Genome sequence characterization of canine parvoviruses prevalent in the Sichuan province of China

Zhuang, Qingye; Qiu, Yuan; Pan, Zihao; Wang, Su‐chun; Wang, Bo; Wu, Wankun; Yu, Jianmin; Yang, Ying; Sun, Feng-Yan; Wang, Kaicheng

doi:10.1111/tbed.13100

Cited by 35 publications

(30 citation statements)

References 56 publications

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“…In particular, CPV‐2c strains displaying the amino acid glycine (G) instead of the highly conserved alanine (A) at residue 5 of the VP2 have been previously detected in China (Wang et al, ) and Italy (Mira, Purpari, Lorusso, et al, ). More recently, molecular analyses including the NS1 gene sequence showed the presence of molecular signatures of the Asian CPV strains even in this region (Han et al, ; Mira, Purpari, Lorusso, et al, ; Zhuang et al, ). Indeed, it results critical to extend the analysis to other genomic regions to properly infer the spread of the genetic CPV variants (Grecco et al, ).…”

Section: Resultsmentioning

confidence: 97%

“…Whereas CPV‐2a and CPV‐2b are the prevalent variants circulating in Asia (Yi, Tong, Cheng, Song, & Cheng, ), and more recently, CPV‐2c has been described in the same continent (Chiang, Wu, Chiou, Chang, & Lin, ; Geng et al, ; Nakamura et al, ; Wang et al, ; Zhao et al, ; Zhou, Zeng, Zhang, & Li, ; Zhuang et al, ), showing molecular signatures different from those of other continents. Indeed, the Asian CPV‐2c variant shows specific amino acids in NS1 (60V, 544F, 545V, 630P) and VP2 (5A/G, 267Y, 297A, 324I, 370R) gene sequences.…”

Section: Resultsmentioning

confidence: 99%

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Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Mira

Purpari

Colaianni

et al. 2019

Transbound Emerg Dis

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Canine parvovirus type 2 (CPV‐2) emerged as dog pathogen in the late 1970s, causing severe and often fatal epizootics of gastroenteritis in the canine population worldwide. Although to date CPV‐2 is circulating in all continents, most of the current studies have analysed the amino acid changes accounted in the VP2 gene sequence, with limited information on virus introductions from other countries. The aim of this study was to analyse the genetic features of CPV‐2c strains currently spreading in Italy. Swabs and tissue samples were collected from dogs suspected of CPV infection. The nearly complete genome sequence from the CPV‐positive samples was obtained. The co‐circulation of two different but related CPV‐2c strains, with amino acid changes characteristic of CPV strains of Asian origin (NS1: 60V, 544F, 545F, 630P – NS2: 60V, 151N, 152V ‐ VP2: 5A/G, 267Y, 297A, 324I, 370R), were observed. The phylogenetic analyses inferred from the NS1 and VP2 gene sequences confirmed the relationship with Asian CPV‐2c strains. This study reports the spread of novel CPV‐2c mutants in Italy and supports further studies to evaluate the coexistence of genetically divergent CPV strains in the same geographical environment.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Mira

Purpari

Colaianni

et al. 2019

Transbound Emerg Dis

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“…The VP1 aa change was due to a nt change in the second base of the codon (VP1 a347g) and was common to CPV strains of different origin, irrespective of the CPV antigenic variant. Moreover, the VP2 aa change was due to a nt change in the second base of the codon (VP2 c14g), but this change was common only to CPV strains of Asian origin (Mira, Purpari, et al, ; Mira, Purpari, Lorusso, et al, ; Wang et al, ; Zhuang et al, ). The potential biological relevance of these changes has not been described yet and needs to be assessed in further studies.…”

Section: Discussionmentioning

confidence: 99%

“…As most recent Asian CPVs, the Nigerian strains displayed other three aa substitutions in the VP2 sequence (F267Y, Y324I and Q370R). While change at aa residue 324 is predominant in all three CPV variants in Asia (Geng et al, ; Yi, Tong, Cheng, Song, & Cheng, ; Zhao et al, ; Zhou et al, ), the other changes have been less frequently observed, mainly in China since 2013, and change Q370R has been detected only in CPV‐2c strains (Geng et al, ; Guo et al, ; Mira, Purpari, et al, ; Mira, Purpari, Lorusso, et al, ; Wang et al, ; Zhuang et al, ). These aa substitutions are located in the greatest variable VP2 GH loop, comprised between aa 267 and 498, but while residue 267 is not exposed on the capsid surface (Chiang, Wu, Chiou, Chang, & Lin, ) and may not affect the antigenicity of CPV (Xu et al, ), residues 324 and 370 could have immunological implications or biological relevance.…”

Section: Discussionmentioning

confidence: 99%

“…Limited studies are available on the CPV non‐structural genes (Hoelzer et al, ; Pérez et al, ), and, only recently, the analysis of the NS1 gene sequence was included in the CPV phylogenies from several countries (Canuti, Rodrigues, Whitney, & Lang, ; Grecco et al, ; Mira, Canuti, et al, ; Mira, Purpari, et al, ; Zhuang et al, ). In this study, sequence analysis revealed aa changes previously described mainly in NS1/NS2 gene sequences of CPV‐2a/2c strains of Asian origin.…”

Section: Discussionmentioning

confidence: 99%

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Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Ogbu

Mira

Purpari

et al. 2019

Transbound Emerg Dis

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Canine parvovirus type 2 (CPV‐2) emerged suddenly in the late 1970s as pathogen of dogs, causing a severe and often fatal gastroenteric disease. The original CPV‐2 was replaced by three antigenic variants, CPV‐2a, CPV‐2b and CPV‐2c, which to date have gained a worldwide distribution with different relative proportions. All previous studies conducted in Africa were based on partial VP2 gene sequences. The aim of this study was to provide a genome analysis to characterize the CPV strains collected in Nigeria, Africa. Rectal swab samples (n = 320) were collected in 2018 and tested by means of an immunochromatographic assay. Among the 144 positive samples, 59 were selected for further analyses using different molecular assays. The results revealed a high prevalence of CPV‐2c (91.5%) compared to the CPV‐2a variant (8.5%). The VP2 gene sequences showed a divergence from the strains analysed in 2010 in Nigeria and a closer connection with CPV strains of Asian origin. The non‐structural gene analysis evidenced amino acid changes never previously reported. The molecular analysis based on genomic sequences evidenced a geographical pattern of distribution of the analysed strains, suggesting a potential common evolutionary origin with CPV of Asian origin. This study represents the first CPV molecular characterization including all the encoding gene sequences conducted in the African continent and contributes to define the current geographical spread of the CPV variants worldwide.

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Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population

Bui

Hoang

Dung³

et al. 2023

Veterinary Medicine & Sci

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Background: Canine parvovirus type 2 (CPV-2) is known as the primary etiological agent cause of acute gastroenteritis, myocarditis and death of canids worldwide. In Vietnam, although CPV-2 infection and its outbreaks are the most important risk factors of the canine's health concern, lack of available information about the molecular epidemiology of the CPV-2.Objectives: In this study, the complete coding sequences of 10 CPV-2 strains collected from dogs vaccinated with CPV-2 vaccination were analysed to better understand the genomic characteristics of the current circulating CPV-2 in Vietnam. Methods:Ten CPV-specific PCR-positive rectal swab samples were collected from dogs with acute symptoms of haemorrhagic diarrhoea and vomiting in Vietnam in 2019. The complete coding sequences of these CPV strains were analysed to determine their phylogeny and genetic relationship with other available CPV strains globally.Results: Analysis of the VP2 gene sequences demonstrated that the studied strains belonged to the new CPV-2c variants with the unique mutations at amino acids 5 Ala-Gly and 447 Iso-Met . Phylogenetic tree analysis indicated that the studied strains share a common evolutionary origin with the current CPV-2c strains circulating in dogs in Asia countries, including China, Thailand, Taiwan and Mongolia, in recent years. Low sequence identity between the studied strains and commercial vaccine strains was observed. Conclusions:This study provides deep insights into the molecular characteristics, genetic diversity, and evolution of circulating CPV-2 strains in Vietnam. We recommend more studies to estimate the effectiveness of the CPV vaccine and the need to continue developing other effective vaccination essential to better control the widespread of these new CPV-2 variants.

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Genome sequence characterization of canine parvoviruses prevalent in the Sichuan province of China

Cited by 35 publications

References 56 publications

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Molecular characterisation of the current high prevalence of the new CPV‐2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population

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