Genome engineering for breaking barriers in lignocellulosic bioethanol production

Goud, S. L.; Reddy, M. Sreenath; Ulaganathan, Kandasamy

doi:10.1016/j.rser.2017.01.028

Cited by 34 publications

(6 citation statements)

References 536 publications

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“…NADH dehydrogenase is located on the mitochondrial membrane of Z. mobilis , which catalyzes the transfer of electrons from NADH to coenzyme Q. A frameshift mutation in this gene (generating a stop codon at codon 41) resulted in raised ethanol tolerance and yield [17]. The increase in the bioethanol production was dependent mainly on the increase in the tolerance of S. cerevisiae and other potential ethanogenic microorganisms to the inhibitors.…”

Section: Application Of Genome Engineering and Synthetic Biology For mentioning

confidence: 99%

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Genome engineering and synthetic biology for biofuels: A bibliometric analysis

Dai

Wang

Yang

et al. 2020

Biotech and App Biochem

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Genome engineering and synthetic biology as an emerging interdisciplinary tool have opened a new era for energy research as well as life science. In this study, bibliometric and content analysis were conducted to clarify research characteristics and research trends in this field. Our result revealed that USA, China, UK, Germany, and France were the main contributing countries, and USA was in a leading position in international cooperation. The CRISPR‐Cas9 system has been manipulated to develop microorganisms with improved characteristics and tolerance, and is at the forefront of research and practice. In addition, design and construction of synthetic microbial communities and optimization of ecological models to meet industrial demands will become the next hotspot. Meanwhile, effective process configurations that can promote commercial‐scale biofuel production should also be developed. Genome engineering and synthetic biology are expected to continue playing an important role in promoting the development of sustainable energy production.

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Section: Application Of Genome Engineering and Synthetic Biology For mentioning

confidence: 99%

“…Ulaganathan et al. [17] discussed the possibility of addressing the barriers associated with lignocellulose bioethanol production and summarized a number of potential targets for genomic engineering in microorganisms and plants. Javed et al.…”

Section: Introductionmentioning

confidence: 99%

Genome engineering and synthetic biology for biofuels: A bibliometric analysis

Dai

Wang

Yang

et al. 2020

Biotech and App Biochem

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“…O processo de produção de etanol lignocelulósico, também denominado de etanol de 2ª geração, consiste na hidrólise da biomassa, com geração de açúcares fermentescíveis (pentoses e hexoses). [12][13][14] O etanol de cana-de-açúcar se destaca comercialmente, em relação aos demais oriundos de outras matérias-primas, pois possui balanço energético favorável, e um custo aproximadamente nulo de mitigação de carbono. 12,15,16 O etanol obtido a partir de matéria-prima lignocelulósica aparece como uma alternativa para a expansão da contribuição energética da biomassa, além de proporcionar diversos benefícios, como a redução da emissão dos gases responsáveis pelo efeito estufa; contribuição socioeconômica, pois aumenta a renda do produtor e desenvolve as regiões nas quais a disponibilidade de material é grande.…”

Section: Introductionunclassified

Comparison Between Chemical and Enzymatic Hydrolysis of Lignocellulosic Biomass for Bioethanol Production: a Review

Costa¹,

Cruz²,

Rangel³

et al. 2021

Rev. Virtual Quim.

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The dependence on oil and other fossil resources raises global concerns and questions, as they are not considered sustainable from an economic and environmental point of view. The excess availability of residual agricultural material, the growing need for energy in the transport sector and the environmental benefit, due to the possible reduction of greenhouse gases, have boosted the development of liquid biofuels, including second generation bioethanol. Bioethanol is produced by hydrolysis of lignocellulosic materials, to obtain sugars that are later converted into alcohol by fermentation. This process involves the stages of pre-treatment of biomass, saccharification of cellulose, fermentation of hydrolyzate and distillation of alcohol. This work presents and discusses the different technological routes for cellulose hydrolysis, exposing the contrasts between enzymatic hydrolysis and acid hydrolysis, covering parameters such as reaction conditions, cost, yield and applicability. Showing that enzymatic hydrolysis, in general, is superior to homogeneous acid hydrolysis, however, heterogeneous acid hydrolysis presents itself as the most promising hydrolysis route to reduce the weak points found in the production process of this biofuel.A dependência do petróleo e dos demais recursos fósseis gera preocupações e questionamentos globais, em função dos mesmos não serem considerados sustentáveis no ponto de vista econômico e ambiental. A grande disponibilidade de material residual agrícola, a crescente necessidade de energia no setor dos transportes e o benefício ambiental, em função da possível redução dos gases de efeito estufa, impulsionaram o desenvolvimento de biocombustíveis líquidos, entre eles o bioetanol de segunda geração. O bioetanol é produzido a partir da hidrólise de materiais lignocelulósicos, na qual são obtidos açúcares que, posteriormente, são convertidos em álcool por fermentação. Esse processo envolve as etapas de prétratamento da biomassa, a sacarificação da celulose, fermentação do hidrolisado e a destilação do álcool. Esse trabalho apresenta e discute as diferentes rotas tecnológicas para a hidrólise da celulose, expondo os contrastes entre a hidrólise enzimática e a realizada por via ácida, abrangendo parâmetros como, condições reacionais, custo, rendimento e aplicabilidade. Fica evidenciado que a hidrólise enzimática, de forma geral, é mais vantajosa que a hidrólise ácida homogênea. Porém, a hidrólise ácida heterogênea se apresenta como a mais promissora rota com perspectivas de reduzir os pontos fracos existentes no processo produtivo desse biocombustível.

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“…Efforts are underway to break the barriers utilizing the unprecedented tools made available by the genomic revolution sweeping biology recently. Precision genome engineering is the latest among the tools contributed by the field of genomics [6].…”

Section: Introductionmentioning

confidence: 99%

“…Understanding the genomic variations that facilitate high ethanol production by S. cerevisiae is necessary for engineering strains for lignocellulosic bioethanol production. Many strains used in bioethanol production have been sequenced, and a number of variations have been identified [6]. In our effort to select a suitable strain for lignocellulosic bioethanol production, we have sequenced strains differing in their ability to produce bioethanol from plant biomass and reported the genome sequences of a moderate and high ethanol producing strains NCIM3107 and NCIM3186, respectively [12,13,14].…”

Section: Introductionmentioning

confidence: 99%

RNA-seq analysis of transcriptomes for assessing stress tolerance of S. cerevisiae strain, NCIM3186

Burragoni

Ulaganathan

2019

Preprint

Self Cite

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We have previously sequenced the draft genome of high ethanol producing S. cerevisiae strain, NCIM3186. Towards assessing the stress tolerance by this strain transcriptomes from control and in response to glucose, ethanol and furfural stress were sequenced. Comparative RNA-seq analysis of these transcriptomes identified 573 differentially expressed genes of which thiamine biosynthesis genes under furfural stress, TDH1, heat shock proteins and hexose transporter gene under ethanol stress were observed to be highly differentially expressed. Apart from thiamine biosynthesis genes and TDH1, 2 other proteins of unknown function were highly differentially expressed under glucose stress. Most importantly, TAR1 gene was highly down-regulated under all the stress conditions compared to control. Among 93 fermentome genes, 7 (TPS1, TPS2, SIN3, PTK2, SSQ1, ZAP1, DOA4) out of 9 stuck genes are found to be differentially expressed. Several stress-related genes like PHO4, SOD2, STR3, GRE2, GLR1, MEP1,3, MLH3, SNF1, MSN2, ATG1, GLC7 were differentially expressed.

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Genome engineering for breaking barriers in lignocellulosic bioethanol production

Cited by 34 publications

References 536 publications

Genome engineering and synthetic biology for biofuels: A bibliometric analysis

Genome engineering and synthetic biology for biofuels: A bibliometric analysis

Comparison Between Chemical and Enzymatic Hydrolysis of Lignocellulosic Biomass for Bioethanol Production: a Review

RNA-seq analysis of transcriptomes for assessing stress tolerance of S. cerevisiae strain, NCIM3186

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