Genome sequence based, comparative analysis of the fluorescent amplified fragment length polymorphisms (FAFLP) of tubercle bacilli from seals provides molecular evidence for a new species within the Mycobacterium tuberculosis complex

Ahmed, Niyaz; Alam, Mahfooz; Majeed, Ahmed A.; Rahman, Syed Asad; Cataldi, Angel; Cousins, Debby; Hasnain, Seyed E.

doi:10.1016/s1567-1348(02)00100-4

Cited by 23 publications

(23 citation statements)

References 18 publications

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“…Heat-killed cells of isolates from three Australian sea lions, one Australian fur seal and six South American fur seals were digested by using EcoRI/MseI and analysed by FAFLP, using methods described previously (Ahmed et al, 2002(Ahmed et al, , 2003. Analyses were based on the differential amplification of 131 genomic loci.…”

Section: Methodsmentioning

confidence: 99%

“…A recent study of four Australian and six Argentinian seal isolates by fluorescent amplified fragment length polymorphism (FAFLP) has further substantiated the hypothesis that the seal bacillus occupies a unique taxonomic position within the M. tuberculosis complex (Ahmed et al, 2003). This report consolidates the results of tests performed previously and provides additional information, resulting in a comprehensive comparison of isolates available from pinniped-related cases of tuberculosis, and indicates that the seal bacillus should be considered as a novel species of the M. tuberculosis complex.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Cousins

Bastida

Cataldi

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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277

182

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A comparison of Mycobacterium tuberculosis complex isolates from seals (pinnipeds) in Australia, Argentina, Uruguay, Great Britain and New Zealand was undertaken to determine their relationships to each other and their taxonomic position within the complex. Isolates from 30 cases of tuberculosis in six species of pinniped and seven related isolates were compared to representative and standard strains of the M. tuberculosis complex. The seal isolates could be distinguished from other members of the M. tuberculosis complex, including the recently defined 'Mycobacterium canettii ' and 'Mycobacterium caprae', on the basis of host preference and phenotypic and genetic tests. Pinnipeds appear to be the natural host for this 'seal bacillus', although the organism is also pathogenic in guinea pigs, rabbits, humans, Brazilian tapir (Tapirus terrestris) and, possibly, cattle. Infection caused by the seal bacillus is predominantly associated with granulomatous lesions in the peripheral lymph nodes, lungs, pleura, spleen and peritoneum. Cases of disseminated disease have been found. As with other members of the M. tuberculosis complex, aerosols are the most likely route of transmission. The name Mycobacterium pinnipedii sp. nov. is proposed for this novel member of the M. tuberculosis complex (the type strain is 6482 T =ATCC BAA-688 T =NCTC 13288 T ).Abbreviations: BCG, Bacille Calmette-Gué rin; FAFLP, fluorescent amplified fragment length polymorphism; PZA, pyrazinamide; SS, seal spoligotype; TCH, thiophen-2-carboxylic acid hydrazide.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Cousins

Bastida

Cataldi

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

Self Cite

277

182

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“…43,2005 PERFORMANCE OF PCR-BASED TYPING METHODS 5629 (CDFD), Hyderabad, India (1,2). At the CDFD the fragment analysis was performed with GeneScan analysis (version 3.1; Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

“…However, IS6110 restriction fragment length polymorphism (RFLP) has gained recognition as the international standard for epidemiological typing of M. tuberculosis (72,78). More recently, after the genome sequences of various members of the M. tuberculosis complex became available (12,21), variable numbers of tandem repeat (VNTR) typing (56,61,63,65,67), fluorescent amplified fragment length polymorphism (FAFLP) (1,2,23,60), single nucleotide polymorphisms (SNPs) (6,25), and large sequence polymorphisms (10,22,27,31,69) have been developed to differentiate strains.…”

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confidence: 99%

Discriminatory Power and Reproducibility of Novel DNA Typing Methods forMycobacterium tuberculosisComplex Strains

et al. 2005

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In recent years various novel DNA typing methods have been developed which are faster and easier to perform than the current internationally standardized IS6110 restriction fragment length polymorphism typing method. However, there has been no overview of the utility of these novel typing methods, and it is largely unknown how they compare to previously published methods. In this study, the discriminative power and reproducibility of nine recently described PCR-based typing methods for Mycobacterium tuberculosis were investigated using the strain collection of the interlaboratory study of Kremer et al. (J. Clin. Microbiol. 37:2607-2618, 1999). This strain collection contains 90 M. tuberculosis complex and 10 non-M. tuberculosis complex mycobacterial strains, as well as 31 duplicated DNA samples to assess reproducibility. The highest reproducibility was found with variable numbers of tandem repeat typing using mycobacterial interspersed repetitive units (MIRU VNTR) and fast ligation-mediated PCR (FLiP), followed by second-generation spoligotyping, ligation-mediated PCR (LM-PCR), VNTR typing using five repeat loci identified at the Queens University of Belfast (QUB VNTR), and the Amadio speciation PCR. Poor reproducibility was associated with fluorescent amplified fragment length polymorphism typing, which was performed in three different laboratories. The methods were ordered from highest discrimination to lowest by the Hunter-Gaston discriminative index as follows: QUB VNTR typing, MIRU VNTR typing, FLiP, LM-PCR, and spoligotyping. We conclude that both VNTR typing methods and FLiP typing are rapid, highly reliable, and discriminative epidemiological typing methods for M. tuberculosis and that VNTR typing is the epidemiological typing method of choice for the near future.

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“…In principle, the method screens restriction site polymorphism and a clever extension of the primers used during AFLP also facilitates monitoring of DNA polymorphism in the restriction site neighbouring region. This has resulted in the establishment of reproducible and robust microbial typing strategies that do not only provide genetic epidemiological information, but which can also be used to identify new species, even within the M. tuberculosis complex (Ahmed et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Bacillus megaterium Bacteriophages via AFLP Technique

Elmaghraby¹,

Carimi²,

Sharaf³

et al. 2015

Current Research in Bacteriology

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Ten bacteriophages specific for Bacillus megaterium were isolated from a clay loam soil sample collected from the Experimental Farm of Faculty of Agriculture, Minia University, Minia, Egypt. Four out of ten isolates were inactivated after exposure to 80°C for 10 min and three isolates were inactivated at 78°C for 10 min. Whereas, the other three phage isolates were inactivated at 82°C for 10 min. The isolated phages were found to be tolerant to wide range of pH 5-9. The longevity in vitro varied between the phage isolates. The highest longevity in vitro was recorded for four phage isolates (192 h). Electron micrographs of the isolated phages indicated that all phage isolates were of the head and tail types. Two different host specificities were observed for the ten phage isolates (two different populations). Six phage isolates (population 1) were found to be infectious to B. megaterium among the four species tested (i.e., B. megaterium, B. circulans, B. polymexa and B. subtilis). Whereas, the rest of the phage isolates (population 2) were found to be infectious to B. megaterium and B. subtilis. The dendrogram separated the 10 phage isolates into two main clusters (two populations) and then each cluster was separated into two sub clusters. Isolates that belonged to the same host range were grouped together. The percentage of variation was 9% among populations and 91% within populations. The five most remarkable isolates were submitted to the bacillus database and named BMC1, BMC2, BMC3, BMC4 and BMC5.

show abstract

Genome sequence based, comparative analysis of the fluorescent amplified fragment length polymorphisms (FAFLP) of tubercle bacilli from seals provides molecular evidence for a new species within the Mycobacterium tuberculosis complex

Cited by 23 publications

References 18 publications

Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Discriminatory Power and Reproducibility of Novel DNA Typing Methods forMycobacterium tuberculosisComplex Strains

Isolation and Identification of Bacillus megaterium Bacteriophages via AFLP Technique

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