitoviruses is associated with reduction of fungal pathogenicity (Ghabrial and Suzuki, 2009;Wu et al, 2010). Members of the genus Mitovirus have also been isolated in G. abietina (Tuomivirta and Hantula, 2003) which, in the Spanish population, has recently been discovered to host (Botella et al, 2012a).…”

Section: Resumen Efecto De Posibles Mitovirus En El Crecimiento In VImentioning

confidence: 99%

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Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

2012

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Mitoviruses have been found in several forest pathogens (i.e. Cryphonectria parasitica, Gremmeniella abietina), and because they have been shown to reduce the virulence of host fungi there is a growing interest in studying their use as a biocontrol. This study was carried out to test the effect of temperature (5°C, 15°C, 25°C and 35°C), pH (4, 5, 7 and 9) and osmotic potential (-0.6, -1.2, -1.8 and -2.4 MPa) on the mycelial growth of seven G. abietina isolates under controlled laboratory conditions. Four of the isolates hosted mitoviruses and three of them did not. During the experiment, mycelial growth was recorded every week for a period of 8 weeks. Results showed no differences in growth behavior between mitovirus infected and non-infected isolates when placed under different pH modifications. However, the mitovirus-infected isolates presented larger mycelial growth than the mitovirus-free ones when at the fungi's optimal growing temperature of 15°C. When growing at certain osmotic potentials (-0.6 and -1.8 MPa) a reduction in growth of the mitovirus-infected isolates was observed. The results of this experiment suggest that mycelial growth among non-infected isolates and isolates naturally infected by mitovirus vary under different culture conditions, thus providing further insight into the effects of mitovirus on Gremmeniella abietina isolates.Key words: mitoviruses; Scleroderris canker; in vitro; biological control; Gremmeniella abietina; dsRNA. Resumen Efecto de posibles mitovirus en el crecimiento in vitro de aislados de Gremmeniella abietina bajo diferentes condiciones de laboratorioLos mitovirus son virus exclusivamente fúngicos que han sido aislados de algunos patógenos forestales (i.e. Cryphonectria parasitica, Gremmeniella abietina) y ya que pueden reducir la virulencia del hongo existe un creciente interés por su posible papel como agentes de control biológico. Se ha llevado a cabo un estudio para evaluar el efecto de la temperatura (5°C, 15°C, 25°C y 35°C), el pH (4, 5, 7 y 9) y el potencial osmótico (-0.6, -1.2, -1.8, -2.4 MPa) en el crecimiento micelial de siete aislados de G. abietina bajo condiciones controladas de laboratorio. Cuatro de los aislados albergaban mitovirus y tres de ellos no. Durante el experimento, el crecimiento micelial fue registrado semanalmente hasta completar 8 mediciones. Los aislados infectados con mitovirus presentaron mayor crecimiento micelial que los no infectados a la temperatura de crecimiento óptimo del hongo de 15°C. No se observaron efectos de la presencia de mitovirus entre los aislados infectados y los no infectados en los tratamientos de modificación del pH. Cuando se modificaron los potenciales osmóticos se observó una reducción del crecimiento micelial de los aislados infectados con mitovirus en los potenciales osmóticos de -0.6 y -1.8 MPa. Los resultados de este experimento sugieren que la presencia de los mitovirus afecta al crecimiento micelial del hongo bajo distintas condiciones de laboratorio. Este estudio proporciona un conocimiento más prof...

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Section: Discussionsupporting

confidence: 62%

Section: Resumen Efecto De Posibles Mitovirus En El Crecimiento In VImentioning

confidence: 99%

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

2012

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“…Depressed production of conidia has been observed for hypovirus-infected strains of C. parasitica (51) and mycovirus-infected strains of Diaporthe ambigua (52). In addition, previous studies indicated that conidium production was reduced significantly in mycovirus-associated, hypovirulent strains of B. cinerea (53,54). Unexpectedly, we found that although wild-type strain KY-1 produced conidia in a normal fashion, SsPV1-infected strains KY-1V1 and KY-1V2 of B. cinerea produced more conidia than did strain KY-1.…”

Section: Discussionmentioning

confidence: 94%

“…Recently, B. cinerea was ranked by fungal pathologists as being the second most important pathogenic fungus based on scientific/economic importance in the journal Molecular Plant Pathology (56). Some hypovirulence-associated mycoviruses from B. cinerea and its related species Botrytis porri have been isolated and characterized (10,54,57), but these Botrytis viruses are unlikely to be used for control of diseases caused by these fungi under field conditions because they are easy to be eliminated via asexual propagation (see below). Expansion of the host range of mycoviruses has been accomplished for many fungus-mycovirus systems (7,8,24,(58)(59)(60)).…”

Section: Discussionmentioning

confidence: 99%

A Novel Partitivirus That Confers Hypovirulence on Plant Pathogenic Fungi

Xiao

Chéng

Tang

et al. 2014

J Virol

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Members of the family Partitiviridae have bisegmented double-stranded RNA (dsRNA) genomes and are not generally known to cause obvious symptoms in their natural hosts. An unusual partitivirus, Sclerotinia sclerotiorum partitivirus 1 (SsPV1/WF-1), conferred hypovirulence on its natural plant-pathogenic fungal host, Sclerotinia sclerotiorum strain WF-1. Cellular organelles, including mitochondria, were severely damaged. Hypovirulence and associated traits of strain WF-1 and SsPV1/WF-1 were readily cotransmitted horizontally via hyphal contact to different vegetative compatibility groups of S. sclerotiorum and interspecifically to Sclerotinia nivalis and Sclerotinia minor. S. sclerotiorum strain 1980 transfected with purified SsPV1/WF-1 virions also exhibited hypovirulence and associated traits similar to those of strain WF-1. Moreover, introduction of purified SsPV1/WF-1 virions into strain KY-1 of Botrytis cinerea also resulted in reductions in virulence and mycelial growth and, unexpectedly, enhanced conidial production. However, virus infection suppressed hyphal growth of most germinating conidia of B. cinerea and was eventually lethal to infected hyphae, since very few new colonies could develop following germ tube formation. Taken together, our results support the conclusion that SsPV1/WF-1 causes hypovirulence in Sclerotinia spp. and B. cinerea. Cryo-EM (cryo-electron microscopy) reconstruction of the SsPV1 particle shows that it has a distinct structure with similarity to the closely related partitiviruses Fusarium poae virus 1 and Penicillium stoloniferum virus F. These findings provide new insights into partitivirus biological activities and clues about molecular interactions between partitiviruses and their hosts. IMPORTANCEMembers of the Partitiviridae are believed to occur commonly in their phytopathogenic fungal and plant hosts. However, most partitiviruses examined so far appear to be associated with latent infections. Here we report a partitivirus, SsPV1/WF-1, that was isolated from a hypovirulent strain of Sclerotinia sclerotiorum and describe its biological and molecular features. We have demonstrated that SsPV1 confers hypovirulence. Furthermore, SsPV1 can infect and cause hypovirulence in Botrytis cinerea. Our study also suggests that SsPV1 has a vigorous ability to proliferate and spread via hyphal contact. SsPV1 can overcome vegetative incompatibility barriers and can be transmitted horizontally among different vegetative compatibility groups of S. sclerotiorum, even interspecifically. Cryo-EM reconstruction of SsPV1 shows that it has a distinct structure with similarity to closely related partitiviruses. Our studies exploit a novel system, SsPV1 and its hosts, which can provide the means to explore the mechanisms by which partitiviruses interact with their hosts.

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“…To detect the morphology of virions in the hyphal cells, ultrathin fungal sections of the strain AH98 and Ep-EPNA367-PT2 were prepared to detect the morphology of SsNSRV-1 virions under TEM. These ultrathin fungal sections were prepared and observed in accordance with the method described by Wu et al (36).…”

Section: Methodsmentioning

confidence: 99%

Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses

Liu

Xiè

Chéng

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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191

149

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Significance Mycoviruses are viruses that infect fungi and replicate in fungi. Previously, no mycoviruses had been discovered with negative-stranded (−)ssRNA genomes. Here, we characterize a (−)ssRNA mycovirus that infects a fungal plant pathogen. Although its genome and organization are significantly different from those of current mononegaviruses, this virus is closely related to viruses in families Nyamiviridae and Bornaviridae that infect animals. This discovery may provide insights into the global ecology and evolution of (−)ssRNA viruses. Furthermore, since many (−)ssRNA viruses are serious human pathogens, this system is likely to provide a less hazardous way to study replication of a (−)ssRNA virus and could be useful in establishing a system to screen antiviral compounds against (−)ssRNA viruses.

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Genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus Botrytis cinerea

Cited by 142 publications

References 48 publications

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

Effect of putative mitoviruses on in vitro growth of Gremmeniella abietina isolates under different laboratory conditions

A Novel Partitivirus That Confers Hypovirulence on Plant Pathogenic Fungi

Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses

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