Genome engineering Escherichia coli for L-DOPA overproduction from glucose

Wei, Tao; Cheng, Bi-Yan; Liu, Jianzhong

doi:10.1038/srep30080

Cited by 66 publications

(76 citation statements)

References 51 publications

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“…Furthermore, they optimized the pathway by a modular engineering approach and by deleting genes involved in the regulatory and competing pathways, resulting in the titer of salvianic acid A reaching 7.1 g L −1 from glucose in 70 h by fed-batch fermentation. Wei and coworkers 53 first constructed an L-DOPA-producing strain, E. coli DOPA-1, by a singleplex genome engineering approach. The final engineered strain E. coli BKD13 produced 5.6 g L −1 of salvianic acid A by fed-batch fermentation in 60 h from glucose without any antibiotics and inducers.…”

Section: Production Of L-tyr Derivativesmentioning

confidence: 99%

Expanding the repertoire of aromatic chemicals by microbial production

Song

Chen

et al. 2018

J of Chemical Tech & Biotech

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Microbial production of aromatic chemicals would greatly contribute to solving the problems with fossil resource supply and environmentally sustainable development. Engineering and extending the shikimate/aromatic amino acid biosynthetic pathways are important routes for microbial production of various aromatic chemicals. With advances in metabolic engineering and synthetic biology, we can broaden the product spectrum and obtain several valuable and novel aromatic chemicals from renewable feedstocks. Here, in this review, the latest research progress on microbial production of various aromatic chemicals, and recent metabolic engineering and synthetic biology strategies targeting the central carbon metabolism, the shikimate and aromatic amino acid biosynthetic pathways are summarized and discussed. This work aims to provide some valuable tips for the construction of cost‐effective engineered strains for producing various aromatic chemicals. © 2018 Society of Chemical Industry

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Section: Production Of L-tyr Derivativesmentioning

confidence: 99%

Expanding the repertoire of aromatic chemicals by microbial production

Song

Chen

et al. 2018

J of Chemical Tech & Biotech

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“…Another possible explanation is that HCBT used caffeic acid less effectively than p -coumaric acid (Table 3), while the attachment of CoA into either p -coumaric acid or caffeic by 4CL was similar [36]. HpaBC is known to convert tyrosine into L-DOPA (3,4-dihydroxyphenyl- l -alanine) [37], which might inhibit the 4CL or HCBT used in this study. Therefore, we used another approach to synthesize avn F by examining whether the strain HA-Hpa could synthesize avn F from avn D, and then both strains HA-Hpa and HA-S would convert avn D into avn F and then avn E. HpaBC was used for the synthesis of caffeic acid from p -coumaric acid [31] and SOMT9 was used for the methylation of phenolic compounds having vicinal hydroxy groups [38].…”

Section: Resultsmentioning

confidence: 99%

Synthesis of avenanthramides using engineered Escherichia coli

et al. 2018

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BackgroundHydroxycinnamoyl anthranilates, also known as avenanthramides (avns), are a group of phenolic alkaloids with anti-inflammatory, antioxidant, anti-itch, anti-irritant, and antiatherogenic activities. Some avenanthramides (avn A–H and avn K) are conjugates of hydroxycinnamic acids (HC), including p-coumaric acid, caffeic acid, and ferulic acid, and anthranilate derivatives, including anthranilate, 4-hydroxyanthranilate, and 5-hydroxyanthranilate. Avns are primarily found in oat grain, in which they were originally designated as phytoalexins. Knowledge of the avns biosynthesis pathway has now made it possible to synthesize avns through a genetic engineering strategy, which would help to further elucidate their properties and exploit their beneficial biological activities. The aim of the present study was to synthesize natural avns in Escherichia coli to serve as a valuable resource.ResultsWe synthesized nine avns in E. coli. We first synthesized avn D from glucose in E. coli harboring tyrosine ammonia lyase (TAL), 4-coumarate:coenzyme A ligase (4CL), anthranilate N-hydroxycinnamoyl/benzoyltransferase (HCBT), and anthranilate synthase (trpEG). A trpD deletion mutant was used to increase the amount of anthranilate in E. coli. After optimizing the incubation temperature and cell density, approximately 317.2 mg/L of avn D was synthesized. Avn E and avn F were then synthesized from avn D, using either E. coli harboring HpaBC and SOMT9 or E. coli harboring HapBC alone, respectively. Avn A and avn G were synthesized by feeding 5-hydroxyanthranilate or 4-hydroxyanthranilate to E. coli harboring TAL, 4CL, and HCBT. Avn B, avn C, avn H, and avn K were synthesized from avn A or avn G, using the same approach employed for the synthesis of avn E and avn F from avn D.ConclusionsUsing different HCs, nine avns were synthesized, three of which (avn D, avn E, and avn F) were synthesized from glucose in E. coli. These diverse avns provide a strategy to synthesize both natural and unnatural avns, setting a foundation for exploring the biological activities of diverse avns.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0896-9) contains supplementary material, which is available to authorized users.

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“…Indeed, PAAd isplays good selfhealing properties as it contains ah igh concentration of carboxyl functional groups. [17,41,[49][50][51][52] These measurements clearly showedt hat the Si composite electrode film prepared with the PVDF binderh as ah igher resistance to stress and ah igher elastic modulus, but its low elongation indicates that the composite is too brittle compared to those containing PAAa nd Na-CMC. On the contrary,t he composite electrode film with PAN is very ductile, and am ore robust electrode could be expected in the electrochemical cycling tests.…”

Section: Mechanical Properties Of Common Composite Electrodef Ilmsmentioning

confidence: 94%

Effects of the Formulations of Silicon‐Based Composite Anodes on their Mechanical, Storage, and Electrochemical Properties

Assresahegn

Bélanger

2017

ChemSusChem

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In this work, the effects of the formulation of silicon-based composite anodes on their mechanical, storage, and electrochemical properties were investigated. The electrode formulation was changed through the use of hydrogenated or modified (through the covalent attachment of a binding additive such as polyacrylic acid) silicon and acetylene black or graphene sheets as conducting additives. A composite anode with a covalently grafted binder had the highest elongation without breakages and strong adhesion to the current collector. These mechanical properties depend significantly on the conductive carbon additive used and the use of graphene sheets instead of acetylene black can improve elongation and adhesion significantly. After 180 days of storage under ambient conditions, the electronic conductivity and discharge capacity of the modified silicon electrode showed much smaller decreases in these properties than those of the hydrogenated silicon composite electrode, indicating that the modification can result in passivation and a constant composition of the active material. Moreover, the composite Si anode has a high packing density. Consequently, thin-film electrodes with very high material loadings can be prepared without decreased electrochemical performance.

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Genome engineering Escherichia coli for L-DOPA overproduction from glucose

Cited by 66 publications

References 51 publications

Expanding the repertoire of aromatic chemicals by microbial production

Expanding the repertoire of aromatic chemicals by microbial production

Synthesis of avenanthramides using engineered Escherichia coli

Effects of the Formulations of Silicon‐Based Composite Anodes on their Mechanical, Storage, and Electrochemical Properties

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