Genome Sequence of a Highly Virulent pvl-positive Vancomycin intermediate- resistant Staphylococcus aureus Sequence Type 30

Chamon, Raiane Cardoso; Marques, Lucas Miranda; Timenetsky, Jorge; Rachid, Caio Tavora Coelho da Costa; Ferreira, Rosana B. R.; Oliveira, Tamara Lopes Rocha de; Glatthardt, Thaís; Moreira, Luiza Beatriz Melo; Santos, Kátia Regina Netto dos

doi:10.2174/1389202921666200327105756

Cited by 3 publications

(3 citation statements)

References 54 publications

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“…The hVISA phenotype is commonly reported in MRSA strains belonging to ST5, ST8 and ST239. However, the hVISA strain recovered in this research, corresponding to ST30, exhibited similar microbiological characteristics to an hVISA strain isolated from a neonate's blood culture in Brazil in 2009 [10]. The ST30 (CC30) has been circulating in the region and Uruguay since the beginning of the burden of community-acquired MRSA (CA-MRSA) infections and remains present today [12][13][14].…”

Section: Discussionmentioning

confidence: 63%

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Detection of Vancomycin Resistance among Methicillin-Resistant Staphylococcus aureus Strains Recovered from Children with Invasive Diseases in a Reference Pediatric Hospital

Pardo,

Mota,

Parnizari

et al. 2024

Antibiotics

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Vancomycin is the cornerstone intreating methicillin-resistant Staphylococcus aureus (MRSA) infections. However, therapeutic failures can occur when MRSA strains with decreased susceptibility to glycopeptides (DSG) are involved. The aim of this study was to detect and characterize DSG in MRSA recovered from children with invasive diseases at a reference pediatric hospital between 2009 and 2019. Fifty-two MRSA strains were screened using agar plates with vancomycin 3 and 4 mg/L (BHI-3 and BHI-4); the VITEK2 system; and standard and macro E-tests. Suspicious hVISA were studied by population analysis profiling–area under the curve (PAP-AUC), and wall thickness was analyzed by transmission electron microscopy. Neither VRSA nor VISA were detected in this set. As only three strains met the hVISA criteria, the PAP-AUC study included 12 additional MRSA strains that grew one colony on BHI-4 plates or showed minimum inhibitory concentrations of vancomycin and/or teicoplanin ≥ 1.5 mg/L. One strain was confirmed as hVISA by PAP-AUC. The wall thickness was greater than the vancomycin-susceptible control strain; it belonged to ST30 and carried SCCmec IV. As expected, a low frequency of hVISA was found (1.9%). The only hVISA confirmed by PAP-AUC was not detected by the screening methods, highlighting the challenge that its detection represents for microbiology laboratories.

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Section: Discussionmentioning

confidence: 63%

“…However, the hVISA strain #7 was SCCmec IV and did not show a multidrugresistant phenotype. On the other hand, in the neighboring countries Argentina and Brazil, there are descriptions of hVISA strains in both multidrug-resistant and non-multidrugresistant MRSA phenotypes [9][10][11].…”

Section: Discussionmentioning

confidence: 99%

Detection of Vancomycin Resistance among Methicillin-Resistant Staphylococcus aureus Strains Recovered from Children with Invasive Diseases in a Reference Pediatric Hospital

Pardo,

Mota,

Parnizari

et al. 2024

Antibiotics

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“…In a recent study by our group [16], we showed that a H2b PVL-positive USA1100/ST30/CC30 isolate expressed five times more PVL mRNA when compared with other lineages of S. aureus. It is important to point that this isolate was recovered from a neonate bloodstream infection.…”

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confidence: 79%

Panton-Valentine leukocidin (PVL) isoforms among Staphylococcus aureus lineages isolated from hospitals in Rio de Janeiro, Brazil

et al. 2020

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Panton-Valentine leukocidin (PVL) is a Staphylococcus aureus virulence factor codified by lukSF-PV genes. Single-nucleotide polymorphisms (SNPs) at lukSF-PV genes can lead to two PVL sequence variants (R and H) generating different PVL isoforms. This study analyzed lukSF-PV genes SNPs among four different clonal lineages (STs/CC 1, 5, 8, and 30) of nine S. aureus isolated at Brazilian hospitals. The sequenced products showed SNPs at seven sites (positions 121, 470, 527, 663, 856, 1396, and 1729), leading to non-synonymous substitutions in all isolates investigated. Our findings showed new R and H isoforms variants in S. aureus isolated in Brazil and suggest a possible relationship between H2b isoform and the ST30/CC30 lineage. Keywords Staphylococcus aureus. Panton-Valentine leukocidin (PVL) isoforms. Brazilian lineages Panton-Valentine leukocidin (PVL) is a bicomponent and pore-forming toxin that targets phagocytes, leading to membrane damage and cell death in vitro, often associated with recurrent Staphylococcus aureus skin and soft tissue infections and necrotizing pneumonia [1]. PVL can be produced by both methicillin-susceptible (MSSA) and methicillinresistant S. aureus (MRSA) isolates [1, 2]. The USA300/ ST8 MRSA is the commonly PVL-positive lineage found in the USA [3], while in Latin American countries, including Brazil, the USA1100/ST30 MRSA is frequently reported [2, 4]. PVL-codifying genes, lukS-PV and lukF-PV, are carried by a temperate prophage and may be transferred from one isolate to another via phage transduction [5]. Although the PVL genes are considered highly conserved [6], it was demonstrated at least 22 points mutations or SNPs (single-nucleotide polymorphism) in both lukS-PV and lukF-PV [6-11] (Table 1). Based on the nucleotide substitution, the PVL proteins were classified in two major sequence variants of lukSF-PV, named as H or R variants [7]. Despite the recognized role of PVL on S. aureus disease severity [12], the influence of PVL isoforms on protein toxicity and disease outcome remains unknown. Molecular modeling studies suggested that the R isoform may alter pore formation and increase PVL leukotoxicity [7]. On the other hand, some authors suggested that the protein isoform does not influence PVL toxicity, since H and R isoforms could be found at similar frequencies among S. aureus isolates recovered from invasive diseases [6, 13]. However, it is important to point that mutations at virulence-related genes, besides increasing the molecular diversity of bacterial isolates, may lead to gain or loss of protein function. Due to the importance of PVL on S. aureus disease outcome [12], the comprehension of PVL structure and diversity is crucial to understand the microbial pathogenicity and may help the improvement of specific therapeutic drug target design. Moreover, there is no study regarding PVL isoforms of PVLpositive S. aureus isolates from Brazil. Thus, the present study aimed to determine the PVL isoforms of S. aureus previously isolated from different hospitals in Rio de Janeiro [...

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Draft genome sequence of Staphylococcus aureus sequence type 5 SA01 isolated from bloodstream infection and comparative analysis with reference strains

Ferreira,

dos Santos Silva,

Silva

et al. 2023

Funct Integr Genomics

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Genome Sequence of a Highly Virulent pvl-positive Vancomycin intermediate- resistant Staphylococcus aureus Sequence Type 30

Cited by 3 publications

References 54 publications

Detection of Vancomycin Resistance among Methicillin-Resistant Staphylococcus aureus Strains Recovered from Children with Invasive Diseases in a Reference Pediatric Hospital

Detection of Vancomycin Resistance among Methicillin-Resistant Staphylococcus aureus Strains Recovered from Children with Invasive Diseases in a Reference Pediatric Hospital

Panton-Valentine leukocidin (PVL) isoforms among Staphylococcus aureus lineages isolated from hospitals in Rio de Janeiro, Brazil

Draft genome sequence of Staphylococcus aureus sequence type 5 SA01 isolated from bloodstream infection and comparative analysis with reference strains

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