Genome Organization of an Infectious Clone of<i>Tomato Leaf Curl Virus</i>(Philippines), a New Monopartite<i>Begomovirus</i>*

Kon, Tatsuya; Dolores, L.M.; Murayama, Akiko; Bajet, N. B.; Hase, S.; Takahashi, Hideki; Ikegami, Machiko

doi:10.1046/j.1439-0434.2002.00817.x

Cited by 23 publications

(9 citation statements)

References 27 publications

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“…plants ( BK20 and BK30) showing OLCD symptoms were collected from around Bamako and Baguineda, Mali. Leaf samples were squashed onto nylon membranes and total plant DNA was extracted from dried tissue as described previously (Kon et al, 2002;Zhou et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

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Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa

Kon

Rojas

Abdourhamane

et al. 2009

Journal of General Virology

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Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production in West Africa. Two monopartite begomoviruses (okra virus-1 and okra virus-2), a betasatellite and a DNA1 satellite are associated with OLCD in Mali. Okra virus-1 is an isolate of okra yellow crinkle virus (OYCrV), okra virus-2 is a recombinant isolate of cotton leaf curl Gezira virus (CLCuGV) and the betasatellite is a variant of cotton leaf curl Gezira betasatellite (CLCuGB). Cloned DNA of OYCrV and CLCuGV were infectious and induced leaf curl symptoms in Nicotiana benthamiana plants, but did not induce OLCD in okra. However, when these clones were individually co-inoculated with the cloned CLCuGB DNA, symptom severity and viral DNA levels were increased in N. benthamiana plants and typical OLCD symptoms were induced in okra. The CLCuGB was also replicated by, and increased symptom severity of, three monopartite tomato-infecting begomoviruses, including two from West Africa. The sequence of the DNA1 satellite was highly divergent, indicating that it represents a distinct West African lineage. DNA1 replicated autonomously, and replication required the DNA1-encoded Rep protein. Although DNA1 reduced helper begomovirus DNA levels, symptoms were not attenuated. In the presence of CLCuGB, DNA levels of the helper begomoviruses and DNA1 were substantially increased. Together, these findings establish that OLCD in Mali is caused by a complex of monopartite begomoviruses and a promiscuous betasatellite with an associated parasitic DNA1 satellite. These findings are discussed in terms of the aetiology of OLCD and the evolution of new begomovirus/satellite DNA complexes.

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Section: Methodsmentioning

confidence: 99%

“…Total genomic DNA was extracted from leaf tissue as described by Kon et al (2002). Viral and satellite DNA was detected by PCR with the previously described overlapping primer pairs.…”

Section: Methodsmentioning

confidence: 99%

Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa

Kon

Rojas

Abdourhamane

et al. 2009

Journal of General Virology

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“…Total plant DNA was extracted from leaf tissues of whitefly‐inoculated squash plants as described by Kon et al. (2002).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…Sequencing was carried out as described by Kon et al. (2002), and the complete nucleotide compared with those of 26 other geminiviruses.…”

Section: Sequencing and Sequence Analysismentioning

confidence: 99%

Molecular Characterization of a Strain ofSquash Leaf Curl China Virusfrom the Philippines

Kon¹,

Dolores²,

Bajet³

et al. 2003

Journal of Phytopathology

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The complete nucleotide sequence of infectious cloned DNA components (A and B) of the causal agent of squash leaf curl disease in the Philippines was determined. DNA‐A and DNA‐B comprise 2739 and 2705 nucleotides, respectively; the common region is 174 bases in length. Five ORFs were found in DNA‐A and two in DNA‐B. Partial dimeric clones containing DNA‐A and DNA‐B, constructed in a binary vector and transformed into Agrobacterium tumefaciens, induced systemic infection in agro‐inoculated pumpkin plants (Cucurbita moschata). The total DNA‐A sequence was most closely related to that of Squash leaf curl China virus (SLCCNV) (88% identity), although the existence of B component of SLCCNV has not been reported. The deduced coat protein was like that of SLCCNV (98% amino acid sequence identity) and the Philippines virus has low sequence identity to Squash leaf curl virus (SLCV) and Squash mild leaf curl virus (SMLCV) (63 and 64% total nucleotide sequence identities, respectively). From these results, we propose that the Philippines virus be designated Squash leaf curl China virus‐[Philippines] (SLCCNV‐[PH]).

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“…In the tropical and subtropical regions of Asia, begomoviruses, which infect tomato plants, are highly prevalent and greatly hamper tomato production (Green et al 2001;Kon et al 2002;Sukamto et al 2005). In Japan, tomato yields have been variable because of the severe damage by Tomato yellow leaf curl virus (TYLCV), which was first detected in 1996 (Kato et al 1998).…”

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confidence: 99%

Ageratum yellow vein virus isolated from tomato plants with leaf curl on Ishigaki Island, Okinawa, Japan

et al. 2010

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In 2005, severe leaf curling and yellowing were observed on tomato plants on Ishigaki Island. Because the symptoms were consistent with infection by a begomovirus, we used a polymerase chain reaction (PCR) with specific primers for begomovirus DNA-A and DNA satellite component (DNA-b) and detected products of the expected sizes from symptomatic tomato plant samples. DNA sequence analyses of the PCR products revealed that the symptomatic tomato plants were associated with Ageratum yellow vein virus (AYVV) infection. We confirmed AYVV transmission from the naturally infected weed host, Ageratum conyzoides, to healthy tomato plants by the insect vector Bemisia tabaci B biotype. This report is the first of AYVV occurrence in Japan.

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Genome Organization of an Infectious Clone ofTomato Leaf Curl Virus(Philippines), a New MonopartiteBegomovirus*

Cited by 23 publications

References 27 publications

Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa

Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa

Molecular Characterization of a Strain ofSquash Leaf Curl China Virusfrom the Philippines

Ageratum yellow vein virus isolated from tomato plants with leaf curl on Ishigaki Island, Okinawa, Japan

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