Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members

Sether, D. M.; Melzer, Michael; Borth, Wayne B.; Hu, John

doi:10.1007/s11262-009-0334-5

“…With BYV, CTV and GLRaV-2 (Besse et al, 2009) CPm coats an extremity of the virions, forming a distinct structure for which the terms 'rattlesnake' or 'heterodimeric' were coined. This structure may not be a general characteristic of the family, as suggested by Agranovsky et al (1995) based on the information available at that time, because the ampelovirus species of subgroup II lack a CPm gene (Melzer et al, 2008;Sether et al, 2009; …”

Section: Structure Of Virus Particlesmentioning

confidence: 99%

Closteroviridae

Martelli

¹

,

Candresse²

2014

Encyclopedia of Life Sciences

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Closteroviridae is a family of plant viruses with filamentous, helically constructed particles showing distinct cross‐banding and varying in length from 650 nm (species with fragmented genome) to over 2000 nm (species with monopartite genome). The genome is a single‐stranded, positive‐sense ribonucleic acid , with size varying from 13 000 to nearly 19 000 nucleotides, thus being among the largest known for plant viruses. The family comprises four genera, Closterovirus , Ampelovirus , Crinivirus and Velarivirus , which differ in genome type (monopartite, bipartite or tripartite) and size, and in epidemiological behaviour. Closteroviruses are transmitted by aphids; ampeloviruses by pseudococcid mealybugs and soft scale insects and criniviruses by whiteflies. No vectors are known for velariviruses. These viruses are mostly phloem‐restricted. Except for velariviruses all are transmitted with a semipersistent modality regardless of the type of vector and are pathogenic to a wide range of herbaceous and woody crops, to which they may cause extensive damage. Control is based on preventive measures such as sanitary selection, sanitation and induction of resistance, primarily through conventional breeding. Key Concepts: Closteroviridae is a growing family of plant viruses with currently four genera. All members of the family have filamentous virions with open particle structures and distinct cross‐banding. Closteroviral genomes are among the largest for plant viruses and are highly diversified also within each genus. The family represents a monophyletic lineage that has evolved in a modular way. A characterising trait of three genera ( Closterovirus, Ampelovirus and Crinivirus ) is the type of vectors that mediate transmission from host to host. Virus particles are primarily restricted to the phloem from where they are acquired by the vectors. Virus dissemination over long distances is mediated by infected propagative material, rather than vectors. Most members of the family have a restricted natural host range. Disease control relies on preventive measures aimed at reducing vector populations and sources of inoculum. Sanitary selection and sanitation are efficient means for producing certified stocks.

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“…The isolate of PMWaV-1 detected in this study is similar to that reported in Cuba (Figure 2) due perhaps to the highest trade exchange of propagative material between the two countries and least similar to isolates from Australia and Hawaii related to differences in systems production, cultivated plant material and prevailing environmental conditions in all three cases (Sether et al, 2010). Like PMWaV-1, PMWaV-3 does not induce MWP by itself or together with the mealybug feeding in Hawaii (Sether et al, 2005) and it is unknown what is its effect on the production of pineapple (Sether et al, 2009). Only one plant from the same location that showed different symptoms resulted positive for PMWaV-3.…”

Section: Discussionmentioning

confidence: 78%

“…Así como el PMWaV-1, PMWaV-3 no induce la MaPHP por sí solo ni junto con la alimentación del de piojo harinoso en Hawai (Sether et al, 2005) y no se sabe cuál es el efecto en la producción de piña (Sether et al, 2009 vector management and water supply is neglected since it has been shown that infection by this virus and limited irrigation produce a negative effect (6.7 and 4.2 %, respectively) and an additive effect (13.4 %) on size and weight of the pineapple fruit . The isolate of PMWaV-1 detected in this study is similar to that reported in Cuba (Figure 2) due perhaps to the highest trade exchange of propagative material between the two countries and least similar to isolates from Australia and Hawaii related to differences in systems production, cultivated plant material and prevailing environmental conditions in all three cases (Sether et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The RNA obtained was employed to generate cDNA and use it to realize PCR (MMLV enzyme and Taq polymerase from Promega ® were used) with specific primers of a segment of the hsp70 gen from PMWaV-1 (5'-ACAGGAAGGACAACACTCAC-3'/5'-CGCACAAACTTCAAGCAATC-3'), PMWaV-2 (5'-CATACGAACTAGACTCATACG-3'/5'-TCATTCCACTCACTTATCGTTG-3') and PMWaV-3 (5'-AGTTCACTGTAGATTTCGGA-3'/5'-ATTCATGGATGTGTATCG-3´), that amplify 589, 609 and 495 bp, respectively (Sether et al, 2005;Sether et al, 2009). PCR products were analyzed by electrophoresis on a 2 % agarose gel containing ethidium bromide.…”

Section: Rna Extraction and Rt-pcrmentioning

confidence: 99%

“…El RNA obtenido fue usado para generar cDNA que se usó para realizar PCR (se usaron la enzima MMLV y la Taq polimerasa de Promega ® ) con indicadores específicos de un segmento del gen hsp70 de PMWaV-1 (5'-ACAGGAAGGACAACACT-CAC-3'/5'-CGCACAAACTTCAAGCAATC-3'), PMWaV-2 (5'-CATACGAACTAGACTCATACG-3'/5'-TCATTCCACTCACTTATCGTTG-3') y PMWaV-3 (5'-AGTTCACTGTAGATTTCGGA-3'/5'-ATTCATGGATGTGTATCG-3´), que amplifican 589, 609 y 495 bp, respectivamente (Sether et al, 2005;Sether et al, 2009). Se analizaron los productos PCR por electroforesis en un gel al 2 % de agarosa que contenía bromuro de etidio.…”

Section: Sequence Analysisunclassified

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Detection of Pineapple mealybug wilt-associated virus 1 and 3 in Mexico

Ochoa-MartÃnez

¹

,

Uriza-Ávila

²

,

Rojas-MartÃnez

³

et al. 2016

RMF

5

0

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Abstract. In El Bajo Papaloapan, the main producing area of pineapple of Mexico, leaves with typical symptoms of viral infection consisting in chlorosis, flaccidity, reduced growth and reddening were collected. By RT-PCR with specific primers for the hsp70 gene and subsequent sequencing were detected Pineapple mealybug wilt virus associated-virus 1 (PMWaV-1) and Pineapple mealybug wilt virus associated-3 (PMWaV-3). From the sequences obtained a tree was done with sequences from different regions of the world available in GenBank in order to know their similarity. The sequence obtained from the Mexican isolate PMWaV-1 was genetically related

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Closteroviridae

Martelli¹,

Candresse²

2010

Encyclopedia of Life Sciences

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Closteroviridae is a family of plant viruses with filamentous, helically constructed particles showing distinct cross‐banding and varying in length from 650 nm (species with fragmented genome) to over 2000 nm (species with monopartite genome). The genome is a single‐stranded, positive‐sense RNA ( ribonucleic acid ), with size varying from 13 000 to nearly 19 000 nucleotides, thus being among the largest known for plant viruses. The family comprises three genera, Closterovirus , Ampelovirus and Crinivirus , which differ in genome type (monopartite, bipartite or tripartite) and size, and in epidemiological behaviour. Closteroviruses are transmitted by aphids; ampeloviruses by pseudococcid mealybugs and soft scale insects and criniviruses by whiteflies. These viruses are mostly phloem‐restricted, are all transmitted with a semipersistent modality regardless of the type of vector and are pathogenic to a wide range of herbaceous and woody crops, to which they may cause extensive damage. Control is based on preventive measures such as sanitary selection, sanitation and induction of resistance, primarily through conventional breeding. Key concepts: Closteroviridae is a growing family of plant viruses with three genera. All members of the family have filamentous virions with open particle structures and distinct cross‐banding. Closteroviral genomes are among the largest for plant viruses and are highly diversified also within each genus. The family represents a monophyletic lineage that has evolved in a modular way. A characterizing trait of the genera is the type of vectors that mediate transmission from host to host. Virus particles are primarily restricted to the phloem from where they are acquired by the vectors. Virus dissemination over long distances is mediated by infected propagative material, rather than vectors. Most members of the family have a restricted natural host range. Many cause serious, if not devastating, epidemics to herbaceous and woody crops. Disease control relies on preventive measures aimed at reducing vector populations and sources of inoculum. Sanitary selection and sanitation are efficient means for producing certified stocks.

show abstract

Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members

Cited by 22 publications

References 31 publications

Closteroviridae

Closteroviridae

Detection of Pineapple mealybug wilt-associated virus 1 and 3 in Mexico

Closteroviridae

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