Genome characterization of monkeypox cases detected in India: Identification of three sub clusters among A.2 lineage

Shete, Anita M.; Yadav, Pragya D; Kumar, Abhinendra; Patil, Savita; Patil, Deepak Y.; Joshi, Yash B.; Majumdar, Triparna; Relhan, Vineet; Sahay, Rima R.; Vasu, Meenakshy; Gawande, Pranita; Verma, Ajay; Kumar, Antresh; Dhakad, Shivram; Balakrishnan, Anukumar; Chenayil, Shubin; Kumar, Suresh; Abraham, Priya

doi:10.1016/j.jinf.2022.09.024

Cited by 17 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 1930 total genome sequences, 11 Indian sequences were further selected consisting of five MPXV sequences from New Delhi, India, with no travel history and six sequences from Kerala, India, with recent international travel history. All the cases were symptomatic [ 23 ] and belonged to the A.2 lineage. We examined the proteins with the highest number of nonsynonymous mutations in all 11 sequences.…”

Section: Resultsmentioning

confidence: 99%

Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability

Yadav

Devasurmutt

Tatu

2022

Viruses

View full text Add to dashboard Cite

Monkeypox is an infectious zoonotic disease caused by an Orthopoxvirus and results in symptoms similar to smallpox. In a recent outbreak, monkeypox virus (MPXV) cases have been reported globally since May 2022, and the numbers are increasing. Monkeypox was first diagnosed in humans in the Democratic Republic of Congo and has now spread to throughout Europe, the USA, and Africa. In this study, we analyzed the whole genome sequences of MPXV sequences from recent outbreaks in various countries and performed phylogenomic analysis. Our analysis of the available human MPXV strains showed the highest mutations per sample in 2022 with the average number of mutations per sample being the highest in South America and the European continents in 2022. We analyzed specific mutations in 11 Indian MPXV strains occurring in the variable end regions of the MPXV genome, where the mutation number was as high as 10 mutations per gene. Among these, envelope glycoproteins, the B2R protein, the Ankyrin repeat protein, DNA polymerase, and the INF alpha receptor-like secreted glycoprotein were seen to have a relatively high number of mutations. We discussed the stabilizing effects of the mutations in some of the highly mutating proteins. Our results showed that the proteins involved in binding to the host receptors were mutating at a faster rate, which empowered the virus for active selection towards increased disease transmissibility and severity.

show abstract

Section: Resultsmentioning

confidence: 99%

Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability

Yadav

Devasurmutt

Tatu

2022

Viruses

View full text Add to dashboard Cite

show abstract

“…Phylogenetic analysis suggested the sequences belonged to clade IIb, sublineage A.2.1 (Figure 2). Both sequences carried defined mutations of sublineage A.2.1, including C25072T, A140492C, and C179537T (7). In addition, we found a novel nonsynonymous substitution from threonine to isoleucine in amino 717 (T717I) of the polymerase protein in the sequence from patient 1.…”

Section: Monkeypox Virus Infection In 2 Female Travelers Returning To...mentioning

confidence: 75%

“…Clade IIb is responsible for the ongoing multicountry outbreak, and B.1 is the predominant virus lineage (9). In contrast to lineage B.1, sublineage A.2.1 of clade IIb has only recently been documented in a cluster of persons from India with a travel history to United Arab Emirates (7). In addition, 3 other A.2.1 sequences deposited to GISAID (https://www.gisaid.org) originated in the United Kingdom, the United States, and Nigeria (Figure 1).…”

Section: Monkeypox Virus Infection In 2 Female Travelers Returning To...mentioning

confidence: 99%

Monkeypox Virus Infection in 2 Female Travelers Returning to Vietnam from Dubai, United Arab Emirates, 2022

Dung¹,

Hung²,

Hoa³

et al. 2023

Emerg. Infect. Dis.

View full text Add to dashboard Cite

“…Alarmingly, the clade IIb, responsible for the mpox outbreak in 2022, revealed different lineages in nonendemic countries during the 2022 mpox outbreak based on genetic features, such as A.1. A.1.1, A.2, A.3, and B.1 9–14 . The B.1 lineage is to blame for the present Mpox outbreak.…”

Section: Type Sample Mpox Strain Used Referencesmentioning

confidence: 99%

Mpox virus Clade IIb detection in the air

Saied

2023

Journal of Medical Virology

View full text Add to dashboard Cite

Mpox is a viral zoonotic disease endemic in Central and West Africa that is caused by the Mpox virus, which belongs to the Orthopoxvirus genus and Poxviridae family. The clinical manifestations of mpox infection are milder than those of smallpox, and the incubation time of mpox varies from 5 to 21 days. Since May 2022, the mpox outbreak (formerly known as monkeypox) has suddenly and unexpectedly spread in non-endemic countries, suggesting that there may have been some undetected transmissions. Based on molecular analysis, there are two major genetic clades that represent the mpox virus: Clade I (formerly the Congo Basin clade OR the Central African clade) and Clade II (formerly the West African clade). It is believed that people who are asymptomatic or paucisymptomatic may spread the mpox virus. Infectious viruses cannot be distinguished by PCR testing; therefore, virus culture should be carried out. Recent evidence regarding the detection of the mpox virus (Clade IIb) in air samples collected from the patient's environment during the 2022 mpox outbreak was reviewed. Further studies are needed to evaluate the extent to which the presence of mpox virus DNA in the air could affect immunocompromised patients in healthcare facilities, and further epidemiological studies are crucial, especially in Africa.

show abstract

Genome characterization of monkeypox cases detected in India: Identification of three sub clusters among A.2 lineage

Cited by 17 publications

References 19 publications

Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability

Phylogenomic and Structural Analysis of the Monkeypox Virus Shows Evolution towards Increased Stability

Monkeypox Virus Infection in 2 Female Travelers Returning to Vietnam from Dubai, United Arab Emirates, 2022

Mpox virus Clade IIb detection in the air

Contact Info

Product

Resources

About