Genome of a European Fresh-Vegetable Food Safety Outbreak Strain of Salmonella enterica subsp. enterica Serovar Weltevreden

Brankatschk, K.; Blom, Jochen; Goesmann, Alexander; Smits, Theo H. M.; Duffy, Brion

doi:10.1128/jb.00123-11

Cited by 9 publications

(8 citation statements)

References 8 publications

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“…It has long been a major problem associated with meat products in South-East Asia and was also reported as one of the major serotypes in humans in Thailand [31,32]. S. Weltevreden is an emerging serotype associated with meat, seafood and plant products in Western countries [33,34]. In China, especially in the south, the combination of urban consumer groups with large consumption of seafood and poultry products suggests that government should strengthen monitoring and prevention strategies for S. Weltevreden.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization ofSalmonella entericaisolates throughout an integrated broiler supply chain in China

Ren

et al. 2016

Epidemiol. Infect.

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A total of 1145 samples were collected from chicken breeder farms, hatcheries, broiler farms, a slaughterhouse and retail refrigerated chicken stores in an integrated broiler supply chain in Guangdong Province, China, in 2013. One-hundred and two Salmonella enterica strains were isolated and subjected to serotyping, antimicrobial susceptibility testing, virulence profile determination and molecular subtyping by pulsed field gel electrophoresis (PFGE). The contamination rates in samples from breeder farms, hatcheries, broiler farms, the slaughterhouse and retail stores were 1·46%, 4·31%, 7·00%, 62·86% and 54·67%, respectively. The isolated strains of S. enterica belonged to 10 serotypes; most of them were S. Weltevreden (46·08%, 47/102) and S. Agona (18·63%, 19/102). Isolates were frequently resistant to streptomycin (38·2%), tetracycline (36·3%), sulfisoxazole (35·3%) and gentamicin (34·3%); 31·4% of isolates were multidrug resistant. The isolates were screened for 10 virulence factors. The Salmonella pathogenicity island genes avrA, ssaQ, mgtC, siiD, and sopB and the fimbrial gene bcfC were present in 100% of the strains. PFGE genotyping of the 102 S. enterica isolates yielded 24 PFGE types at an 85% similarity threshold. The PFGE patterns show that the genotypes of S. enterica in the production chain are very diverse, but some strains have 100% similarity in different parts of the production chain, which indicates that some S. enterica persist throughout the broiler supply chain.

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Section: Discussionmentioning

confidence: 99%

Prevalence and molecular characterization ofSalmonella entericaisolates throughout an integrated broiler supply chain in China

Ren

et al. 2016

Epidemiol. Infect.

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“…Reads were mapped against the draft genome sequence of S. Weltevreden 2007-60-3289-1 (Brankatschk et al, 2011) using Bowtie 2 (2.0.0-beta2) (Langmead et al, 2009). Generated SAM-files were transcribed into BAMfiles using SAMtools (Li et al, 2009).…”

Section: Mapping and Statistical Analysismentioning

confidence: 99%

“…The genome sequence of S. Weltevreden strain 2007-60-3289-1 consists of 66 contigs that were deposited in the EMBL database under accession numbers FR775188 through FR775253, and the plasmid pSW82 sequence was deposited under accession number FR775255 (Brankatschk et al, 2011). Additional BLAST searches were done at NCBI.…”

Section: Sequence Analysismentioning

confidence: 99%

Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization

Brankatschk

Kamber

Pothier

et al. 2013

Microbial Biotechnology

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Sprouted seeds represent a great risk for infection by human enteric pathogens because of favourable growth conditions for pathogens during their germination. The aim of this study was to identify mechanisms of interactions of Salmonella enterica subsp. enterica Weltevreden with alfalfa sprouts. RNA-seq analysis of S. Weltevreden grown with sprouts in comparison with M9-glucose medium showed that among a total of 4158 annotated coding sequences, 177 genes (4.3%) and 345 genes (8.3%) were transcribed at higher levels with sprouts and in minimal medium respectively. Genes that were higher transcribed with sprouts are coding for proteins involved in mechanisms known to be important for attachment, motility and biofilm formation. Besides gene expression required for phenotypic adaption, genes involved in sulphate acquisition were higher transcribed, suggesting that the surface on alfalfa sprouts may be poor in sulphate. Genes encoding structural and effector proteins of Salmonella pathogenicity island 2, involved in survival within macrophages during infection of animal tissue, were higher transcribed with sprouts possibly as a response to environmental conditions. This study provides insight on additional mechanisms that may be important for pathogen interactions with sprouts.

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“…Salmonella enterica subsp. enterica serovar Weltevreden is an isolate from a gastroenteritis outbreak in Scandinavia associated with contaminated alfalfa sprouts and fresh salad (34). Listeria monocytogenes Scott A is a widely distributed serovar 4b clinical isolate from the 1983 listeriosis outbreak in Massachusetts (35,36).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Aerated Steam Treatment of Alfalfa and Mung Bean Seeds To Eliminate High Levels of Escherichia coli O157:H7 and O178:H12, Salmonella enterica, and Listeria monocytogenes

Studer¹,

Heller²,

Hummerjohann³

et al. 2013

Appl Environ Microbiol

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Sprouts contaminated with human pathogens are able to cause food-borne diseases due to the favorable growth conditions for bacteria during germination and because of minimal processing steps prior to consumption. We have investigated the potential of hot humid air, i.e., aerated steam, to treat alfalfa and mung bean seeds which have been artificially contaminated with Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Weltevreden, and Listeria monocytogenes Scott A. In addition, a recently collected E. coli O178:H12 isolate, characterized by a reduced heat sensitivity, was exposed to the treatment described. Populations of E. coli O157:H7 and S. enterica on alfalfa and mung bean seeds could be completely eliminated by a 300-s treatment with steam at 70 ؎ 1°C as revealed by enrichment studies. L. monocytogenes and E. coli O178:H12 could not be completely eliminated from artificially inoculated seeds. However, bacterial populations were reduced by more than 5 log CFU/g on alfalfa and by more than 4 log CFU/g on mung bean seeds. The germination rate of mung beans was not affected by the 300-s treatment compared to the germination rate of untreated seeds whereas that of alfalfa seeds was significantly lower by 11.9%. This chemical-free method is an effective alternative to the 20,000-ppm hypochlorite treatment presently recommended by the U.S. Food and Drug Administration (FDA).

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Genome of a European Fresh-Vegetable Food Safety Outbreak Strain of Salmonella enterica subsp. enterica Serovar Weltevreden

Cited by 9 publications

References 8 publications

Prevalence and molecular characterization ofSalmonella entericaisolates throughout an integrated broiler supply chain in China

Prevalence and molecular characterization ofSalmonella entericaisolates throughout an integrated broiler supply chain in China

Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization

Evaluation of Aerated Steam Treatment of Alfalfa and Mung Bean Seeds To Eliminate High Levels of Escherichia coli O157:H7 and O178:H12, Salmonella enterica, and Listeria monocytogenes

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