Genome-edited powdery mildew resistance in wheat without growth penalties

Li, Shengnan; Lin, Dexing; Zhang, Yunwei; Deng, Min; Chen, Yongxing; Lv, Bin; Li, Boshu; Yuan, Ling; Wang, Yanpeng; Zhao, Long; Liang, Yueting; Liu, Jin-Xing; Chen, Kunling; Liu, Zhiyong; Xiao, Jun; Qiu, Jin‐Long; Gao, Caixia

doi:10.1038/s41586-022-04395-9

Cited by 268 publications

(163 citation statements)

References 63 publications

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“…As of now, no work has been published on the use of genome editing in a banana for resistance to fungal pathogens. However, editing of susceptibility genes such as Mildew resistance locus O ( MLO ), LATERAL ORGAN BOUNDARIES ( CsLOB1 ), DMR6 , ERF922 , amongst others, have been shown to confer resistance to fungal diseases ( Jia et al, 2016 ; Wang et al, 2016 ; Peng et al, 2017 ; de Toledo Thomazella et al, 2021 ; Li et al, 2022 ). These approaches can be adopted in banana to generate resistance against fusarium wilt and black Sigatoka.…”

Section: Potential Applications For Improvement Of Staple Crops In Af...mentioning

confidence: 99%

“…However, the knockout of the MLO gene can lead to growth penalties and yield losses. Recently, Li et al (2022) demonstrated that Tamlo -R32, a mutant with a targeted deletion in the MLO-B1 locus, showed robust resistance to the powdery mildew disease in wheat without impacting its growth and yields.…”

Section: Potential Applications For Improvement Of Staple Crops In Af...mentioning

confidence: 99%

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Genome Editing for Sustainable Agriculture in Africa

et al. 2022

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Sustainable intensification of agriculture in Africa is essential for accomplishing food and nutritional security and addressing the rising concerns of climate change. There is an urgent need to close the yield gap in staple crops and enhance food production to feed the growing population. In order to meet the increasing demand for food, more efficient approaches to produce food are needed. All the tools available in the toolbox, including modern biotechnology and traditional, need to be applied for crop improvement. The full potential of new breeding tools such as genome editing needs to be exploited in addition to conventional technologies. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)-based genome editing has rapidly become the most prevalent genetic engineering approach for developing improved crop varieties because of its simplicity, efficiency, specificity, and easy to use. Genome editing improves crop variety by modifying its endogenous genome free of any foreign gene. Hence, genome-edited crops with no foreign gene integration are not regulated as genetically modified organisms (GMOs) in several countries. Researchers are using CRISPR/Cas-based genome editing for improving African staple crops for biotic and abiotic stress resistance and improved nutritional quality. Many products, such as disease-resistant banana, maize resistant to lethal necrosis, and sorghum resistant to the parasitic plant Striga and enhanced quality, are under development for African farmers. There is a need for creating an enabling environment in Africa with science-based regulatory guidelines for the release and adoption of the products developed using CRISPR/Cas9-mediated genome editing. Some progress has been made in this regard. Nigeria and Kenya have recently published the national biosafety guidelines for the regulation of gene editing. This article summarizes recent advances in developments of tools, potential applications of genome editing for improving staple crops, and regulatory policies in Africa.

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Section: Potential Applications For Improvement Of Staple Crops In Af...mentioning

confidence: 99%

Section: Potential Applications For Improvement Of Staple Crops In Af...mentioning

confidence: 99%

Genome Editing for Sustainable Agriculture in Africa

et al. 2022

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“…Another target for SDN1 editing in wheat is resistance against powdery mildew, which is controlled by Mildew Locus O (MLO), a dominant suppressor of resistance. All three homeoalleles must be knocked out to confer resistance ( Acevedo-Garcia et al, 2017 ; Li et al, 2022 ). Li et al (2022) in fact succeeded in generating mutations in all three loci in elite lines directly, a feat that can be reproduced in other susceptible commercial varieties.…”

Section: No Dearth Of Traits In Crop Plants That Can Be Improved By G...mentioning

confidence: 99%

Gene Editing to Accelerate Crop Breeding

Dhugga

2022

Front. Plant Sci.

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Recent advances in biotechnology have helped increase tissue transformation efficiency and the frequency and specificity of gene editing to an extent that introducing allelic variants directly in elite varieties has become possible. In comparison to the conventional approach of crossing an elite recipient line with an exotic donor parent to introduce the trait of interest followed by repeated backcrossing, direct introduction of major-effect allelic variants into elite varieties saves time and resources, and eliminates yield drag resulting from the residual donor genes at the end of backcrossing.

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“…Barley mlo mutants exhibit pleiotropic phenotypes that can negatively affect harvest yields [ 16 ]. However, it has been demonstrated recently that wheat Tamlo-R32 mutants with a 304-kilobase pair-targeted deletion in the Mlo-B1 locus retain crop growth and yields while conferring robust powdery mildew resistance [ 17 ]. Furthermore, in barley, a number of race-specific, dominant, and semi-dominant R genes (e.g., Mla and Mlg ) [ 18 ] may also confer resistance to Bgh and are associated with the activation of the hypersensitive response (HR), i.e., localized cell death at attempted infection sites [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Near-Isogenic Barley Lines Show Enhanced Susceptibility to Powdery Mildew Infection Following High-Temperature Stress

Nagy

Schwarczinger

Király

et al. 2022

Plants

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Barley cultivation is adversely affected by high-temperature stress, which may modulate plant defense responses to pathogens such as barley powdery mildew (Blumeria graminis f. sp. hordei, Bgh). Earlier research focused mainly on the influence of short-term heat stress (heat shock) of barley on Bgh infection. In this study, our aim was to investigate the effects of both short- and long-term heat stress (35 °C from 30 s to 5 days) on Bgh infection in the barley cultivar Ingrid and its near-isogenic lines containing different powdery mildew resistance genes (Mla12, Mlg, and mlo5) by analyzing symptom severity and Bgh biomass with RT-qPCR. The expression of selected barley defense genes (BAX inhibitor-1, Pathogenesis- related protein-1b, Respiratory burst oxidase homologue F2, and Heat shock protein 90-1) was also monitored in plants previously exposed to heat stress followed by inoculation with Bgh. We demonstrated that pre-exposure to short- and long-term heat stress negatively affects the resistance of all resistant lines manifested by the appearance of powdery mildew symptoms and increased Bgh biomass. Furthermore, prolonged heat stress (48 and 120 h) enhanced both Bgh symptoms and biomass in susceptible wild-type Ingrid. Heat stress suppressed and delayed early defense gene activation in resistant lines, which is a possible reason why resistant barley became partially susceptible to Bgh.

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Genome-edited powdery mildew resistance in wheat without growth penalties

Cited by 268 publications

References 63 publications

Genome Editing for Sustainable Agriculture in Africa

Genome Editing for Sustainable Agriculture in Africa

Gene Editing to Accelerate Crop Breeding

Near-Isogenic Barley Lines Show Enhanced Susceptibility to Powdery Mildew Infection Following High-Temperature Stress

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