Genome-Scale Characterization of Fungal Phytases and a Comparative Study Between Beta-Propeller Phytases and Histidine Acid Phosphatases

Ferreira, Roberta Corsino; Tavares, Murillo Peterlini; Morgan, Túlio; Clevelares, Yan da Silva; Rodrigues, Marina Quádrio Raposo Branco; Kasuya, Maria Catarina Megumi; Mendes, Tiago Antônio de Oliveira; Guimarães, Valéria Monteze

doi:10.1007/s12010-020-03309-7

Cited by 3 publications

(1 citation statement)

References 57 publications

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“…Fungal species from the Fusarium species complex produce phosphatases with significant phytase activity ( Marlida et al, 2010 ; Gontia-Mishra et al, 2014 ; Ferreira et al, 2020 ). Some phytase-producing Fusarium species are important pathogens of crop plants.…”

Section: Introductionmentioning

confidence: 99%

Barley Nepenthesin-Like Aspartic Protease HvNEP-1 Degrades Fusarium Phytase, Impairs Toxin Production, and Suppresses the Fungal Growth

et al. 2021

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Nepenthesins are categorized under the subfamily of the nepenthesin-like plant aspartic proteases (PAPs) that form a distinct group of atypical PAPs. This study describes the effect of nepenthesin 1 (HvNEP-1) protease from barley (Hordeum vulgare L.) on fungal histidine acid phosphatase (HAP) phytase activity. Signal peptide lacking HvNEP-1 was expressed in Pichia pastoris and biochemically characterized. Recombinant HvNEP-1 (rHvNEP-1) strongly inhibited the activity of Aspergillus and Fusarium phytases, which are enzymes that release inorganic phosphorous from phytic acid. Moreover, rHvNEP-1 suppressed in vitro fungal growth and strongly reduced the production of mycotoxin, 15-acetyldeoxynivalenol (15-ADON), from Fusarium graminearum. The quantitative PCR analysis of trichothecene biosynthesis genes (TRI) confirmed that rHvNEP-1 strongly repressed the expression of TRI4, TRI5, TRI6, and TRI12 in F. graminearum. The co-incubation of rHvNEP-1 with recombinant F. graminearum (rFgPHY1) and Fusarium culmorum (FcPHY1) phytases induced substantial degradation of both Fusarium phytases, indicating that HvNEP-1-mediated proteolysis of the fungal phytases contributes to the HvNEP-1-based suppression of Fusarium.

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Section: Introductionmentioning

confidence: 99%

Barley Nepenthesin-Like Aspartic Protease HvNEP-1 Degrades Fusarium Phytase, Impairs Toxin Production, and Suppresses the Fungal Growth

et al. 2021

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Secretomic insight into the biomass hydrolysis potential of the phytopathogenic fungus Chrysoporthe cubensis

Tavares

Morgan

Gomes

et al. 2021

Journal of Proteomics

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Fungal Phytases as Useful Tools in Agricultural Practices

Gocheva,

Stoyancheva,

Miteva-Staleva

et al. 2024

Agronomy

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In order to meet the ever-growing needs of society, modern agriculture must develop sustainable farming and livestock production. Crops need phosphorus, a macronutrient found in soils, but they are only able to utilize small quantities of it. Chemical phosphate fertilizers are ineffective and environmentally harmful. The use of microorganisms (bacteria, yeast, filamentous fungi, and microalgae) that synthesize phytases is a promising and environmentally friendly alternative to chemical fertilizers. Phytases are also needed as feed additives in animal husbandry to overcome phosphorus deficiency for animal growth and development. Phytases are phosphatases that catalyze the release of phosphorus from phytate by stepwise hydrolysis. The broad substrate specificity, optimal pH range, higher thermal stability, and specific efficiency of fungal phytases make them interesting enzymes for agricultural applications. They improve and stimulate the growth and development of plants and animals by releasing inorganic phosphorus and producing siderophores, organic acids, hydrogen cyanide, ammonia, and phytohormones. Phytases are crucial for enhancing phosphorus use in farming and decreasing phosphorus waste’s environmental effects. This paper addresses key challenges in modern farming, such as the inefficient utilization of phosphorus from soil and the environmental harm caused by chemical fertilizers, and provides a comprehensive overview of recent advances in the research of fungal phytases. Available scientific data have been synthesized to highlight the potential of phytase use in agriculture. This review outlines key areas for future research, including the development of phytase variants with improved functionality. The potential integration of fungal phytases into sustainable agricultural practices is underlined, in contrast to previously published work focused primarily on their biochemical properties. The review offers new insight into the possible applications of micromycete phytases as a critical factor for sustainable agriculture in the future.

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Genome-Scale Characterization of Fungal Phytases and a Comparative Study Between Beta-Propeller Phytases and Histidine Acid Phosphatases

Cited by 3 publications

References 57 publications

Barley Nepenthesin-Like Aspartic Protease HvNEP-1 Degrades Fusarium Phytase, Impairs Toxin Production, and Suppresses the Fungal Growth

Barley Nepenthesin-Like Aspartic Protease HvNEP-1 Degrades Fusarium Phytase, Impairs Toxin Production, and Suppresses the Fungal Growth

Secretomic insight into the biomass hydrolysis potential of the phytopathogenic fungus Chrysoporthe cubensis

Fungal Phytases as Useful Tools in Agricultural Practices

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